A kind of detection method of alkanes in drinking water

Abstract

The invention belongs to the technical field of detection, and relates to a detection method for alkane in drinking water, and the detection method is a gas chromatography-mass spectrometry combined detection method. The method comprises the following steps: taking drinking water, adding n-hexane, performing extraction treatment, introducing a sample into a gas chromatography-mass spectrometry detector, and quantitatively determining the content of alkane in the drinking water. The detection method is used for detecting the alkane in the drinking water and has good specificity, linear relation, precision, accuracy and repeatability.

Description

technical field

[0001] The invention belongs to the technical field of detection, and relates to a method for detecting alkanes in drinking water, in particular to a method for detecting alkanes in drinking water by gas chromatography-mass spectrometry. Background technique

[0002] Adult body fluid is composed of water, electrolytes, low-molecular organic compounds and proteins, which are widely distributed inside and outside tissue cells, forming the internal environment of the human body. Among them, intracellular fluid accounts for about 40% of body weight, and extracellular fluid accounts for 20% (of which plasma accounts for 5% and interstitial fluid accounts for 15%). Water is an essential substance for the body's material metabolism. Cells must absorb nutrients from the interstitial fluid, and nutrients can be fully absorbed only when they dissolve in water. Intermediate and final products of material metabolism must also be transported and eliminated through the int...

Examples

Embodiment 1

[0029] Embodiment 1 Methodological experiment of the detection method of alkanes in drinking water of the present invention

[0030] Chromatographic conditions:

[0031] 5% phenyl-methyl stationary phase inert chromatographic column (VF-5MS, 30m × 0.25mm (0.25μm), Agilent;) capillary column, the initial column temperature is 40 ° C, keep for 8 minutes, at 100 ° C per minute The temperature was raised to 280°C and maintained for 7 minutes; the temperature of the injection port was 250°C, the carrier gas was helium, the flow rate was linear velocity mode, and the column flow rate was 1ml per minute. Splitless injection, injection 1μl, injection time 1.0 minutes;

[0032] The mass spectrometer adopts EI source, ion source temperature: 200°C, interface temperature: 200°C, SIM mode detection ion (m / z) is 43 (alkane); SCAN mode detection ion (m / z) is 40-500.

[0033] (1) Specificity test

[0034] Blank solvent: n-hexane.

[0035] Positioning solution: Accurately measure 1ml of a...

Embodiment 2

[0080] Sample submitted for inspection: Pulisi Mineral Water, production unit: Jinan Pulisi Mineral Water Co., Ltd.

[0081] Detection method:

[0082] Determined according to gas chromatography (Chinese Pharmacopoeia 2015 Edition Four General Rules 0521) and mass spectrometry (Chinese Pharmacopoeia 2015 Edition Four General Rules 0431).

[0083] Chromatographic conditions: gas phase conditions 5% phenyl-methyl stationary phase inert chromatographic column (VF-5MS, 30m × 0.25mm (0.25μm), Agilent;) capillary column, the initial column temperature is 40 ° C, keep for 8 minutes, The temperature was raised to 280°C at a rate of 100°C per minute and maintained for 7 minutes; the temperature of the injection port was 250°C, the carrier gas was helium, the flow rate was linear velocity mode, and the column flow rate was 1ml per minute. Splitless injection, injection 1μl, injection time 1.0 minutes.

[0084] The mass spectrometry condition adopts EI source, ion source temperature: 2...

Embodiment 3

[0090] Sample submitted for inspection: Nongfu Spring drinking natural water, production unit: Nongfu Spring Co., Ltd.

[0091] Detection method:

[0092] Determined according to gas chromatography (Chinese Pharmacopoeia 2015 Edition Four General Rules 0521) and mass spectrometry (Chinese Pharmacopoeia 2015 Edition Four General Rules 0431).

[0093] Chromatographic conditions: gas phase conditions 5% phenyl-methyl stationary phase inert chromatographic column (VF-5MS, 30m × 0.25mm (0.25μm), Agilent;) capillary column, the initial column temperature is 40 ° C, keep for 8 minutes, The temperature was raised to 280°C at a rate of 100°C per minute and maintained for 7 minutes; the temperature of the injection port was 250°C, the carrier gas was helium, the flow rate was linear velocity mode, and the column flow rate was 1ml per minute. Splitless injection, injection 1μl, injection time 1.0 minutes.

