Method for simultaneously separating schaftoside, vichnine-2 and ecdysone and application

Abstract

The invention belongs to the technical field of phytochemistry. The invention discloses a method for simultaneously separating schaftoside, vichnine-2 and ecdysone and application. Elsholtzia rugulosais used as an extraction raw material, 90% ethanol is used as an extraction solvent, the extracting solution is concentrated under reduced pressure, and the extracting solution is extracted with petroleum ether, ethyl acetate and n-butyl alcohol in sequence; the concentrated n-butyl alcohol extraction part is coarsely separated by using AB-8 macroporous resin to obtain a 50% ethanol elution part;and ODS column chromatography separation is performed on the eluted part to obtain an ecdysone monomer, impurity-containing schaftoside and impurity-containing vichnine-2, and silica gel column chromatography separation and purification are respectively performed on the impurity-containing schaftoside and impurity-containing vichnine-2 to respectively obtain a schaftoside monomer and a vichnine-2monomer. The method is short in separation and purification process, simple to operate and easy to implement, and plant resources and extraction and separation cost are greatly saved; and great economic benefits can be generated, and the development of industries in related fields can be promoted.

Description

technical field [0001] The invention belongs to the technical field of phytochemistry, and in particular relates to a method and application for simultaneously isolating schiaftoside, vitsanin-2 and ecdysone. Background technique [0002] At present, schaftoside is a flavonoid component extracted from Desmodium sativa, and it is used as an index component for the quality inspection of S. sativa. Schaftoside has choleretic, antibacterial, and anti-inflammatory effects. Schaftosides are mainly used clinically for diseases such as hepatitis, cholecystitis, gallstones, and urinary system infections. A study explored the protective effect of schiaftoside on high-fat diet-induced non-alcoholic fatty liver disease (NAFLD) in mice. Ecdysone acts on the human body to promote the synthesis of collagen protein, eliminate cholesterol in the body, lower blood lipids, inhibit blood sugar rise, anti-arrhythmia, dredge meridians, activate collaterals, dehumidify and relieve pain, etc. It i...

AI Technical Summary

Problems solved by technology

[0006] Through the above analysis, the existing problems and defects of the prior art are: there is no patent report on the comprehensive extraction and separation of these three components from Gucao lacquer. The three-substance approach of schaftoside, vitzenin-2 and ecdysone,

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