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Determination method of B vitamins in beer

A technology of B vitamins and detection methods, which is applied in the field of quality inspection, can solve the problem that B vitamins cannot be fully and simultaneously detected and detected, and achieve the effects of efficient and rapid detection and shortened pretreatment time

Active Publication Date: 2021-01-22
TSINGTAO BREWERY
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

[0004] The present invention aims at the problems that cannot be comprehensively and simultaneously detected and the detection time is long in the detection of the above-mentioned B vitamins, and proposes a fast and simple detection method capable of simultaneously detecting 11 kinds of B vitamins in beer

Method used

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  • Determination method of B vitamins in beer
  • Determination method of B vitamins in beer
  • Determination method of B vitamins in beer

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Experimental program
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Embodiment 1

[0053] 1. Establish a detection method

[0054] 1.1 Preparation of standard solution

[0055] Vitamin B1 standard stock solution: Weigh 0.0250g of vitamin B1 standard product, transfer it with water and dilute it in a 25mL brown measuring bottle, and prepare a concentration of 1000mg / L.

[0056] Vitamin B2 standard stock solution: Weigh 0.0250g of vitamin B2 standard product, dissolve it with ammonia solution, and dilute it into a 25mL brown measuring bottle to prepare a concentration of 1000mg / L.

[0057] Standard stock solutions of vitamin B7 (biotin) and vitamin B9 (folic acid): Weigh 0.0250g of standard biotin and folic acid respectively, add 15mL of ammonia solution to dissolve, adjust the pH value to 7.0 with formic acid, transfer to water and dilute to 25mL brown In the measuring bottle, the preparation concentration is 1000mg / L.

[0058] Vitamin B6 (pyridoxine, pyridoxal, pyridoxamine), vitamin B12, vitamin B3 (niacin, niacinamide), pantothenic acid standard stock so...

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Abstract

The invention provides a method for detecting B vitamins in beer, belongs to the field of quality detection, and can solve the problem that 11 B vitamins in beer cannot be simultaneously detected at present. According to a technical scheme, a liquid chromatography-mass spectrometry method is adopted for detection, standard solutions of 11 B vitamins are prepared, standard curves are made respectively, and quantitative analysis is performed after a beer sample is pretreated; the beer sample pretreatment method comprises the following steps of: putting the beer sample into an ultrafiltration tube, carrying out centrifugal filtration, then adding a hydrochloric acid solution and a BHT solution, and performing filtering for standby use. According to the invention, a detection method for simultaneously detecting 11 vitamins in beer is established, systematic nutrition analysis on the beer is realized through a datamation mode, and the nutrient substances and the nutrient value of the beer are objectively evaluated; by monitoring the change rule of the B vitamins in the brewing process, an optimal brewing means is sought to maintain a highest content of the B vitamins in the beer, and the nutritional value of the beer can be maximized.

Description

technical field [0001] The invention belongs to the field of quality detection, in particular to a method for detecting B vitamins in beer. Background technique [0002] B vitamins are indispensable substances to promote metabolism in the body and convert sugar, fat, protein, etc. into heat. Studies have shown that beer yeast is rich in 11 kinds of B vitamins, and its variety and quantity are unmatched by other natural foods. Beer, as a fermentation product of beer yeast, has continued to grow rapidly in recent years. Therefore, accurate and rapid determination of B vitamins in finished beer The content of group vitamins is of great significance. [0003] At present, the methods for determining B vitamins mainly include microbial methods and high-performance liquid chromatography. Microbiological methods are gradually being replaced by high-performance liquid chromatography due to cumbersome steps and long detection time. Due to the low stability of B vitamins in the exter...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/14
CPCG01N30/02G01N30/06G01N30/14G01N2030/027G01N2030/062G01N2030/146
Inventor 尹花董建军余俊红王剑锋杨朝霞朱蕾张宇昕李梅陈华磊闫鹏田玉红
Owner TSINGTAO BREWERY
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