Paenibacillus amyloliquefaciens ky15, bacterial agent, application and products using it

A technology of bacillus and amylolysis, applied in the field of microorganisms, can solve the problems of single function and increase of production cost, and achieve the effect of alleviating the abuse of chemical fertilizers and promoting the growth of plant roots

Active Publication Date: 2020-07-28
康生元(肇庆)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional microorganisms used for soil remediation and improvement, crop quality and efficiency improvement, and weight loss and efficiency enhancement usually have a single function, and often require a variety of microorganisms to be used together, which increases production costs

Method used

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  • Paenibacillus amyloliquefaciens ky15, bacterial agent, application and products using it
  • Paenibacillus amyloliquefaciens ky15, bacterial agent, application and products using it
  • Paenibacillus amyloliquefaciens ky15, bacterial agent, application and products using it

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Experimental program
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preparation example Construction

[0049] In the preparation of the fertilizer according to the present invention, optionally, the bacterial strain KY15 or the bacterial agent containing it can be directly mixed with other components to prepare a fertilizer containing the bacterial strain KY15; alternatively, the bacterial strain KY15 or the bacterial agent containing it can be mixed The agent is first prepared as a fertilizer additive, which is applied to crops or soil after being mixed with other conventional fertilizers or pre-containing bacterial strain KY15 in the actual production process, so as to facilitate the adjustment of the dosage of bacterial strain KY15 in the fertilizer; optionally, the The bacterial strain KY15 or the microbial agent containing it is firstly prepared as a fertilizer additive, which is not mixed with the fertilizer and applied separately.

[0050] Based on the above application, the present invention also provides a fertilizer, which contains the bacterial strain KY15 or the bact...

Embodiment 1

[0058] Screening microbial strains with potassium decomposing function:

[0059] The first step of enrichment: Take 1g of soil sample and shake it in 10mL of pure water, take 100μL of it and mix it in potassium liquid medium (yeast powder 0.5g, glucose 10g, disodium hydrogen phosphate 2g, ammonium sulfate 1g, magnesium sulfate heptahydrate 0.5g, calcium carbonate 1g, potassium feldspar 1g, water 1L), incubate at 30°C for 3 days with shaking at 200r / min, take the bacterial solution and dilute 10 -6 、10 -7 , and 10 -8After doubling, smear it on the solid selection medium for dissolving potassium (yeast powder 0.5g, glucose 10g, disodium hydrogen phosphate 2g, ammonium sulfate 1g, magnesium sulfate heptahydrate 0.5g, calcium carbonate 1g, agar powder 15g, potassium feldspar 1g , water 1L), pick the functional strains.

[0060] The second step of enrichment: Take 100 μL of the bacterial solution enriched in the previous step in K liquid medium (yeast powder 0.5g, glucose 10g, d...

Embodiment 2

[0063] Collection of soil samples and screening of microbial strains with potassium decomposing function: representative soils with location characteristics collected from different provinces, cities, districts, and counties across the country, such as meadow soil, mountain forest soil, field soil, mud flow sand soil, etc. The target sample was screened for microbial strains with potassium decomposing function according to the enrichment method provided in Implementation 1. As a result, more than 200 microbial strains with strong potassium decomposing function were obtained, among which bacterial strain KY15, bacterial strain KY59, and bacterial strain KY107 had extremely strong decomposing Potassium function, results such as figure 1 shown. After further testing with different potassium-dissolving medium, the results showed that the potassium-dissolving hydrolytic circle of the strain KY15 was very stable.

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Abstract

The invention provides Paenibacillus amyloliquefaciens KY15, bacterial agent, application and products thereof, and relates to the field of microorganisms. The preservation number of Paenibacillus amylolyticus KY15 in the General Microorganism Center of China Committee for Culture Collection of Microorganisms is CGMCC NO.17851. The strain has the ability to decompose potassium, silicon, phosphorus and cellulose at the same time, and Paenibacillus amyloliquefaciens KY15 has the ability to decompose potassium, silicon, organic phosphorus and The ability of inorganic phosphorus, cellulolytic ability and the growth promotion effect on corn roots are obviously superior to these two commercial strains. Paenibacillus amyloliquefaciens KY15 also has the ability to promote plant root development, which alleviates the existing problems The problem of lacking a strain with multiple functions.

Description

technical field [0001] The invention relates to the field of microbes, in particular to Paenibacillus amyloliquefaciens KY15, bacterial agent, application and products using the same. Background technique [0002] The average amount of chemical fertilizer used per mu of crops in my country is 21.9 kg, much higher than the world average (8 kg per mu), 2.6 times that of the United States and 2.5 times that of the European Union. China has 2 billion mu of arable land and a population of 1.4 billion. Although it is a large agricultural country, it is also a populous country. In order to ensure food supply, it is endlessly asking for food from arable land. The long-term abuse of chemical fertilizers has caused serious ecological and environmental consequences and seriously affected my country's agriculture. of sustainable development. At present, countries all over the world are actively seeking better solutions. [0003] Microorganisms are the national demand and key research a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C05F11/08A01N63/22A01P21/00C09K17/14C09K101/00C12R1/01
CPCA01N63/00C05F11/08C09K17/14C09K2101/00C12N1/20C12N1/205C12R2001/01
Inventor 康耀卫王德江
Owner 康生元(肇庆)生物科技有限公司
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