Application of Carbonyl Iron Sulfur Cluster Nanoparticles in Drug Preparation

A nanoparticle and cluster technology, applied in the field of nanomedicine, can solve the problems of toxicity, malignant tumor treatment effect to be improved, etc., and achieve the effect of less toxic and side effects of organisms

Active Publication Date: 2021-12-14
SHANGHAI NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the therapeutic effect of pure chemodynamic therapy on malignant tumors still needs to be improved.
[0004] CO produced by heme oxygenase at low concentrations in living organisms has cytoprotective activity and, conversely, at very high concentrations is extremely toxic due to its accumulation in tissues

Method used

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  • Application of Carbonyl Iron Sulfur Cluster Nanoparticles in Drug Preparation
  • Application of Carbonyl Iron Sulfur Cluster Nanoparticles in Drug Preparation
  • Application of Carbonyl Iron Sulfur Cluster Nanoparticles in Drug Preparation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] C 8 H 4 Fe 2 O 6 S 2 -Chemokinetic experiments of the response of PSMA-PEG NPs to hydrogen peroxide

[0059] (1) The effect of different pH values: the acetic acid-sodium acetate system was used as the buffer solution, and 3,3',5,5'-tetramethylbenzidine (TMB) was used as the indicator for the detection of hydroxyl radicals ( OH) , add acetic acid-sodium acetate (0.1M, pH=7.4, pH=5.2), C 8 H 4 Fe 2 O 6 S 2 -PSMA-PEG NPs (50 μM, calculated as Fe concentration), TMB solution (0.3 mM), H 2 O 2 solution (0.1mM), the total volume of the above solution is 3mL, mix it evenly and place it in a water bath at 37°C for 50min to test the absorbance of the mixed solution, such as Image 6 shown.

[0060] The tumor microenvironment is a slightly acidic environment (pH=5.2), while in normal tissue it is a neutral environment (pH=7.4). Depend on Image 6 It can be seen that at pH = 5.2 C 8 H 4 Fe 2 O 6 S 2 -PSMA-PEG NPs can react with hydrogen peroxide to genera...

Embodiment 2

[0081] C 8 H 4 Fe 2 O 6 S 2 -PSMA-PEG NPs cell experiment

[0082] (1) Cytotoxicity evaluation: mouse melanoma (B16-F10) cells and human umbilical vein endothelial cells (HUVEC) were selected. The log phase cells were taken, digested with trypsin, and collected by centrifugation after termination, and the cell concentration was 5×10. 4 cells / mL cell suspension, then add 100 μL of cell suspension to each well of the 96-well plate, and incubate in a 37°C incubator for 12h, the cells are fully adherent, and the material is prepared by using high-glucose medium (DMEM). Concentrations for dilution into clusters are: 0, 20, 40, 100, 200, 400 μM C 8 H 4 Fe 2 O 6 S 2 -PSMA-PEG NPs solution. 100 μL of the prepared nanomaterials at different concentrations were added to a 96-well plate (5 parallel wells were set at every concentration), and the 96-well plate was placed in an incubator for further incubation for 12 or 24 hours. After the incubation, suck out the origina...

Embodiment 3

[0090] In vivo therapy experiment

[0091] The treatment was divided into two groups: (1) Control group (Saline) and (2) Treatment group (MNPs), and the concentration of the material injected into the mice was 1.4 mM (calculated as the concentration of the cluster compound), that is, 5.21 mg / kg . Taking the time of intravenous injection of mouse B16-F10 cells as the starting point, denoted as D0, the mice were intravenously injected with nanomaterials on the fourth day to conduct the treatment experiment, followed by the second treatment on the 7th day, and on the 10th day. The third treatment was carried out on the 13th day, the fourth treatment was carried out on the 13th day, and the treatment was terminated on the 16th day. Before each injection of nanomaterials (day 4, day 7, day 10, day 13, day 16), a mouse was dissected, its lungs were removed, the lungs were weighed, photographed, and the mice were examined daily. The mice were monitored for body weight, and the re...

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Abstract

The invention belongs to the technical field of nanomedicine, and discloses the application of carbonyl iron-sulfur cluster nanoparticles in the preparation of medicines, specifically carbonyl iron-sulfur cluster nanoparticles (C 8 h 4 Fe 2 o 6 S 2 ‑PSMA‑PEG NPs) in the preparation and screening of drugs for the treatment / prevention of melanoma lung metastasis, the nanoparticles have carbonyl iron sulfur clusters (C 8 h 4 Fe 2 o 6 S 2 ) as the core and amphiphilic block polymer (PSMA‑PEG) as the coating layer, using its chemical kinetics in the special microenvironment of the tumor and the synergistic effect of CO gas therapy to achieve precise treatment of melanoma lung transfer, and the therapeutic effect is remarkable.

Description

technical field [0001] The invention belongs to the technical field of nanomedicine, in particular to a carbonyl iron-sulfur cluster nanoparticle (C 8 h 4 Fe 2 o 6 S 2 -PSMA-PEG NPs), its preparation method and its application in the preparation and screening of drugs for the treatment / prevention of melanoma lung metastasis. Background technique [0002] At present, there are 100 different types of human cancers known. Malignant melanoma is the most aggressive type of malignant tumors. Generally, local recurrence will not occur, but local and distant metastasis, especially lung metastasis, may occur. However, the current treatment methods chemotherapy and radiotherapy are not ideal for this kind of metastasis, and the side effects are relatively large. Many malignant melanoma patients die of tumor metastasis. Importantly, the treatment and prevention of melanoma metastasis are still major challenges, and with the development of nanotechnology, there is an urgent need to...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K9/51A61K47/34A61K31/555A61K33/00A61P35/00A61P35/04C12Q1/02
CPCA61K9/5146A61K31/555A61K33/00A61P35/00A61P35/04G01N33/5011C12N2503/02G01N2500/10A61K2300/00
Inventor 杨红翟田莉宋云鹏
Owner SHANGHAI NORMAL UNIVERSITY
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