Application of a kind of β-myrcene in drought resistance and growth promotion of alfalfa
A technology of alfalfa and myrcene, applied in the fields of application, lawn growth, plant growth regulator, etc., to achieve significant effects of drought resistance and growth promotion
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Embodiment 1
[0041] Growth-promoting effect of β-myrcene on alfalfa seedlings in petri dishes:
[0042] β-myrcene was prepared with Tween 20 to a concentration of 10 μl / mL, and then diluted to 5 μl / mL, 2 μl / mL, 1 μl / mL, 0.5 μl / mL and 0.25 μl / mL;
[0043] Place filter paper in a petri dish with a diameter of 9 cm and add 60 alfalfa seeds, then add 5 ml of β- Myrcene solution, each treatment was repeated twice, and used as a blank control of distilled water; sealed with a parafilm, placed in a 25°C incubator for 5 days in the dark, and then measured the seedling height and root length of the seedlings;
[0044] Data statistics method: first calculate the growth rate of the root length and seedling height of alfalfa under each concentration to the blank control: Then use one-way ANOVA to detect whether the difference between the data of each group of the growth rate is significant, and then use the LSD method to analyze the data, and the difference between the groups with different marked l...
Embodiment 2
[0047] Growth-promoting effects of β-myrcene on potted alfalfa under normal and drought-treated conditions:
[0048] Prepare β-myrcene with Tween 20 into 0.1 μl / mL and 0.25 μl / mL solutions, put the solution in a small watering can and mark it, and add distilled water to another small watering can as a blank control;
[0049] When the alfalfa seedlings in the pot grow to about 10cm, start spraying the prepared solutions with concentrations of 0.1μl / mL and 0.25μl / mL, spray once every 3 days, spray 10 times each time, and set 2 for each concentration. A spraying method, that is, spraying 2 times and spraying 4 times, and the potted plants were divided into two groups, one group was watered normally, and the other group was watered in half, and the watering frequency was halved as a drought treatment. After spraying the solution for 20 days, Measure the root length, seedling height and fresh weight of alfalfa, and measure the dry weight after putting fresh alfalfa in an oven for d...
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