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Application of a kind of β-myrcene in drought resistance and growth promotion of alfalfa
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A technology of alfalfa and myrcene, applied in the fields of application, lawn growth, plant growth regulator, etc., to achieve significant effects of drought resistance and growth promotion
Active Publication Date: 2022-02-11
XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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[0016] At present, the research on the growth-promoting and drought-resistant effects of β-myrcene on plants is still in a blank stage at home and abroad.
Method used
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Embodiment 1
[0041] Growth-promoting effect of β-myrcene on alfalfa seedlings in petri dishes:
[0042] β-myrcene was prepared with Tween 20 to a concentration of 10 μl / mL, and then diluted to 5 μl / mL, 2 μl / mL, 1 μl / mL, 0.5 μl / mL and 0.25 μl / mL;
[0043] Place filter paper in a petri dish with a diameter of 9 cm and add 60 alfalfa seeds, then add 5 ml of β- Myrcene solution, each treatment was repeated twice, and used as a blank control of distilled water; sealed with a parafilm, placed in a 25°C incubator for 5 days in the dark, and then measured the seedling height and root length of the seedlings;
[0044] Data statistics method: first calculate the growth rate of the root length and seedling height of alfalfa under each concentration to the blank control: Then use one-way ANOVA to detect whether the difference between the data of each group of the growth rate is significant, and then use the LSD method to analyze the data, and the difference between the groups with different marked l...
Embodiment 2
[0047] Growth-promoting effects of β-myrcene on potted alfalfa under normal and drought-treated conditions:
[0048] Prepare β-myrcene with Tween 20 into 0.1 μl / mL and 0.25 μl / mL solutions, put the solution in a small watering can and mark it, and add distilled water to another small watering can as a blank control;
[0049] When the alfalfa seedlings in the pot grow to about 10cm, start spraying the prepared solutions with concentrations of 0.1μl / mL and 0.25μl / mL, spray once every 3 days, spray 10 times each time, and set 2 for each concentration. A spraying method, that is, spraying 2 times and spraying 4 times, and the potted plants were divided into two groups, one group was watered normally, and the other group was watered in half, and the watering frequency was halved as a drought treatment. After spraying the solution for 20 days, Measure the root length, seedling height and fresh weight of alfalfa, and measure the dry weight after putting fresh alfalfa in an oven for d...
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Abstract
The invention discloses an application of β-myrcene in drought resistance and growth promotion of alfalfa. β-myrcene is prepared into a low-concentration aqueous solution with Tween 20, and alfalfa seeds are cultivated in a petri dish. The results show that β-myrcene significantly Promoted root growth of alfalfa seedlings. β‑myrcene was selected to prepare an aqueous solution with Tween 20, and sprayed on alfalfa seedlings under normal and drought conditions. Alfalfa has a more significant promoting effect. It can be seen that β‑myrcene has obvious drought resistance and growth promoting effect on alfalfa. β-myrcene is a natural product that can be degraded in nature, is non-toxic and harmless to the environment, and can be prepared in large quantities, with low cost, low concentration, small dosage, strong effect, and high application potential .
Description
technical field [0001] The invention relates to the application of a compound to the drought-resistant and growth-promoting effect of grass, in particular to the application of β-myrcene in the drought-resistant and growth-promoting effect of alfalfa. Background technique [0002] Alfalfa is an important herbage, and the state has listed the "industrialization of alfalfa" as a scientific and technological project, in order to realize the optimization of my country's planting industry structure and promote the sustainable development of agriculture. As a high-quality variety of alfalfa, alfalfa is an important source of high-quality protein in animal husbandry. Alfalfa is suitable for warm and semi-humid to semi-arid climates, and is mostly distributed in the north of the Yangtze River in my country. In areas with less precipitation, alfalfa is facing the problem of how to resist drought and increase production. The traditional method mainly relies on coupling water and fert...
Claims
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Patent Type & Authority Patents(China)
IPC IPC(8): A01N27/00A01P21/00A01G7/06A01G20/00
CPCA01N27/00A01G7/06A01G20/00
Inventor 邵华石凯姜春宇黄岭周世兴张弛韩彩霞
Owner XINJIANG INST OF ECOLOGY & GEOGRAPHY CHINESE ACAD OF SCI
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