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Thyroid cancer diagnosis prognostic marker MAPK8IP1P2 and application thereof

A technology for thyroid cancer, use, applied in the field of biomedicine

Active Publication Date: 2020-06-12
吉林大学中日联谊医院
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Mitogen-Activated Protein Kinase 8 Interacting Protein 1 Pseudogene 2 (Mitogen-Activated Protein Kinase 8 Interacting Protein 1 Pseudogene 2, MAPK8IP1P2) is a lncRNA, so far there is no clinical significance and biological function of MAPK8IP1P2 in thyroid cancer Related Research Reports

Method used

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  • Thyroid cancer diagnosis prognostic marker MAPK8IP1P2 and application thereof
  • Thyroid cancer diagnosis prognostic marker MAPK8IP1P2 and application thereof
  • Thyroid cancer diagnosis prognostic marker MAPK8IP1P2 and application thereof

Examples

Experimental program
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Effect test

experiment example 1

[0053] Experimental example 1. MAPK8IP1P2 is down-regulated in thyroid cancer tissue, and in the thyroid cancer group with lymph node metastasis The down-regulation in weaving is more obvious

[0054] Such as figure 1 As shown, MAPK8IP1P2 was significantly down-regulated in cancer tissues (8 / 10 cases were downregulated, figure 1 A), further expanding the sample size, it was verified that MAPK8IP1P2 was significantly down-regulated in cancer tissues compared to paracancerous control tissues (ANT in the figure) and benign lesion tissues (Beign in the figure) ( figure 1 B), Interestingly, the downregulation of MAPK8IP1P2 was more significant in the cancer tissues with lymph node metastasis (shown pN1) compared with the cancer tissues without lymph node metastasis (shown pN0) ( figure 1C). The above results show that detecting the level of MAPK8IP1P2 can not only identify whether thyroid cancer is present, but also can distinguish whether thyroid cancer patients have lymph...

experiment example 2

[0055] Experimental example 2. Down-regulation of MAPK8IP1P2 predicts poor prognosis in patients with thyroid cancer

[0056] According to the median expression level of MAPK8IP1P2, the relationship between the expression level of MAPK8IP1P2 and prognosis was further analyzed. The results are as follows: figure 2 As shown, the progression-free survival time of patients in the low expression group was significantly shorter than that in the high expression group, indicating that MAPK8IP1P2 is down-regulated in thyroid cancer tissues and predicts poor prognosis of patients. It can be seen that detecting the level of MAPK8IP1P2 can predict the survival prognosis of patients with thyroid cancer.

experiment example 3

[0057] Experimental example 3. Overexpression of MAPK8IP1P2 can inhibit the tumorigenesis of thyroid cancer cells in vivo

[0058] In B-CPAP and K1 thyroid cancer cells, cell models stably expressing MAPK8IP1P2 and silencing MAPK8IP1P2 were constructed using lentivirus, such as image 3 As shown, cells stably expressing MAPK8IP1P2 were able to up-regulate MAPK8IP1P2, while cells silenced for MAPK8IP1P2 (shown as sh#1) were able to down-regulate MAPK8IP1P2 compared to the respective control vectors. Using the subcutaneous xenograft tumor model in nude mice, we detected the tumorigenesis of K1 cells in vivo, and observed that overexpression of MAPK8IP1P2 in thyroid cancer cells can inhibit the size of tumor formation in vivo, while silencing promotes ( Figure 4 A), analyzing the tumor and growth curve of transplanted tumors, it is also clear that overexpression of MAPK8IP1P2 in thyroid cancer cells can inhibit its tumorigenicity in vivo, while silencing can promote ( Figure...

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Abstract

It is found for the first time that down-regulation is conducted in thyroid cancer tissues, down-regulation is more obvious in thyroid cancer tissues with lymph node metastasis, MAPK8IP1P2 down-regulation indicates poor prognosis of thyroid cancer patients, and overexpression of MAPK8IP1P2 can inhibit in-vivo tumorigenesis of thyroid cancer cells. Based on the new discovery, the MAPK8IP1P2 can beused for diagnosing whether a subject suffers from thyroid cancer or not, lymph node metastasis of thyroid cancer and survival prognosis, thyroid cancer tumorigenesis is inhibited through the medicinefor overexpression of the MAPK8IP1P2, and a new strategy is provided for diagnosis, prognosis and treatment of the thyroid cancer.

Description

technical field [0001] The invention belongs to the field of biomedicine, and more specifically relates to a diagnostic and prognostic marker for thyroid cancer and its application. Background technique [0002] Thyroid cancer is the most common tumor of the endocrine system, and the majority of patients are female, with a male to female ratio of about 1:3. According to pathological types, thyroid cancer is mainly divided into papillary thyroid cancer (PTC), follicular thyroid cancer (FTC), anaplastic thyroid cancer (ATC) and medullary thyroid cancer ( medullary thyroid cancer, MTC). Among them, about 80-90% of thyroid cancers are PTC, which is the most common pathological type. In recent years in my country, with the change of nutritional structure and the trend of aging, the incidence of thyroid cancer has a tendency to increase significantly, and my country has also become a country with a high incidence of thyroid cancer. Among them, the annual percentage change (APC)...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6886A61K45/00A61P35/00
CPCC12Q1/6886A61K45/00A61P35/00C12Q2600/158C12Q2600/178C12Q2600/118
Inventor 刘晓莉孙辉辛精卫张广张纯海
Owner 吉林大学中日联谊医院
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