A kind of kit and detection method for the detection of inactivated virus

A virus detection and kit technology, applied in the biological field, can solve the problems of undetectable, easily destroyed, and high infection risk for medical staff and testing personnel, and achieve the effect of preventing virus infection and reducing infection risk.

Active Publication Date: 2022-04-29
THE FOURTH AFFILIATED HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

They all need to collect fresh samples (pharyngeal swabs, sputum, lung perfusate or blood) from patients for testing, but these samples can still pose a great risk of infection to medical staff and testing personnel
Immediately inactivating the collected samples can effectively reduce the risk of infection for medical staff and testing personnel. However, after the samples are inactivated (usually using an autoclave), although the virus loses its original pathogenicity, its Detection markers (DNA, RNA, and proteins with spatial structure) are also easily damaged and cannot be detected

Method used

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  • A kind of kit and detection method for the detection of inactivated virus
  • A kind of kit and detection method for the detection of inactivated virus

Examples

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Effect test

Embodiment 1

[0041] A test kit for inactivated virus detection, comprising the following components: (1) buffer: ammonium bicarbonate solution with a concentration of 50mmol / L, (2) reducing agent: dithiothreitol solution with a concentration of 20mmol / L , (3) alkylating reagent: iodoacetamide solution of concentration 60mmol / L, (4) trypsin: alkaline trypsin, its concentration is 100mg / L, (5) stop solution: the formic acid of mass concentration 60% , (6) Thermostable standard polypeptide; the amino acid sequence of said thermostable standard polypeptide is: LPLGINITNFR (SEQID No.1), and the concentration is 20ng / ml.

Embodiment 2

[0043] A test kit for inactivated virus detection, comprising the following components: (1) buffer: ammonium bicarbonate solution with a concentration of 1000mmol / L, (2) reducing agent: dithiothreitol solution with a concentration of 200mmol / L , (3) alkylating reagent: iodoacetamide solution of concentration 600mmol / L, (4) trypsin: alkaline trypsin, its concentration is 1000mg / L, (5) stop solution: the formic acid of mass concentration 98% , (6) Thermostable standard polypeptide; the amino acid sequence of said thermostable standard polypeptide is: LPLGINITNFR (SEQ ID No.1), and the concentration is 1000ng / ml.

Embodiment 3

[0045] A test kit for inactivated virus detection, comprising the following components: (1) buffer: ammonium bicarbonate solution with a concentration of 500mmol / L, (2) reducing agent: dithiothreitol solution with a concentration of 100mmol / L , (3) alkylation reagent: the iodoacetamide solution of concentration 300mmol / L, (4) trypsin: alkaline trypsin, its concentration is 500mg / L, (5) stop solution: the formic acid of mass concentration 98% , (6) Thermostable standard polypeptide; the amino acid sequence of the thermostable standard polypeptide is: LPLGINITNFR (SEQID No.1), and the concentration is 300ng / ml.

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Abstract

The invention discloses a kit for inactivated virus detection, which comprises the following components: (1) buffer solution, (2) reducing agent, (3) alkylating reagent, (4) trypsin, (5) Stop solution, (6) thermostable standard polypeptide; the amino acid sequence of the thermostable standard polypeptide is: LPLGINITNFR. On the premise of ensuring the detection sensitivity and specificity, the invention can detect the inactivated virus and obtain accurate detection results.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a kit and a detection method for detecting inactivated viruses. Background technique [0002] Viruses are a class of non-cellular microorganisms. The mature and complete virus particle becomes the virion, which is composed of nucleic acid and protein. Viruses are characterized by their small size, which can pass through a sterile filter; simple structure, containing only one type of nucleic acid (DNA or RNA); lack of genes encoding mitochondria and ribosomes, and must be parasitic in living cells and replicated Breed offspring. [0003] Since the first discovery of tobacco mosaic virus in 1892, more than 4,000 species of animal and plant viruses have been discovered, of which more than 500 species are pathogenic to humans. In clinical microbial infections, those caused by viruses account for about 75%. Some infectious guns spread rapidly and widely, with high mortality and serious...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/86G01N33/68
CPCG01N30/02G01N30/8675G01N33/6848Y02A50/30
Inventor 吴建国朱维罡吴伟根吴英萍李游江
Owner THE FOURTH AFFILIATED HOSPITAL OF ZHEJIANG UNIV SCHOOL OF MEDICINE
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