[0094] The mass spectrometry condition adopts EI source, ion source temperature...

technical field

[0001] The invention belongs to the technical field of detection, and relates to a method for detecting alkanes in drinking water, in particular to a method for detecting alkanes in drinking water by gas chromatography-mass spectrometry. Background technique

[0002] Adult body fluid is composed of water, electrolytes, low-molecular organic compounds and proteins, which are widely distributed inside and outside tissue cells, forming the internal environment of the human body. Among them, intracellular fluid accounts for about 40% of body weight, and extracellular fluid accounts for 20% (of which plasma accounts for 5% and interstitial fluid accounts for 15%). Water is an essential substance for the body's material metabolism. Cells must absorb nutrients from the interstitial fluid, and nutrients can be fully absorbed only when they dissolve in water. Intermediate and final products of material metabolism must also be transported and eliminated through the int...

Examples

Embodiment 1

[0029] Embodiment 1 Methodological experiment of the detection method of alkanes in drinking water of the present invention

[0030] Chromatographic conditions:

[0031] 5% phenyl-methyl stationary phase inert chromatographic column (VF-5MS, 30m × 0.25mm (0.25μm), Agilent;) capillary column, the initial column temperature is 40 ° C, keep for 8 minutes, at 100 ° C per minute The temperature was raised to 280°C and maintained for 7 minutes; the temperature of the injection port was 250°C, the carrier gas was helium, the flow rate was linear velocity mode, and the column flow rate was 1ml per minute. Splitless injection, injection 1μl, injection time 1.0 minutes;

[0032] The mass spectrometer adopts EI source, ion source temperature: 200°C, interface temperature: 200°C, SIM mode detection ion (m / z) is 43 (alkane); SCAN mode detection ion (m / z) is 40-500.

[0033] (1) Specificity test

[0034] Blank solvent: n-hexane.

[0035] Positioning solution: Accurately measure 1ml of a...

Embodiment 2

[0080] Sample submitted for inspection: Pulisi Mineral Water, production unit: Jinan Pulisi Mineral Water Co., Ltd.

[0081] Detection method:

[0082] Determined according to gas chromatography (Chinese Pharmacopoeia 2015 Edition Four General Rules 0521) and mass spectrometry (Chinese Pharmacopoeia 2015 Edition Four General Rules 0431).

[0083] Chromatographic conditions: gas phase conditions 5% phenyl-methyl stationary phase inert chromatographic column (VF-5MS, 30m × 0.25mm (0.25μm), Agilent;) capillary column, the initial column temperature is 40 ° C, keep for 8 minutes, The temperature was raised to 280°C at a rate of 100°C per minute and maintained for 7 minutes; the temperature of the injection port was 250°C, the carrier gas was helium, the flow rate was linear velocity mode, and the column flow rate was 1ml per minute. Splitless injection, injection 1μl, injection time 1.0 minutes.

[0084] The mass spectrometry condition adopts EI source, ion source temperature: 2...

Embodiment 3

[0090] Sample submitted for inspection: Nongfu Spring drinking natural water, production unit: Nongfu Spring Co., Ltd.

[0091] Detection method:

[0092] Determined according to gas chromatography (Chinese Pharmacopoeia 2015 Edition Four General Rules 0521) and mass spectrometry (Chinese Pharmacopoeia 2015 Edition Four General Rules 0431).

[0093] Chromatographic conditions: gas phase conditions 5% phenyl-methyl stationary phase inert chromatographic column (VF-5MS, 30m × 0.25mm (0.25μm), Agilent;) capillary column, the initial column temperature is 40 ° C, keep for 8 minutes, The temperature was raised to 280°C at a rate of 100°C per minute and maintained for 7 minutes; the temperature of the injection port was 250°C, the carrier gas was helium, the flow rate was linear velocity mode, and the column flow rate was 1ml per minute. Splitless injection, injection 1μl, injection time 1.0 minutes.

[0094] The mass spectrometry condition adopts EI source, ion source temperature...

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