Storage method of adipose-derived stem cells

An adipose stem cell and storage method technology, applied in the preservation, application, animal husbandry and other directions of human or animal body, can solve the problems of low cell viability, cell oxidative damage, etc., to expand blood volume, improve viability, and eliminate blood vessels. Efficacy of endoerythrocyte aggregation and thrombosis

Inactive Publication Date: 2020-08-04
深圳安吉赛尔生物科技集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Cell cryopreservation is one of the main methods of cell preservation. The current cell cryopreser

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for storing fat stem cells, the specific steps are as follows:

[0023] (1) separating and culturing adipose stem cells from adipose tissue;

[0024] (2) Add the fat stem cells described in step (1) into the cell cryopreservation tube equipped with the fat stem cell storage solution, and seal the mouth of the tube; the concentration of the fat stem cells is 1×10 6 a / mL;

[0025] Wherein, every 100mL of said adipose stem cell storage solution consists of the following components and contents:

[0026] 2g carboxylated poly-L-lysine, 5g trehalose, 1.5mL DMSO, 8mL glycerol, 25mL serum-free medium, 1mL low molecular weight dextran, 3g glucose, penicillin 100U / mL, streptomycin 100μg / mL, The amount is PBS buffer solution;

[0027] (3) Freezing the sealed cell cryopreservation tube described in step (2).

Embodiment 2

[0029] A method for storing fat stem cells, the specific steps are as follows:

[0030] (1) separating and culturing adipose stem cells from adipose tissue;

[0031] (2) Add the adipose stem cells described in step (1) into the cell cryopreservation tube equipped with the adipose stem cell storage solution, and seal the mouth of the tube; the concentration of the adipose stem cells is 0.5×10 6 a / mL;

[0032] Wherein, every 100mL of said adipose stem cell storage solution consists of the following components and contents:

[0033] 0.6g carboxylated poly-L-lysine, 1g trehalose, 1mL DMSO, 6mL glycerol, 20mL serum-free medium, 0.2mL low molecular weight dextran, 2g glucose, penicillin 80U / mL, streptomycin 75μg / mL, The balance is PBS buffer solution;

[0034] (3) Freezing the sealed cell cryopreservation tube described in step (2).

Embodiment 3

[0036] A method for storing fat stem cells, the specific steps are as follows:

[0037] (1) separating and culturing adipose stem cells from adipose tissue;

[0038] (2) Add the adipose stem cells described in step (1) into the cell cryopreservation tube equipped with the adipose stem cell storage solution, and seal the mouth of the tube; the concentration of the adipose stem cells is 2×10 6 a / mL;

[0039] Wherein, every 100mL of said adipose stem cell storage solution consists of the following components and contents:

[0040] 4g carboxylated poly-L-lysine, 10g trehalose, 3mL DMSO, 10mL glycerol, 30mL serum-free medium, 2mL low molecular weight dextran, 5g glucose, penicillin 90U / mL, streptomycin 85μg / mL, balance For HEPES buffer;

[0041] (3) Freezing the sealed cell cryopreservation tube described in step (2).

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PUM

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Abstract

The invention discloses a storage method of adipose-derived stem cells. The storage method comprises the following steps: separating adipose-derived stem cells from adipose tissues and culturing the adipose-derived stem cells; adding the adipose-derived stem cells into a cell cryopreservation tube filled with an adipose-derived stem cell storage solution, and sealing a tube opening; and freezing the sealed cell cryopreservation tube, wherein every 100mL of the adipose-derived stem cell storage liquid is prepared from the following components: 0.6-4g of carboxylated poly-L-lysine, 1-10g of trehalose, 1-3mL of DMSO, 6-10mL of glycerin, 20-30mL of a serum-free medium, 0.2-2mL of low-molecular dextran, 2-5g of glucose, 80-100U/mL of penicillin, 75-100 [mu]g/mL of streptomycin, and the balanceof a buffer solution. According to the storage method of adipose-derived stem cells, the survival rate of the resuscitated adipose-derived stem cells can be remarkably increased.

Description

technical field [0001] The invention relates to the field of biotechnology, and more specifically relates to a method for storing fat stem cells. Background technique [0002] Adipose-derived stem cells (ADSCs) are a kind of stem cells with multi-directional differentiation potential isolated from adipose tissue in recent years. Adipocytes, chondrocytes, muscle cells, osteoblasts, nerve cells, glial cells and islet cells differentiate, and can secrete a variety of pro-angiogenic and anti-apoptotic factors. [0003] Adipose stem cells are a type of mesenchymal stem cells that exist in adipose tissue, accounting for about 10-20% of the total cells in subcutaneous white adipose tissue. Compared with stem cells from other sources, ADSCs have great advantages in isolation and culture: (1) Convenient sampling: adipose tissue is rich in reserves, and the sampling is less painful; currently, one liposuction can obtain 200mL of fat, and about 1×10 6 Stem cells, 40 times the amount ...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0221A01N1/0226
Inventor 孔丹青
Owner 深圳安吉赛尔生物科技集团有限公司
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