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Method for identifying apis cerana honey and apis mellifera honey

A technology of honey and honey bees, applied in the direction of instruments, measuring devices, scientific instruments, etc., can solve the problems that do not conform to the development trend of efficient and rapid identification, restrict the healthy development of the Chinese bee industry, and reduce the content, so as to achieve health and safety The effects of sustainable development, promotion of industrialization development, and ease of operation and promotion

Inactive Publication Date: 2020-07-10
BEE RES INST CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the differences in production methods and quality between Chinese bee honey and Italian bee honey, there is a significant difference in price between the two. Therefore, in order to pursue high profits, some lawbreakers use Italian bee honey to pretend to be or mix it with Chinese bee honey. Well, it has caused serious economic losses to the vast number of Chinese beekeepers and related enterprises, and it has also restricted the healthy development of the Chinese bee industry
[0003] In recent years, the development of the identification technology of Chinese bee honey and Italian bee honey has been widely concerned by researchers and related bee product companies. The above technologies all rely on proteins as characteristic biomarkers for identification, but require complex sample pretreatment processes such as complex protein extraction, concentration determination, protein electrophoretic separation, and band identification, as well as complex MALDI-TOF-TOF technology, which is not in line with Efficient and rapid identification of development trends
Chinese patent application 20180008004.2 reports a method for identifying Chinese bee honey and Italian bee honey based on beeswax alkanes, but as a volatile component, its content will decrease significantly with the prolongation of storage time
In recent years, small molecular endogenous substances have been widely used in the identification of food authenticity and traceability due to their stable presence in the honey matrix and the convenience of extraction and determination. However, there is no relevant report on the identification of Chinese bee honey and Italian bee honey

Method used

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  • Method for identifying apis cerana honey and apis mellifera honey
  • Method for identifying apis cerana honey and apis mellifera honey
  • Method for identifying apis cerana honey and apis mellifera honey

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] (1) Construction of discriminant model:

[0048] Weigh 3g of honey samples (respectively Chinese honey samples (see Table 1 for specific information) and Italian honey samples (see Table 1 for specific information)) and add 5ml of 10% methanol aqueous solution to dilute, ultrasonically dissolve and extract honey for 10 minutes, 12000rpm , centrifuged at 4°C for 10 min, and separated by liquid chromatography tandem high-resolution mass spectrometry. The liquid chromatography instrument conditions are: Thermo Scientific Hypersil GOLD C18 (100×2.1mm, 1.9μm) chromatographic column, column temperature 40°C, injection volume 5μL, 0.1% formic acid water as mobile phase A, acetonitrile as mobile phase B, Flow rate 0.3mL / min, using gradient elution program: 0-2min, 5% mobile phase B; 7min, 30% mobile phase B; 13-18min, 95% mobile phase B; 18.1-20min, 5% mobile phase B. The conditions of the high-resolution mass spectrometer are: spray voltage 3.5kV(+), 3.2kV(-); spray temperat...

Embodiment 2

[0052] (1) Construction of discriminant model:

[0053] Weigh 2g of honey samples (respectively Chinese honey samples (see Table 1 for specific information) and Italian honey samples (see Table 1 for specific information)) and add 8ml of 8% methanol aqueous solution to dilute, ultrasonically dissolve and extract honey for 10 minutes, 10000rpm , centrifuged at 4°C for 10 min, and separated by liquid chromatography tandem high-resolution mass spectrometry. The liquid chromatography instrument conditions are: Thermo Scientific Hypersil GOLD C18 (100×2.1mm, 1.9μm) chromatographic column, column temperature 40°C, injection volume 5μL, 0.1% formic acid water as mobile phase A, acetonitrile as mobile phase B, Flow rate 0.3mL / min, using gradient elution program: 0-2min, 5% mobile phase B; 7min, 30% mobile phase B; 13-18min, 95% mobile phase B; 18.1-20min, 5% mobile phase B. The conditions of the high-resolution mass spectrometer are: spray voltage 3.5kV(+), 3.2kV(-); spray temperatu...

Embodiment 3

[0057] (1) Model construction:

[0058] Weigh 5g of honey samples (respectively Chinese honey samples (see Table 1 for specific information) and Italian honey samples (see Table 1 for specific information)) and add 30ml of 12% methanol aqueous solution to dilute, ultrasonically dissolve and extract honey for 10 minutes, 14000rpm , centrifuged at 4°C for 10 min, and separated by liquid chromatography tandem high-resolution mass spectrometry. The liquid chromatography instrument conditions are: Thermo Scientific Hypersil GOLD C18 (100×2.1mm, 1.9μm) chromatographic column, column temperature 40°C, injection volume 5μL, 0.1% formic acid water as mobile phase A, acetonitrile as mobile phase B, Flow rate 0.3mL / min, using gradient elution program: 0-2min, 5% mobile phase B; 7min, 30% mobile phase B; 13-18min, 95% mobile phase B; 18.1-20min, 5% mobile phase B. The conditions of the high-resolution mass spectrometer are: spray voltage 3.5kV(+), 3.2kV(-); spray temperature 350°C, shea...

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Abstract

The invention belongs to the field of food detection, and particularly relates to a method for identifying apis cerana honey and apis mellifera honey. The method comprises the steps of sample extraction, liquid chromatography-tandem high-resolution mass spectrometry detection and analysis. The provided method is a simple, quick and environment-friendly method for identifying apis cerana honey andapis mellifera honey. Compared with other methods, the method provided by the invention has the advantages that a feature region is completely visualized, the endogenous substance information is completely acquired, the operation and popularization are convenient, the important significance is realized on accurately identifying the apis cerana honey and the apis mellifera honey, the industrial development of bee products is favorably promoted, and the healthy and sustainable development of the beekeeping industry is favorably realized.

Description

technical field [0001] The invention relates to the field of food detection, in particular to a method for distinguishing Chinese bee honey and Italian bee honey. Background technique [0002] Honey is a natural sweet substance that bees collect from the nectar, secretion or honeydew of plants, combine with their own secretions, and fully brew. Different bee species, nectar source plants, origins, and even differences in harvesting and processing methods can lead to differences in endogenous active components in honey. Apis mellifera and Apis mellifera are the two main species of bees raised in my country. At present, there are about 9 million bee colonies in my country, of which 8 million groups of Apis mellifera and about 1 million groups of Apis mellifera. Italian bee honey is collected and brewed from Italian bees introduced from abroad. Italian bees are easy to raise, reproduce quickly, have high honey yield, and can collect honey from a large area. However, the produ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88G01N30/86G01N30/06
CPCG01N30/88G01N30/8675G01N30/8696G01N30/06G01N2030/8809
Inventor 周金慧王欣然李熠陈兰珍杨术鹏张金振金玥杨宇晖赵文王鹏黄京平
Owner BEE RES INST CHINESE ACAD OF AGRI SCI
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