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Acid-resistant and facultative anaerobic manganese oxidizing bacteria and application thereof

A facultative anaerobic and manganese oxidation technology, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve the problems of low removal rate, limited, and increased violent oxidation.

Active Publication Date: 2020-07-07
HEFEI UNIV OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the manganese oxidizing bacteria reported by the department show good manganese oxidation ability when the manganese ion concentration is lower than 2mM, and most of them do not have manganese oxidation ability when the manganese ion concentration is higher than 30mM
Bacillus sp SG-1 in 10mM Mn 2+ It still has the ability to oxidize under the same conditions, but its removal rate is not high
In addition, it is currently believed that most of the biological manganese oxidation is a strictly aerobic process, and the amount of manganese oxidation will increase with the increase of oxygen concentration
[0005] The manganese oxidizing bacteria that have been publicly reported are mainly used in neutral pH conditions, and there are relatively limited reports on the screening of manganese oxidizing bacteria in extremely acidic environments

Method used

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  • Acid-resistant and facultative anaerobic manganese oxidizing bacteria and application thereof
  • Acid-resistant and facultative anaerobic manganese oxidizing bacteria and application thereof
  • Acid-resistant and facultative anaerobic manganese oxidizing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1, Screening, isolation and purification of Brevibacillus brevis MM2

[0045] The screening steps of Brevibacillus brevis MM2 in the present embodiment are as follows:

[0046] (1) Configuration of medium: LB medium, manganese-containing liquid medium, Postage liquid medium and Postage solid medium were prepared according to the above formula.

[0047] The production of double-layer Postage solid plates: first pour a layer of Postage solid plates, and immediately pour another layer of plates to cover after the inoculation operation is completed. This method can make the bacteria grow in a facultative anaerobic environment.

[0048] (2) Enrichment of bacterial sources: Inoculate the bacterial solution extracted from acid mine wastewater in Ma'anshan into Postage liquid medium at an inoculation ratio of 5% (volume ratio), and incubate in the dark at 35°C and 120rpm on a constant temperature shaker for about 15 days Finally, select the serum bottle whose medium is...

Embodiment 2

[0052] Embodiment 2, the identification of manganese oxidizing bacteria

[0053] Morphological identification, biochemical identification and 16S rRNA sequencing analysis were carried out on the strain numbered MM2 obtained above:

[0054] 1. The physiological growth form of the bacteria is: dispersed in the liquid medium without forming a bacterial film; on the solid medium, the colonies are white, opaque, smooth, and have regular edges;

[0055] 2. The bacterium is a bacillus, such as figure 2 Shown are SEM results.

[0056] 3. If image 3 As shown, the Gram identification result is Gram-positive bacteria: known Gram-positive bacteria are blue-purple, and Gram-negative bacteria are red, image 3 Gram-stained blue-purple, so it is Gram-positive bacteria.

[0057] 4. The results of biochemical identification are: facultative anaerobic, short rod-shaped (0.6-1.2μm), motile, unable to hydrolyze starch, oxidative fermentation of oxidized glucose, positive for catalase, negat...

Embodiment 3

[0061] Embodiment 3, short bacillus (Brevibacillus brevis MM2) is used for processing different pH manganese-containing wastewater

[0062] The frozen preserved strains were thawed, inoculated into filter-sterilized LB medium, and cultured at 35°C at 120rpm for 1d to restore their activity.

[0063] Add quantitative manganese sulfate to the LB medium in advance to an initial manganese ion concentration of 10 mM, and adjust the initial pH to 4, 5.5, 7 and 8, respectively. Adjust the inoculum size so that the OD600 value of the initial bacterial solution in the medium is 0.1, and set up a blank group. Cultivate under the conditions of 35°C and 120rpm of rotating speed, and take samples when culturing 1d, 2d and 3d respectively, centrifuge the bacterial liquid, take the supernatant, filter, test the manganese element content therein (flame atomic absorption spectrometry), Compared with the blank group, confirm the bacterial cell concentration change and the removal efficiency of...

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Abstract

The invention discloses acid-resistant and facultative anaerobic manganese oxidizing bacteria and an application thereof. The bacteria are brevibacillus brevis, which is classified and named as Brevibacillus brevis MM2, has been collected in the Guangdong Microbial Culture Collection Center, has the collection number of GDMCC NO:60819 and is collected in October 23, 2019. The Brevibacillus brevisMM2 is acid-resistant, facultative anaerobic and stronger in environment adaptability, can be used for removing heavy metals in acidic mine wastewater or acidic industrial wastewater, can adapt to wastewater in different dissolved oxygen conditions, and is also suitable for a biological in-situ remediation technology in an oxygen-deficient environment.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and in particular relates to an acid-resistant and facultative anaerobic manganese oxidizing bacterium and its application. Background technique [0002] Manganese is abundant in terrestrial and aquatic systems and is one of the indispensable trace elements for living organisms. However, if the manganese concentration is too high, the human body will be chronically poisoned and cause a variety of cranial nerve diseases. Manganese pollution in various countries in the world mainly includes: water and soil pollution caused by manganese ore wasteland, manganese ore tailings, etc., water and soil pollution caused by manganese-containing wastewater and waste residues produced by electrolytic manganese dioxide, metal manganese, and iron and steel smelting industries. Due to the severity and prevalence of manganese pollution, manganese removal technology has become a research hotspot all over t...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10C02F101/20C02F101/22C02F103/06C02F103/10
CPCC12N1/20C02F3/34C02F2101/206C02F2103/06C02F2103/10C02F2101/20C02F2101/203C02F2101/22C02F2101/103C12R2001/01C12N1/205Y02W10/10
Inventor 岳正波张林义王进陈明珠邵锐许庆升潘鑫
Owner HEFEI UNIV OF TECH
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