Pediococcus pentosaceus PP04 with high adhesion capacity and blood fat reducing effect

A technology of Pediococcus pentosaceus and PP04, applied in the directions of chlorella, streptococcus/lactococcus, bacteria used in food preparation, etc., can solve problems such as reduction

Active Publication Date: 2020-06-12
JILIN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through a large number of animal experiments and clinical trials, it has been found that many lactic acid bacteria, such as some Lactobacillus plantarum, Lactobacillus acidophilus, Lactobacillus casei, Lactobacillus rhamnosus, etc., have the effect of

Method used

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  • Pediococcus pentosaceus PP04 with high adhesion capacity and blood fat reducing effect
  • Pediococcus pentosaceus PP04 with high adhesion capacity and blood fat reducing effect
  • Pediococcus pentosaceus PP04 with high adhesion capacity and blood fat reducing effect

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Isolation, purification and identification of Pediococcus pentosaceae PP04

[0030] 1. Isolation and purification of bacterial strains

[0031] The commercially available Northeast sauerkraut was separated from lactic acid bacteria in the laboratory; -1 Gram-positive strains were selected by observing the colony morphology with Gram staining.

[0032] Liquid MRS medium components: peptone 10.0 g, beef extract 10.0 g, yeast extract 5.0 g, diammonium hydrogen citrate [(NH 4 ) 2 HC 6 h 5 o 7 ] 2.0 g, glucose (C 6 h 12 o 6 ·H 2 O) 20.0 g, Tween 80 1.0 mL, sodium acetate (CH 3 COONa·3H 2 O) 5.0 g, dipotassium hydrogen phosphate (K 2 HPO 4 ·3H 2 O) 2.0 g, magnesium sulfate (MgSO4 ·7H 2 O) 0.58g, manganese sulfate (MnSO 4 ·H 2 O) 0.25 g.

[0033] 2. Identification of strains

[0034] The gram-positive strain with better growth vigor isolated by plate streaking method was sent to Shanghai Sangong Company for sequencing, and was identified by 16SrDNA...

Embodiment 2

[0035] Embodiment 2 Domestication of Pediococcus pentosaceae PP04

[0036] Pediococcus pentosaceae PP04 was inoculated with 0.05 mol / L H 2 o 2 , 0.5% (w / v) bile salts and MRS liquid medium at pH 2.0, acclimatized at 37°C for 24 h. Take 200uL of culture solution and spread it on the MRS solid medium, incubate at 37°C for 48 hours, pick a small amount of single colony grown out of the inoculation loop and inoculate it into the MRS liquid medium for rejuvenation, and use 50% (v / v ) glycerol and stored at -80°C for later use.

[0037] Results: After domestication, Pediococcus pentosaceae PP04 could tolerate 0.05 mol / L H 2 o 2 , pH 2.0, 0.5% (w / v) bile salt growth environment is more conducive to its colonization in the host intestine.

Embodiment 3

[0038] Example 3 Pediococcus pentosaceae PP04 tolerance test

[0039] 1. Pediococcus pentosaceae PP04 simulated gastrointestinal fluid tolerance

[0040] 1) Simulation of gastric juice or intestinal juice experiment

[0041] The artificial simulated gastrointestinal juice needs to be freshly prepared. PBS (pH 3.0) was used to prepare pepsin at a concentration of 3 g / L, and filtered through a 0.22 μm filter membrane to prepare the desired simulated gastric juice. Trypsin with a concentration of 1 g / L was prepared in PBS (pH 8.0), and filtered through a 0.22 μm filter membrane to prepare simulated intestinal fluid. Resuspend Pediococcus pentosaceae PP04 with physiological saline, mix the bacterial solution thoroughly in simulated gastric juice (pH 3.0) and adjust its concentration to 1×10 9 CFU / mL, the number of viable bacteria was detected after incubation at 37°C for 3 h. After 3 h, take 1 mL of the culture solution in simulated gastric juice (pH 3.0) and add it to 9 mL of...

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Abstract

The invention discloses a strain of Pediococcus pentosaceus PP04, wherein the preservation number of the Pediococcus pentosaceus PP04 is CCTCC No:M 2019082. A preparation method of fermented soybean milk comprises the following steps: 1) activation of a strain; 2) domestication of the strain: respectively inoculating Pediococcus pentosaceus PP04, streptococcus thermophilus and lactobacillus bulgaricus into a mixed culture solution of skimmed milk and soybean milk, and domesticating at 40-45 DEG C; 3) preparation of fermenting agents: respectively inoculating the domesticated strains into soybean milk according to an inoculation amount of 1-10%, and culturing at 40-45 DEG C to obtain three mother fermenting agents; and 4) preparation of fermented soybean milk: mixing the three mother fermenting agents according to a certain ratio, inoculating the mixture into soybean milk, and fermenting for 3-7 hours. According to the invention, the adhesion rate of Pediococcus pentosaceus PP04 to Caco-2 cells is recorded as 79.06% by a plate coating counting method, and the counting is more accurate than that of a Gram staining method.

Description

technical field [0001] The invention belongs to the technical field of functional food, and specifically relates to a strain of Pediococcus pentosaceae PP04 with high adhesion ability and blood lipid-lowering effect. Background technique [0002] With the improvement of social and economic level, my country's diet structure is gradually tending to the Western diet pattern of high fat and high animal protein, which makes the incidence of hyperlipidemia, also known as hyperlipoproteinemia (hyperlipoproteinemia) increase year by year, and then causes A variety of metabolic disorders, such as obesity, non-alcoholic fatty liver disease, atherosclerosis, coronary heart disease, hypertension, etc. Hyperlipidemia refers to the process of fat metabolism, the level of several lipids in plasma is higher than normal, mainly manifested as serum cholesterol (TC), triglyceride (TG) and low-density lipoprotein cholesterol (LDL-C) higher than normal. At the same time, a large number of stud...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23C11/10A23L2/39A23L33/135C12R1/01A23L11/50A23L11/65
CPCC12N1/20A23C11/106A23L2/39A23L33/135A23V2002/00C12R2001/01C12N1/205A23V2400/123A23V2400/427A23V2400/249A23V2200/3204A23V2200/3262A23V2250/28A23V2250/5062A23V2250/636A23V2300/10A23V2300/20
Inventor 王玉华王宇李侠孙海月田园朴春红于寒松刘俊梅
Owner JILIN AGRICULTURAL UNIV
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