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Research of molecular mechanism of IGF-1 in skeletal muscle damage repair

A technology of IGF-1 and growth factor, applied in medical preparations containing active ingredients, muscular system diseases, organic active ingredients, etc., can solve the problem of lack of insulin-like growth factor-1, etc., to promote the differentiation and maturation of skeletal muscle , the effect of promoting proliferation and differentiation, and promoting angiogenesis

Pending Publication Date: 2020-07-31
SHANDONG PROVINCIAL HOSPITAL
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, there is no research on the molecular mechanism of insulin-like growth fac

Method used

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  • Research of molecular mechanism of IGF-1 in skeletal muscle damage repair
  • Research of molecular mechanism of IGF-1 in skeletal muscle damage repair
  • Research of molecular mechanism of IGF-1 in skeletal muscle damage repair

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Example 1 Mb IGF-1 Study on cell proliferation and pathway mechanism

[0041] Methods: Two-day-old male Lewis rat limb muscles were isolated and cultured in vitro to isolate and culture myoblasts (Mb), and the third generation myoblasts (Mb) were transfected with recombinant adenovirus carrying IGF-1 gene. IGF-1 ), and Mb and Mb IGF-1 The cell scaffold complex was constructed by co-culture with the decellularized collagen sponge scaffold in vitro, and the cell adhesion was observed after 72 hours; DMSO was added to the scaffold complex and shaken for 20 minutes, and the cell proliferation was detected by the MTT method; cells at 0, 24, 48, and 72 hours MRNA levels of mTOR, p70S6K, ERK1, and Foxo3a genes.

[0042] result:

[0043] 1. The decellularized collagen sponge scaffold material has a loose and porous three-dimensional structure, compounded with Mb and Mb IGF-1 After incubation, the volume swells, resembling a gel, with a certain volume and elasticity, similar to the...

Embodiment 2

[0046] Example 2 Mb IGF-1 Differentiation

[0047] Methods: Two-day-old male Lewis rat limb muscles were isolated and cultured in vitro to isolate and culture myoblasts (Mb), and the third generation myoblasts (Mb) were transfected with recombinant adenovirus carrying IGF-1 gene. IGF-1 ). Undifferentiated cells were cultured in 10% FBS high-sugar DMEM medium. When the cells grew to 60%-70% confluence, they were replaced with 2% horse blood DMEM medium to induce myogenic differentiation. Cells were collected at 3d, 6d and 9d, and the expression of mRNA levels of related differentiation marker genes (MyoG and MyHC) and IGF-1 gene were determined.

[0048] Result: The result is Picture 12 , Figure 13 with Figure 14 As shown, with Mb IGF-1 The expression of IGF-1 gene increased significantly, and its expression change trend was consistent with the myogenic differentiation marker genes MyoG and MyHC; Figure 15 , Figure 16 with Figure 17 As shown, compared with Mb differentiatio...

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Abstract

The invention relates to IGF-1 which can promote proliferation and differentiation of myoblasts, and an Akt/mTOR pathway and an ERK pathway participate in the promotion effect of overexpression of theIGF-1 on proliferation and differentiation of the myoblasts in skeletal muscle tissue engineering. The effect of promoting proliferation and differentiation of the myoblasts by the IGF-1 under the participation of the Akt/mTOR pathway is jointly completed by two ways of enhancing the cell synthesis effect by phosphorylation and activation of p70S6K and enhancing the anti-cell-decomposition effectby phosphorylation and activation of Foxo3a. The discovery provides a new idea for constructing more mature tissue-engineered skeletal muscle tissues in the future so as to finally realize functionalreduction of tongue defects.

Description

Technical field [0001] The present disclosure relates to the study of the molecular mechanism of IGF-1 in the repair of skeletal muscle injury. Background technique [0002] Disclosure of the background information is only intended to increase some understanding of the overall background of the present disclosure, and is not necessarily regarded as an acknowledgement or any form of suggestion that the information constitutes the prior art known to those of ordinary skill in the art. [0003] Skeletal muscle defects or atrophy caused by various reasons such as trauma, tumors, metabolic diseases, etc. are very common. The current treatment is mainly through muscle transplantation for morphological and functional replacement. However, the available muscles of this method are limited and the supply is limited. The zone causes great damage, thus restricting its application. The ideal method should be prefabricated in vitro with a certain shape and functional skeletal muscle implanted i...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K31/7088A61P21/00
CPCA61K45/00A61K31/7088A61P21/00
Inventor 周伟杜鹃白金博于连聘张卫东
Owner SHANDONG PROVINCIAL HOSPITAL
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