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A method for promoting the growth of plant lactobacillus st-iii

A technology of ST-III, Lactobacillus plantarum, applied in the field of microbial fermentation, to achieve obvious growth promotion effect

Active Publication Date: 2020-11-03
BRIGHT DAIRY & FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although the above studies have made some technological breakthroughs, they generally achieve the growth of Lactobacillus plantarum by adding additional, growth-promoting ingredients to the milk medium, which has great limitations in theoretical research and practical production applications.

Method used

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  • A method for promoting the growth of plant lactobacillus st-iii
  • A method for promoting the growth of plant lactobacillus st-iii
  • A method for promoting the growth of plant lactobacillus st-iii

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Raw material preparation: raw milk, made up of 10% whole milk powder or skimmed milk powder, 5% sucrose and 100% water, after fully dissolving, mixing uniformly, homogeneous (70℃, 22MPa), 95℃ sterilization 10min, cool to 45℃ for later use.

[0035] Inoculate the raw milk separately with Lactobacillus plantarum ST-III (10 7 CFU / mL) and Leuconostoc mesenteroides BD01710 (3×10 6 CFU / mL), stir and mix evenly and then culture, the culture temperature is 37°C, the culture time is 24h, and the fermented milk sample is obtained.

[0036] The above fermented milk samples were sampled and counted by the plate counting method after gradient dilution. Compared with only inoculating Lactobacillus plantarum ST-III under the same conditions, the results are shown in Table 1. It can be seen that the content of active Lactobacillus plantarum ST-III in the fermented milk is higher than 5×10 8 CFU / mL.

[0037] Table 1: The pH value and the number of viable bacteria of the fermented milk sample ...

Embodiment 2

[0040] Raw material preparation: raw milk, made up of 10% whole milk powder or skimmed milk powder, 5% sucrose and 100% water, after fully dissolving, mixing uniformly, homogeneous (70℃, 22MPa), 95℃ sterilization 10min, cool to 45℃ for later use.

[0041] Inoculate the raw milk with Lactobacillus plantarum ST-III (10 7 CFU / mL) and Leuconostoc mesenteroides BD01710 (10 6 CFU / mL), stir and mix evenly and then culture, the culture temperature is 40°C, the culture time is 26h, and the fermented milk sample is obtained.

[0042] The above fermented milk samples were sampled and counted by the plate counting method after gradient dilution. Compared with only inoculating Lactobacillus plantarum ST-III under the same conditions, the results obtained are shown in Table 2. It can be seen that the content of active Lactobacillus plantarum ST-III in the fermented milk is higher than 5×10 8 CFU / mL.

[0043] Table 2: The pH value and the number of viable bacteria of the fermented milk sample of E...

Embodiment 3

[0046] Raw material preparation: The culture medium is raw milk, which is made up of 10% whole milk powder or skim milk powder, 5% sucrose and 100% water, after being fully dissolved, mixed evenly, and homogeneous (60°C, 22MPa), Sterilize at 95°C for 5min, then cool to 40°C for later use.

[0047] Inoculate the raw milk with Lactobacillus plantarum ST-III (10 6 CFU / mL) and Leuconostoc mesenteroides BD01710 (10 7 CFU / mL), stir and mix evenly and then culture, the culture temperature is 35°C, the culture time is 18h, and the fermented milk sample is obtained.

[0048] The above fermented milk samples were sampled and counted by the plate counting method after gradient dilution. Compared with only inoculating Lactobacillus plantarum ST-III under the same conditions, the results are shown in Table 3. The content of active Lactobacillus plantarum in the fermented milk is higher than 5×10 8 CFU / mL.

[0049] Table 3: The pH value and the number of viable bacteria of the fermented milk samp...

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Abstract

The invention discloses a method for promoting the growth of Lactobacillus plantarum ST‑III. The method comprises the following steps: respectively inoculating Lactobacillus plantarum ST‑III and Leuconostoc enterococcus BD01710 into a culture medium, stirring and mixing evenly, and then cultivating, The cultivation temperature is 35-40°C, and the cultivation time is 18-26h. The positive and progressive effect of the present invention is that the present invention discloses a method for promoting the growth of Lactobacillus plantarum ST‑III based on the research results of the microbiome analysis in traditional fermented foods and the interaction relationship between microorganisms. Specifically, the present invention has discovered the growth-promoting effect of Leuconostoc enterocoliticus BD01710 on Lactobacillus plantarum through co-cultivation experiments, and based on this, a binary fermentation system based on Leuconostoc enterococcus entericoligos and Lactobacillus plantarum has been constructed. The system can realize the growth of Lactobacillus plantarum ST‑III.

Description

Technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for promoting the growth of Lactobacillus plantarum ST-III. Background technique [0002] Lactobacillus plantarum (Lactobacillus plantarum) is an important type of probiotics. It has become a hot spot in the development of functional probiotics due to its various physiological functions such as lowering cholesterol, lowering blood sugar, inhibiting pathogenic bacteria and enriching heavy metals. Lactobacillus plantarum ST-III is a probiotic with excellent performance. Because of its wide physiological activity, it has been added to various products. However, the large-scale application of Lactobacillus plantarum in the field of dairy products still faces a major technical bottleneck. The currently identified Lactobacillus plantarum strains are difficult to grow in many natural food media. [0003] In order to solve the above problems, the academic and business c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A23C9/123C12R1/25C12R1/01
CPCA23C9/1234C12N1/20A23V2400/161A23V2400/169
Inventor 鄢明辉韩瑨游春苹刘振民吴正钧
Owner BRIGHT DAIRY & FOOD CO LTD
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