A kind of manganese oxidizing bacteria and its screening method and application

A technology of manganese oxidizing bacteria and manganese oxide, which is applied in the field of environmental microorganisms to achieve the effect of low cost and high manganese oxidation ability

Inactive Publication Date: 2020-09-29
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Comparative document 1: the application number is "CN200710175525.9", and the patent name is "manganese oxidizing bacteria Bacillus strain WH4 and its application" discloses a kind of manganese oxidizing bacteria in the biosynthesis of manganese dioxide (MnO 2 ), but when the manganese oxidizing bacteria oxidize manganese, the MnCl of 20mmol / L 2 The MnO formed at the concentration 2 The concentration of manganese oxidizing bacteria is up to 2mmol / L; there is still room for improvement in manganese oxidizing ability; and the fermentation broth of the manganese oxidizing bacteria does not have the ability to remove heavy metals including manganese, copper, zinc, chromium and arsenic at the same time

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  • A kind of manganese oxidizing bacteria and its screening method and application
  • A kind of manganese oxidizing bacteria and its screening method and application
  • A kind of manganese oxidizing bacteria and its screening method and application

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Embodiment 1

[0022] Embodiment 1: Screening of manganese oxidizing bacteria

[0023] The screening method of manganese oxidizing bacteria of the present invention has the following steps:

[0024] (1) Preparation of manganese-containing medium: 5g of yeast extract, 10g of tryptone, 10g of sodium chloride, 1000mL of distilled water, adjust the pH to 7, distribute in 250mL triangular flasks, 100mL in each bottle, and put them in a sterilizing pot 121℃ high temperature steam sterilization for 20min, after cooling, add appropriate amount of filter-sterilized 1mol / L MnCl 2 solution and biological buffer 1mol / L HEPES (pH 7.0), so that the final concentrations of medium Mn(II) and HEPES were 1mmol / L and 20mmol / L, respectively.

[0025] (2) Preliminary screening of manganese oxidizing bacteria: respectively inoculate bacterial liquids of various strains to be screened in the logarithmic phase of growth in the manganese-containing liquid medium of the above-mentioned step S1 according to an inocul...

Embodiment 2

[0030] Embodiment 2: Identification of manganese oxidizing bacteria

[0031] A strain numbered ZM-1 obtained above was identified as Bacillus amyloliquefaciens ZM-1 through morphological identification, biochemical identification and 16S rRNA sequencing analysis;

[0032] 1. The growth physiological form of the bacteria is as follows: a bacterial film is formed in the liquid medium, and the colony on the solid medium is light yellow, opaque, rough on the surface, and irregular in edges;

[0033] 2. If figure 1 Gram identification results shown are Gram-positive bacteria, known Gram-positive bacteria are blue-purple, Gram-negative bacteria are red, figure 1 After Gram staining, it becomes blue-purple, so it is Gram-positive bacteria;

[0034] 3. The results of biochemical identification are aerobic, short rod-shaped (0.7-3.0um), motile, and round spores; positive for nitrate reduction, positive for catalase, positive for hydrolyzed starch, liquefied gelatin, and indole test ...

Embodiment 3

[0039] Implementation 3: Determination of Manganese Oxidation Ability of Manganese Oxidizing Bacteria ZM-1

[0040] (1) Prepare manganese-containing liquid culture medium and distribute them in 250mL Erlenmeyer flasks, 100mL per bottle. Under aseptic operation, add MnCl sterilized by 0.22 μm microporous filtration 2 Solution and HEPES (pH 7.0), make the final concentration of HEPES be 20mmol / L, the final concentration of Mn(II) be 6mmol / L, inoculate the ZM-1 bacterium liquid of growth logarithmic phase by 2% inoculum size.

[0041] (2) Each Erlenmeyer flask was placed in a constant temperature shaker at 30° C. and 180 rpm for shaking culture. Three parallel samples were taken in each group to measure the manganese oxide concentration every 24h until the end of 12d sampling.

[0042] (3) if figure 2 As shown, the manganese oxidation by manganese oxidizing bacteria ZM-1 increased rapidly from 0.01mmol / L at 3d to a maximum of 1.92mmol / L at 10d at 3-10d. The maximum manganese...

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Abstract

The invention provides a manganese oxidizing bacterium, which is named Bacillus amyloliquefaciens ZM‑1 in taxonomy, and its preservation number is: CCTCC NO: M2017851. The invention also provides a preparation method of the manganese oxidizing bacteria and its application in removing pollutant heavy metals. The present invention also provides a manganese oxide for removing heavy metals in pollutants, that is, the fermented liquid obtained by fermenting the above-mentioned manganese oxidizing bacteria, or the dry powder microbial agent obtained by spray drying. The manganese oxidizing bacteria screened have high manganese oxidation ability, and the manganese oxides produced by the manganese oxidizing bacteria have the ability to remove heavy metals copper, zinc, chromium and arsenic; Strong adaptability and no secondary pollution.

Description

technical field [0001] The invention relates to the technical field of environmental microorganisms, in particular to a manganese oxidizing bacteria and its screening method and application. Background technique [0002] Manganese is an abundant metal element in the earth's crust, Mn 2+ It is one of the essential trace elements for the body, but long-term drinking of water containing excessive manganese will cause physiological diseases in the human body. The large-scale mining of manganese ore will inevitably produce a large amount of Mn in this link 2+ emissions and pollution, resulting in Mn in water 2+ pollution. In view of the universality and harmfulness of manganese-containing water, it is necessary to study the technology of manganese removal in drinking water in order to ensure the sanitation and safety of drinking water and improve the quality of life. [0003] my country's "Drinking Water Hygienic Standard" (GB5749-2006) and the "Drinking Water Hygienic Standa...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12Q1/04C02F3/34C01G45/02C12R1/07C02F101/20
CPCC01G45/02C02F3/34C02F2101/206C12Q1/04C12N1/205C12R2001/07
Inventor 肖乃东叶欣周文兵朱端卫蔡建波胡金龙
Owner HUAZHONG AGRI UNIV
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