A manganese-oxidizing bacteria that can be used for manganese removal and its application

A technology of manganese oxidation and bacteria is applied in the field of manganese-oxidizing bacteria for efficient demanganese removal.

Active Publication Date: 2022-07-12
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the manganese oxidizing bacteria that have been publicly reported are mainly used in neutral pH conditions, and there are relatively limited reports on the screening of manganese oxidizing bacteria in extremely acidic environments.

Method used

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  • A manganese-oxidizing bacteria that can be used for manganese removal and its application
  • A manganese-oxidizing bacteria that can be used for manganese removal and its application
  • A manganese-oxidizing bacteria that can be used for manganese removal and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1. Screening, isolation and purification of Bacillus sp. MM1

[0045] The screening steps of Bacillus sp. MM1 in this example are as follows:

[0046] (1) Configuration of medium: LB medium, manganese-containing liquid medium, Postage liquid medium and Postage solid medium were configured according to the above formula.

[0047] The production of double-layer Postage solid plate: first pour one layer of Postage solid plate, and immediately after the inoculation operation is completed, pour another layer of plate to cover, this method can make the bacteria grow in a facultative anaerobic environment.

[0048] (2) Enrichment of bacterial sources: The bacterial solution extracted from the acid mine wastewater in Ma'anshan was inoculated into Postage liquid medium at an inoculation ratio of 5% (volume ratio), and cultured at 35°C and 120rpm in a constant temperature shaker for about 15 days in the dark. Then, select the serum bottle with obviously turbid medium and...

Embodiment 2

[0052] Embodiment 2, the identification of manganese oxidizing bacteria

[0053] Morphological identification, biochemical identification and 16S rRNA sequencing analysis of a strain numbered MM1 obtained above:

[0054] 1. The physiological growth form of the bacteria is: dispersed in liquid medium, no biofilm is formed; colonies on solid medium are white, opaque, smooth surface, and regular edges;

[0055] 2. The bacteria are coryneform bacteria, such as figure 2 Scanning electron microscope results are shown.

[0056] 3. If image 3 As shown, the gram identification results are gram-positive bacteria: known gram-positive bacteria are blue-purple, gram-negative bacteria are red, image 3 It is blue-purple after Gram staining, so it is a Gram-positive bacteria.

[0057] 4. The biochemical identification results are: facultative anaerobic, short rod-shaped (0.6-1.2 μm), motility, unable to hydrolyze starch, oxidative glucose oxidative fermentation, contact enzyme positive...

Embodiment 3

[0061] Example 3. Bacillus sp. MM1 is used to treat manganese-containing wastewater with different pH

[0062] The cryopreserved strains were thawed, inoculated into filter-sterilized LB medium, and cultured at 35°C for 1 d at 120 rpm to restore their activity.

[0063] Quantitative manganese sulfate was previously added to the LB medium to an initial manganese ion concentration of 10 mM, and the initial pH was adjusted to 4, 5.5, 7, and 8, respectively. Adjust the amount of inoculum so that the OD600 value of the initial bacterial solution in the medium is 0.1, and set a blank group. Cultivated under the conditions of 35 ° C and 120 rpm, and sampled at 1d, 2d and 3d of cultivation, centrifuged the bacterial liquid, took the supernatant, filtered, and tested the manganese content in it (flame atomic absorption spectrometry), Compared with the blank group, it was confirmed that the bacterial concentration changes and the removal efficiency of manganese elements of the strains ...

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Abstract

The invention discloses a manganese-oxidizing bacterium that can be used for efficient demanganese removal and its application. The bacterium is Bacillus, named Brevibacillus sp.MM1 by classification, and has been preserved in the Guangdong Provincial Microbial Culture Collection Center, and the preservation number is GDMCC NO: 60818, deposited on October 23, 2019. The bacillus (Brevibacillus sp.MM1) of the present invention has high manganese oxidizing ability and can efficiently remove Mn in water or solid substrate under acidic and neutral conditions 2+ At the same time, it can also remove other heavy metals including iron, copper, zinc, chromium, cadmium, arsenic, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and particularly relates to a manganese-oxidizing bacterium that can be used for efficient demanganese removal and its application. Background technique [0002] Due to industrial activities such as mining, electrolysis, and steelmaking, the concentration of manganese ions in water and soil exceeds the standard, which seriously endangers human health. Therefore, demanganese technology has become a research hotspot around the world. [0003] In manganese compounds, the lower the valence state of manganese, the more toxic it is, so Mn 2+ The greatest threat to humanity. Manganese oxidation is a highly active mineralization stage, and manganese oxides are natural strong oxidants, which play an important role in biogeochemical cycles. The oxidation rate of the microbial-mediated manganese oxidation process is much higher than that of abiotic oxidation, and while removing manganese in the en...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/20
CPCC12N1/20C02F3/34B09C1/10C02F2101/206C12R2001/01C12N1/205Y02W10/10
Inventor 王进李平岳正波张林义陈明珠邵锐许庆升
Owner HEFEI UNIV OF TECH
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