An acid-resistant and facultative anaerobic manganese-oxidizing bacteria and its application
A facultative anaerobic and manganese oxidation technology, which is applied in the direction of bacteria, chemical instruments and methods, and methods based on microorganisms, can solve the problems of lack of manganese oxidation capacity, increased manganese oxidation, and low removal rate, and achieve adaptability Non-secondary pollution, low cost, and broad application prospects
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Embodiment 1
[0044] Example 1. Screening, isolation and purification of Brevibacterium
[0045] The screening steps of Brevibacterium in the present embodiment are as follows:
[0046] (1) Configuration of medium: LB medium, manganese-containing liquid medium, Postage liquid medium and Postage solid medium were configured according to the above formula.
[0047] The production of double-layer Postage solid plate: first pour one layer of Postage solid plate, and immediately after the inoculation operation is completed, pour another layer of plate to cover, this method can make the bacteria grow in a facultative anaerobic environment.
[0048] (2) Enrichment of bacterial sources: The bacterial solution extracted from the acid mine wastewater of Ma'anshan was inoculated into Postage liquid medium at an inoculation ratio of 5% (volume ratio), and cultured at 35°C and 120rpm in the dark for about 15 days. Then, select the serum bottle with obviously turbid medium and repeat this step, and repe...
Embodiment 2
[0052] Embodiment 2, the identification of manganese oxidizing bacteria
[0053] Morphological identification, biochemical identification and 16S rRNA sequencing analysis of a strain numbered MM2 obtained above:
[0054] 1. The physiological growth form of the bacteria is: dispersed in liquid medium, no biofilm is formed; colonies on solid medium are white, opaque, smooth surface, and regular edges;
[0055] 2. The bacteria are bacilli, such as figure 2 Scanning electron microscope results are shown.
[0056] 3. If image 3 As shown, the gram identification results are gram-positive bacteria: known gram-positive bacteria are blue-purple, gram-negative bacteria are red, image 3 It is blue-purple after Gram staining, so it is a Gram-positive bacteria.
[0057] 4. The results of biochemical identification are: facultative anaerobic, short rod-shaped (0.6-1.2 μm), motility, unable to hydrolyze starch, oxidative glucose oxidative fermentation, positive for contact enzyme, neg...
Embodiment 3
[0061] Embodiment 3, Brevibacillus is used to treat different pH manganese-containing wastewater
[0062] The cryopreserved strains were thawed, inoculated into filter-sterilized LB medium, and cultured at 35°C for 1 d at 120 rpm to restore their activity.
[0063] Quantitative manganese sulfate was previously added to the LB medium to an initial manganese ion concentration of 10 mM, and the initial pH was adjusted to 4, 5.5, 7, and 8, respectively. Adjust the amount of inoculum so that the OD600 value of the initial bacterial solution in the medium is 0.1, and set a blank group. Cultivated at 35°C and rotating speed of 120rpm, and sampled at 1d, 2d and 3d of culture respectively, centrifuged the bacterial liquid, took the supernatant, filtered, and tested the manganese content in it (flame atomic absorption spectrometry), Compared with the blank group, it was confirmed that the bacterial concentration changes and the removal efficiency of manganese elements of the strains un...
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