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Kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics and detection method thereof

A technology of glycopeptide antibiotics and antifungal drugs, which is applied in the field of clinical detection, can solve problems such as large differences in physical and chemical properties, and achieve the effect of extracting many types of compounds, high accuracy, and improving detection efficiency

Pending Publication Date: 2020-07-10
AUTOBIO DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because the physicochemical properties of triazole antifungal drugs and glycopeptide antibiotics are quite different, there are no reports on the simultaneous detection of triazole antifungal drugs and glycopeptide antibiotics by LC-MS / MS

Method used

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  • Kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics and detection method thereof
  • Kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics and detection method thereof
  • Kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics and detection method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Example 1 Kit for Simultaneous Detection of Triazole Antifungal Drugs and Glycopeptide Antibiotics

[0028] The present invention is a kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics, including calibration products, quality control products, diluents, extracts and mobile phases. The specific preparation methods of each component are as follows:

[0029] 1. Preparation of calibrator

[0030] Pipette 125 μL fluconazole stock solution (2 mg / mL), 25 μL voriconazole stock solution (2 mg / mL), 25 μL posaconazole stock solution (2 mg / mL), 25 μL itraconazole stock solution (2 mg / mL), Put 250μL of vancomycin stock solution (2mg / mL) and 250μL of teicoplanin stock solution (2mg / mL) in a 2mL sample bottle, then add 300μL of methanol into the sample bottle, and mix well to obtain a mixed standard mother solution; then pipette Put 200 μL of the mixed standard mother solution into a 1.5 mL centrifuge tube, add 800 μL of serum, mix well, and let ...

Embodiment 2

[0051] Example 2 The method for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics using the kit of Example 1 includes the following specific contents:

[0052] Take 50 μL of the calibrator and the sample to be tested (serum) in a 1.5 mL centrifuge tube; add 950 μL of the extract solution to the centrifuge tube, vortex and mix for 5 min, then centrifuge at 4 °C and 13000 rpm for 5 min, and take 100 μL of the supernatant in the sample In the bottle, add 100 μL diluent (i.e. distilled water or ultrapure water) to the sample bottle, mix well, detect with LC-MS / MS, record the peak area and The peak area of ​​the isotopic internal standard.

[0053] The mass spectrometry detection conditions of the LC-MS / MS in the detection of the present invention are as follows: the ion source is an electrospray ionization ion source (ESI, positive ion mode); the capillary voltage is 0.8kV; the desolvation gas temperature is 500°C; h; backflush flow rate 50L / h; desol...

Embodiment 3

[0065] Embodiment 3 Accuracy evaluation of the kit of the present invention

[0066] Detect quality control product 1 and quality control product 2 in embodiment 1 by the detection method in embodiment 2, then according to the ratio of the peak area of ​​each compound in the quality control product and the ratio of the isotopic internal standard, and the linear regression equation of each compound , calculate the concentration and recovery rate of each compound in the quality control product, and the specific data are shown in Table 8 below.

[0067] Table 8

[0068]

[0069] It can be seen from Table 8 that the recoveries of fluconazole, voriconazole, posaconazole, itraconazole, vancomycin and teicoplanin in quality control product 1 were 99.1%, 99.5%, 99.2%, and 99.2% in sequence , 99.5%, 99.6%, the recoveries of fluconazole, voriconazole, posaconazole, itraconazole, vancomycin and teicoplanin in quality control product 2 were 100.1%, 99.8%, 100.6%, 101.4%, 100.4%, 99.2...

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Abstract

The invention discloses a kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics, the kit comprises an extracting solution, a diluent, a mobile phase, a calibrator anda quality control material, the calibrator and the quality control material comprise fluconazole, voriconazole, posaconazole, itraconazole, vancomycin and teicoplanin; wherein the extracting solutioncomprises an isotope internal standard substance and a methanol aqueous solution, and the isotope internal standard substance comprises fluconazole-d4, voriconazole-d3, posaconazole-d4 and itraconazole-d5; wherein the diluent is distilled water or ultrapure water. The invention has the advantages that the invention firstly provides a kit capable of detecting a micromolecular antifungal drug and simultaneously detecting macromolecule glycopeptide antibiotics; the kit is simple in sample pretreatment and short in pretreatment time, improves the detection efficiency, can detect multiple types ofmedicines, can meet the detection requirements of clinically high-throughput samples, has a very good application prospect, and is convenient to popularize.

Description

technical field [0001] The invention relates to the field of clinical detection, in particular to a kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics, and also relates to a kit for simultaneously detecting triazole antifungal drugs and glycopeptide antibiotics in plasma and serum. approach to glycopeptide antibiotics. Background technique [0002] At present, invasive fungal infection has become the main factor leading to increased morbidity and mortality in severe patients. For example, the mortality rate of invasive lung disease caused by Aspergillus is as high as 82%. Clinically used antifungal drugs are mainly triazole antifungal drugs (belonging to small molecule drugs), mainly including fluconazole, voriconazole, posaconazole and itraconazole. In addition, methicillin-resistant Staphylococcus aureus (MRSA) is also a common and highly virulent bacterium in clinical practice. It has become one of the important pathogens of nosocomia...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/8672
Inventor 蔡克亚耿瑞刘亚娟赵高岭
Owner AUTOBIO DIAGNOSTICS CO LTD
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