Bacillus and microbial agent and applications of bacillus and microbial agent

A microbial agent, bacillus technology, applied in the field of microbial fermentation, can solve the problems of low organic waste reduction rate, high dosing frequency, poor adaptability, etc., to achieve the benefits of organic waste, low water content, adaptability good sex effect

Active Publication Date: 2020-05-05
北京中源创能工程技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, my country's small-scale aerobic fermentation technology is imported from Japan, and the bacteria used have been imported from Japan for a long time, and the frequency of dosing is high and the price is expensive, resulting in extremely high operating costs. Moreover, most of China's organic waste is mixed waste, mainly including kitchen waste, For fruit and vegetable waste, garden and agricultural waste, etc., the bacterial agents imported from Japan are not well adapted locally, and the reduction rate of organic waste after fermentation is low and the water content is high

Method used

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  • Bacillus and microbial agent and applications of bacillus and microbial agent
  • Bacillus and microbial agent and applications of bacillus and microbial agent
  • Bacillus and microbial agent and applications of bacillus and microbial agent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0057] According to one embodiment of the present invention, the organic waste may be at least one of the above wastes.

[0058] According to the present invention, the aerobic fermentation can be batch fermentation or continuous fermentation. In order to improve the treatment efficiency more effectively, the method of the aerobic fermentation is continuous fermentation, that is, the materials of the aerobic fermentation are continuously drawn out and fresh organic waste is continuously introduced together with the microbial agent, microbacteria CGMCC NO.18967 or spores Bacillus CGMCC NO.18966 for contact.

[0059] According to the present invention, there is no special restriction on the inoculum amount of microbial bacterial agent, microbacteria CGMCC NO.18967 or bacillus CGMCCNO.18966 in organic waste, a kind of preferred inoculum amount among the present invention, with respect to the organic waste of every gram, The consumption of described microbial bacterial agent, mic...

Embodiment

[0080] The experimental methods used in the following examples are conventional methods unless otherwise specified. The materials, reagents and equipment used in the following examples can be obtained from commercial sources unless otherwise specified.

[0081] The strains used in the following preparation examples:

[0082] Microbacterium (Microbacterium), was preserved on November 19, 2019 in the General Microbiology Center of the China Committee for the Collection of Microbial Cultures (Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese Academy of Sciences, zip code : 100101) (the abbreviation of the depository unit is CGMCC), and the deposit number is CGMCC NO.18967;

[0083] Bacillus was preserved on November 19, 2019 in the General Microbiology Center of China Committee for the Collection of Microbial Cultures (Address: No. 3, Yard 1, Beichen West Road, Chaoyang District, Beijing, Institute of Microbiology, Chinese ...

preparation example 1

[0096]Sterilize the above-mentioned nutrient broth agar medium at 121°C for 20 minutes, and cool to room temperature; draw 200 μL of microbacterium CGMCC NO. Under shaking culture for 24h to activate the strain. Inoculate the activated microbacteria bacteria liquid into 200mL of the above-mentioned new medium, shake and culture it at 30°C and 180rpm for 18h, and obtain the microbacteria seed liquid, the viable bacteria content is 1×10 10 cfu / mL.

[0097] Sterilize the above-mentioned nutrient broth agar medium at 121°C for 20 minutes, cool to room temperature; inoculate 200mL of the above-mentioned medium with 200 μL of Bacillus CGMCC NO. Under shaking culture for 24h to activate the strain. The activated bacillus liquid was inoculated into 200 mL of the above-mentioned new medium, and cultured with shaking at 30° C. and 180 rpm for 22 hours to obtain a bacillus seed liquid. The live bacteria content is 1×10 10 cfu / mL.

[0098] Sterilize the above MRS medium at 121°C for ...

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Abstract

The present invention relates to the field of biological fermentation and discloses a strain of bacillus. The deposit number of the bacillus is CGMCC NO.18966. The present invention also discloses a microbial agent and the microbial agent contains the bacillus CGMCC NO.18966. The provided microbial agent and bacillus CGMCC NO.18966 have strong adaptability and good stability. In an aerobic fermentation device, the used microbial agent or bacillus CGMCC NO.18966 are mixed with organic waste for aerobic fermentation, moisture content of the obtained fermentation product is as low as 18% by weight, and reduction rate is as high as 92% by weight.

Description

technical field [0001] The invention relates to the field of microbial fermentation, in particular to a bacillus, a microbial agent and their application. Background technique [0002] With the development of the economy, the country pays more and more attention to environmental issues, and the rapid increase in the generation of domestic waste has brought huge pressure on environmental protection. For domestic waste, the treatment methods widely used at home and abroad mainly include sanitary landfill, high-temperature composting, and incineration. The proportions of these three main waste treatment methods vary due to factors such as geographical environment, waste composition, and economic development level. . [0003] In my country, organic waste accounts for 40-70% of the total domestic waste. Therefore, in order to reduce or avoid environmental protection problems in the treatment of organic waste, the biological treatment methods based on anaerobic fermentation and a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20B09B3/00B09B5/00C02F11/02C12R1/07
CPCC12N1/20B09B3/00B09B5/00C02F11/02C02F2303/06C12R2001/07C12N1/205
Inventor 王龙黄博魏冬苓张言超阎中魏泉源
Owner 北京中源创能工程技术有限公司
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