Process for extracting compound from dendrobium nobile and application

Abstract

The invention provides a process for extracting a compound from dendrobium nobile, which comprises the following steps: A) pulverizing dendrobium nobile, leaching with an ethanol water solution, and combining and concentrating a leaching solution to obtain an extract A; B) mixing the extract A with water, extracting with ethyl acetate, and concentrating an ethyl acetate extracting solution to prepare an extract B; C) taking the extract B, enabling the extract B to pass through a decompression column, and carrying out gradient elution to obtain 16 fractions Fr.1-Fr.16; D) enabling the fraction13th Fr.13 to pass through a reversed-phase column, and performing gradient elution to obtain 14 fractions Fr.13-1 to Fr.13-14; and E) passing the seventh fraction Fr.13-14 through a sephadex column,eluting with a mixed solution of methanol and chloroform to obtain 18 fractions Fr.13-7-1 to Fr.13-7-18, combining Fr.13-7-15, Fr.13-7-16, and Fr.13-7-17, and carrying out semi-preparative HPLC purification to obtain the compounds shown in a formula I and a formula II. Researches show that the compounds shown in the formulas I and II can inhibit the activity of alpha-glucosidase and inhibit the generation of NO, and reflect the anti-inflammatory activity. Therefore, the compound can be used for preparing foods and medicines for treating and / or preventing diabetes and inflammation.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a process and application of extracting compounds from Dendrobium nobile. Background technique [0002] In recent years, with the improvement of people's living standards, the prevalence of diabetes has also increased year by year. According to data released by the International Diabetes Federation (IDF) in 2017, there are about 425 million people (20-79 years old) suffering from diabetes worldwide. Diabetes, of which 114 million are in China, ranks first in the world. According to this trend, by 2045, there will be 629 million people worldwide with diabetes. In addition, the incidence of diabetes is as high as 12%, and it is gradually becoming younger. Diabetes mellitus (DM) is a metabolic disorder caused by multiple factors and etiologies, and it is characterized by chronic hyperglycemia. Its pathogenesis is closely related to diet, heredity, environmental factors and immune syst...

AI Technical Summary

Problems solved by technology

At present, steroid hormones and non-steroids (aspirin, diclofenac and ibuprofen, etc.) are often used clinically to treat inflammation, and a series of toxic and side effects will occur after the human body takes them, such as liver and gastrointestinal tract damage and cardiovascular system damage, etc.

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