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Manganese oxidizing bacteria capable of being used for high-efficiency demanganization and application of manganese oxidizing bacteria

A technology of manganese oxidation and bacteria, which is applied in the field of manganese-oxidizing bacteria for efficient demanganese removal, and can solve problems such as limited

Active Publication Date: 2020-07-07
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the manganese oxidizing bacteria that have been publicly reported are mainly used in neutral pH conditions, and there are relatively limited reports on the screening of manganese oxidizing bacteria in extremely acidic environments.

Method used

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  • Manganese oxidizing bacteria capable of being used for high-efficiency demanganization and application of manganese oxidizing bacteria
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  • Manganese oxidizing bacteria capable of being used for high-efficiency demanganization and application of manganese oxidizing bacteria

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1, Screening and Purification of Bacillus (Brevibacillus sp.MM1)

[0045] The screening steps of bacillus (Brevibacillus sp.MM1) in the present embodiment are as follows:

[0046] (1) Configuration of medium: LB medium, manganese-containing liquid medium, Postage liquid medium and Postage solid medium were prepared according to the above formula.

[0047] The production of double-layer Postage solid plates: first pour a layer of Postage solid plates, and immediately pour another layer of plates to cover after the inoculation operation is completed. This method can make the bacteria grow in a facultative anaerobic environment.

[0048] (2) Enrichment of bacterial sources: Inoculate the bacterial solution extracted from acid mine wastewater in Ma'anshan into Postage liquid medium at an inoculation ratio of 5% (volume ratio), and incubate in the dark at 35°C and 120rpm on a constant temperature shaker for about 15 days Finally, select the serum bottle whose medium...

Embodiment 2

[0052] Embodiment 2, the identification of manganese oxidizing bacteria

[0053] Morphological identification, biochemical identification and 16S rRNA sequencing analysis were carried out on the strain numbered MM1 obtained above:

[0054] 1. The physiological growth form of the bacteria is: dispersed in the liquid medium without forming a bacterial film; on the solid medium, the colonies are white, opaque, smooth, and have regular edges;

[0055] 2. The bacterium is a coryneform bacterium, such as figure 2 Shown are SEM results.

[0056] 3. If image 3 As shown, the Gram identification result is Gram-positive bacteria: known Gram-positive bacteria are blue-purple, and Gram-negative bacteria are red, image 3 Gram-stained blue-purple, so it is Gram-positive bacteria.

[0057] 4. The results of biochemical identification are: facultative anaerobic, short rod-shaped (0.6-1.2μm), motile, unable to hydrolyze starch, oxidative fermentation of oxidized glucose, positive for cat...

Embodiment 3

[0061] Embodiment 3, bacillus (Brevibacillus sp.MM1) is used for processing different pH manganese-containing wastewater

[0062] The frozen preserved strains were thawed, inoculated into filter-sterilized LB medium, and cultured at 35°C at 120rpm for 1d to restore their activity.

[0063] Add quantitative manganese sulfate to the LB medium in advance to an initial manganese ion concentration of 10 mM, and adjust the initial pH to 4, 5.5, 7 and 8, respectively. Adjust the inoculum size so that the OD600 value of the initial bacterial solution in the medium is 0.1, and set up a blank group. Cultivate under the conditions of 35°C and 120rpm of rotating speed, and take samples when culturing 1d, 2d and 3d respectively, centrifuge the bacterial liquid, take the supernatant, filter, test the manganese element content therein (flame atomic absorption spectrometry), Compared with the blank group, confirm the bacterial cell concentration change and the removal efficiency of manganese...

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Abstract

The invention discloses manganese oxidizing bacteria capable of being used for high-efficiency demanganization and an application of the manganese oxidizing bacteria. The bacteria are bacillus, the classification name is Brevibacillus sp.MM1, the Brevibacillus sp.MM1 is preserved in Guangdong Microbial Culture Collection Center, the preservation number is GDMCC NO:60818, and the preservation dateis October 23, 2019. The Brevibacillus sp.MM1 provided by the invention has higher manganese oxidizing ability, and can efficiently remove Mn<2+> in a water body or solid substrate under acidic and neutral conditions, can remove multiple heavy metals such as iron, copper, zinc, chromium, cadmium and arsenic, and has broad application prospects.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and in particular relates to a manganese oxidizing bacterium which can be used for high-efficiency demanganization and its application. Background technique [0002] Due to industrial activities such as mining, electrolysis, and steelmaking, the concentration of manganese ions in water and soil exceeds the standard, which seriously endangers human health. Therefore, manganese removal technology has become a research hotspot all over the world. [0003] In manganese compounds, the lower the valence state of manganese, the more toxic it is, so Mn 2+ greatest threat to humans. Manganese oxidation is a highly active mineralization stage, and manganese oxides are natural strong oxidants, which play an important role in biogeochemical cycles. The oxidation rate of manganese oxidation process mediated by microorganisms is much higher than that of abiotic oxidation, and while removing manganese...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34B09C1/10C12R1/01C02F101/20
CPCC12N1/20C02F3/34B09C1/10C02F2101/206C12R2001/01C12N1/205Y02W10/10
Inventor 王进李平岳正波张林义陈明珠邵锐许庆升
Owner HEFEI UNIV OF TECH
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