A kind of technology and application of extracting compound from Dendrobium nobile

A technology of Dendrobium chinensis and compounds, which is applied in the field of extracting compounds from Dendrobium chinensis, can solve problems such as toxic side effects, cardiovascular system damage and the like

Active Publication Date: 2021-07-23
INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, steroid hormones and non-steroids (aspirin, diclofenac and ibuprofen, etc.) are often used clinically to treat inflammation, and a series of toxic and side effects will occur after the human body takes them, such as liver and gastrointestinal tract damage and cardiovascular system damage, etc.

Method used

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  • A kind of technology and application of extracting compound from Dendrobium nobile
  • A kind of technology and application of extracting compound from Dendrobium nobile
  • A kind of technology and application of extracting compound from Dendrobium nobile

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Embodiment 1

[0064] Step 1: After the dried stem (13kg) of Dendrobium nobile is pulverized, it is extracted 3 times with 2 times the volume of ethanol aqueous solution, and the obtained extract is filtered and then merged and concentrated into extract A;

[0065] Step 2: the extract A and water volume ratio 1: 1 are made into suspension, with sherwood oil (extract A: water: petroleum ether=1: 1: 1), ethyl acetate (extract A : water: ethyl acetate = 1: 1: 1), n-butanol (extract A: water: n-butanol = 1: 1: 1) for extraction, each extraction layer was extracted until the extract was colorless and the extraction was stopped. Concentrate the ethyl acetate extract to make extract B;

[0066] Step 3: Take the extract B, pass it through a decompression column, and elute with a gradient mixture of petroleum ether and ethyl acetate (20:1→0:1, V / V), and finally flush the column with acetone, and collect a Each of the obtained fractions was tested by thin-layer chromatography (TLC) spot plate, and si...

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Abstract

The invention provides a process for extracting compounds from Dendrobium nobile, A) crushing Dendrobium nobile, extracting with ethanol aqueous solution, combining and concentrating the extracts to obtain extract A; B) mixing the extract A with water Mix, extract with ethyl acetate, concentrate the ethyl acetate extract to make extract B; C) take extract B, pass through a decompression column, and obtain 16 fractions by gradient elution; D) pass fraction 13 through Reversed-phase column, gradient elution was performed, and 14 fractions were obtained; E) the seventh fraction was passed through a Sephadex column, and eluted with a mixture of methanol and chloroform, and 18 fractions were obtained, and the 15th to 15th fractions were combined. 17 fractions were purified by semi-preparative HPLC to obtain compounds represented by formula I and formula II. The research of the present invention shows that the compounds represented by formulas I and II can inhibit the activity of α-glucosidase and the production of NO, and exhibit anti-inflammatory activity. It shows that the compound can be used to prepare food and medicine for treating and / or preventing diabetes and inflammation.

Description

technical field [0001] The invention belongs to the field of biotechnology, in particular to a process and application of extracting compounds from Dendrobium nobile. Background technique [0002] In recent years, with the improvement of people's living standards, the prevalence of diabetes has also increased year by year. According to data released by the International Diabetes Federation (IDF) in 2017, there are about 425 million people (20-79 years old) suffering from diabetes worldwide. Diabetes, of which 114 million are in China, ranks first in the world. According to this trend, by 2045, there will be 629 million people worldwide with diabetes. In addition, the incidence of diabetes is as high as 12%, and it is gradually becoming younger. Diabetes mellitus (DM) is a metabolic disorder caused by multiple factors and etiologies, and it is characterized by chronic hyperglycemia. Its pathogenesis is closely related to diet, heredity, environmental factors and immune syst...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C231/24C07C235/34A61K31/165A61P3/10A61P29/00
CPCA61P3/10A61P29/00C07C231/24C07C235/34
Inventor 曾军陈惠琴戴好富曹雪梅文莉
Owner INST OF TROPICAL BIOSCI & BIOTECH CHINESE ACADEMY OF TROPICAL AGRI SCI
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