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Determination of Taxin in Red Heart Duck Eggs by High Performance Liquid Chromatography Tandem Mass Spectrometry

A high-performance liquid chromatography and tandem mass spectrometry technology, which is applied to the field of high-performance liquid chromatography and tandem mass spectrometry determination of taxane in red-hearted duck eggs, can solve problems such as no reports, and achieves simple operation, good accuracy and large application. Effects of potential and economic value

Active Publication Date: 2022-04-01
南宁海关技术中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the detection and quantification method of taxane in natural red duck eggs

Method used

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  • Determination of Taxin in Red Heart Duck Eggs by High Performance Liquid Chromatography Tandem Mass Spectrometry
  • Determination of Taxin in Red Heart Duck Eggs by High Performance Liquid Chromatography Tandem Mass Spectrometry
  • Determination of Taxin in Red Heart Duck Eggs by High Performance Liquid Chromatography Tandem Mass Spectrometry

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A high-performance liquid chromatography tandem mass spectrometry method for determination of taxane in red-hearted duck eggs, the specific steps are:

[0036] I, standard solution preparation

[0037] Precisely weigh 10 mg of taxane standard substance into 100 mL volumetric flasks, dissolve with a small amount of dichloromethane, and then make up to the mark with acetonitrile, prepare a standard stock solution with a concentration of 100 μg / mL, and store at -20 °C. When used, dilute it with acetonitrile into a series of standard working solutions of appropriate concentration according to the specific situation;

[0038] II. Sample pretreatment

[0039] Accurately weigh 5.0 g (accurate to 0.01 g) of a homogeneous sample into a 50 mL centrifuge tube, add 20 mL of acetonitrile and 5 g of anhydrous sodium sulfate, vortex and mix for 1 min, ultrasonically extract for 15 min, and then centrifuge at 4000 r / min for 5 min. The supernatant was transferred to a 100 mL rotary ev...

Embodiment 2

[0051]The high-performance liquid chromatography tandem mass spectrometry method for the determination of taxane in red-hearted duck eggs is the same as in Example 1, except that the acetonitrile in step II is replaced with dichloromethane.

Embodiment 3

[0053] The high-performance liquid chromatography tandem mass spectrometry method for the determination of taxane in red-hearted duck eggs is the same as in Example 1, except that the acetonitrile in step II is replaced with acetone.

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Abstract

The invention discloses a high-performance liquid chromatography tandem mass spectrometry method for determining taxin in red duck eggs, and belongs to the technical field of analysis and detection. The present invention establishes a quantitative detection method for taxin in red-hearted duck eggs by utilizing high-performance liquid chromatography tandem mass spectrometry (LC-MS / MS). Among them, the red heart duck egg sample was extracted with acetonitrile, concentrated by rotary evaporation, degreased with n-hexane, and tested on a computer. The results show that the method is simple to operate, high in sensitivity and good in accuracy, and it provides a method reference for the determination of taxin in red duck eggs, and has great application potential and economic value in the fields of red duck egg processing, production and quality grading.

Description

technical field [0001] The invention belongs to the technical field of analysis and detection, and in particular relates to a high-performance liquid chromatography tandem mass spectrometry method for measuring taxane in red-hearted duck eggs. Background technique [0002] The yolk of the natural red-hearted duck egg is cinnabar red, with stable and natural color, and is very popular among consumers in the market. The yellow pigment of ordinary egg yolk is mainly composed of yellow carotenoids such as lutein, zeaxanthin, neoxanthin, and violaxanthin, and its source is the yellow carotenoids in cereal grains or green plants. According to relevant researches, laying ducks can produce natural red-hearted duck eggs by stocking or feeding fresh grass. The natural red-hearted duck eggs contain a red carotenoid called Rhodoxanthin, whose molecular formula is C 40 H 50 O 2 , see the structural formula figure 1 . Studies have shown that taxane has antioxidant, free radical scave...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/12
CPCG01N30/02G01N30/12G01N2030/126
Inventor 蔡翔宇欧玉玲梁宇
Owner 南宁海关技术中心