An acid-resistant and facultative anaerobic manganese-oxidizing bacteria and its application

A facultative anaerobic and manganese oxidation technology, which is applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of lack of manganese oxidation capacity, increased manganese oxidation, and low removal rate, and achieve adaptability Non-secondary pollution, low cost, and broad application prospects

Active Publication Date: 2022-07-12
HEFEI UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, most of the manganese oxidizing bacteria reported by the department show good manganese oxidation ability when the manganese ion concentration is lower than 2mM, and most of them do not have manganese oxidation ability when the manganese ion concentration is higher than 30mM
Bacillus sp SG-1 in 10mM Mn 2+ It still has the ability to oxidize under the same conditions, but its removal rate is not high
In addition, it is currently believed that most of the biological manganese oxidation is a strictly aerobic process, and the amount of manganese oxidation will increase with the increase of oxygen concentration
[0005] The manganese oxidizing bacteria that have been publicly reported are mainly used in neutral pH conditions, and there are relatively limited reports on the screening of manganese oxidizing bacteria in extremely acidic environments

Method used

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  • An acid-resistant and facultative anaerobic manganese-oxidizing bacteria and its application
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  • An acid-resistant and facultative anaerobic manganese-oxidizing bacteria and its application

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Example 1. Screening, isolation and purification of Brevibacterium

[0045] The screening steps of Brevibacterium in the present embodiment are as follows:

[0046] (1) Configuration of medium: LB medium, manganese-containing liquid medium, Postage liquid medium and Postage solid medium were configured according to the above formula.

[0047] The production of double-layer Postage solid plate: first pour one layer of Postage solid plate, and immediately after the inoculation operation is completed, pour another layer of plate to cover, this method can make the bacteria grow in a facultative anaerobic environment.

[0048] (2) Enrichment of bacterial sources: The bacterial solution extracted from the acid mine wastewater of Ma'anshan was inoculated into Postage liquid medium at an inoculation ratio of 5% (volume ratio), and cultured at 35°C and 120rpm in the dark for about 15 days. Then, select the serum bottle with obviously turbid medium and repeat this step, and repe...

Embodiment 2

[0052] Embodiment 2, the identification of manganese oxidizing bacteria

[0053] Morphological identification, biochemical identification and 16S rRNA sequencing analysis of a strain numbered MM2 obtained above:

[0054] 1. The physiological growth form of the bacteria is: dispersed in liquid medium, no biofilm is formed; colonies on solid medium are white, opaque, smooth surface, and regular edges;

[0055] 2. The bacteria are bacilli, such as figure 2 Scanning electron microscope results are shown.

[0056] 3. If image 3 As shown, the gram identification results are gram-positive bacteria: known gram-positive bacteria are blue-purple, gram-negative bacteria are red, image 3 It is blue-purple after Gram staining, so it is a Gram-positive bacteria.

[0057] 4. The results of biochemical identification are: facultative anaerobic, short rod-shaped (0.6-1.2 μm), motility, unable to hydrolyze starch, oxidative glucose oxidative fermentation, positive for contact enzyme, neg...

Embodiment 3

[0061] Embodiment 3, Brevibacillus is used to treat different pH manganese-containing wastewater

[0062] The cryopreserved strains were thawed, inoculated into filter-sterilized LB medium, and cultured at 35°C for 1 d at 120 rpm to restore their activity.

[0063] Quantitative manganese sulfate was previously added to the LB medium to an initial manganese ion concentration of 10 mM, and the initial pH was adjusted to 4, 5.5, 7, and 8, respectively. Adjust the amount of inoculum so that the OD600 value of the initial bacterial solution in the medium is 0.1, and set a blank group. Cultivated at 35°C and rotating speed of 120rpm, and sampled at 1d, 2d and 3d of culture respectively, centrifuged the bacterial liquid, took the supernatant, filtered, and tested the manganese content in it (flame atomic absorption spectrometry), Compared with the blank group, it was confirmed that the bacterial concentration changes and the removal efficiency of manganese elements of the strains un...

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Abstract

The invention discloses an acid-resistant and facultative anaerobic manganese oxidizing bacterium and its application. The bacterium is Brevibacillus brevis MM2, which is classified and named as Brevibacillus brevis MM2. : 60819, deposited on October 23, 2019. The Brevibacillus brevis MM2 of the present invention has acid resistance and is a facultative anaerobic bacteria, so that it has stronger environmental adaptability, can be used for the removal of heavy metals in acid mine wastewater or acid industrial wastewater, and can adapt to different dissolved oxygen It is also suitable for biological in situ remediation technology in anoxic environments.

Description

technical field [0001] The invention belongs to the field of environmental engineering, and particularly relates to an acid-resistant and facultative anaerobic manganese-oxidizing bacterium and its application. Background technique [0002] Manganese is abundant in terrestrial and aquatic systems and is one of the indispensable trace elements for living organisms. However, if the concentration of manganese is too high, the human body will be chronically poisoned and cause a variety of neurological diseases. Manganese pollution in various countries in the world mainly includes: water and soil pollution caused by manganese ore waste, manganese ore tailings, etc., and water and soil pollution caused by manganese-containing wastewater and waste residues generated by electrolytic manganese dioxide and metal manganese, iron and steel smelting and other industries. Due to the seriousness and universality of manganese pollution, manganese removal technology has become a research ho...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01C02F101/10C02F101/20C02F101/22C02F103/06C02F103/10
CPCC12N1/20C02F3/34C02F2101/206C02F2103/06C02F2103/10C02F2101/20C02F2101/203C02F2101/22C02F2101/103C12R2001/01C12N1/205Y02W10/10
Inventor 岳正波张林义王进陈明珠邵锐许庆升潘鑫
Owner HEFEI UNIV OF TECH
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