A method for promoting the growth of plant lactobacillus st-iii
A technology of ST-III, Lactobacillus plantarum, applied in the field of microbial fermentation, to achieve obvious growth promotion effect
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Embodiment 1
[0034] Raw material preparation: raw milk, made up of 10% whole milk powder or skimmed milk powder, 5% sucrose and 100% water, after fully dissolving, mixing uniformly, homogeneous (70℃, 22MPa), 95℃ sterilization 10min, cool to 45℃ for later use.
[0035] Inoculate the raw milk separately with Lactobacillus plantarum ST-III (10 7 CFU / mL) and Leuconostoc mesenteroides BD01710 (3×10 6 CFU / mL), stir and mix evenly and then culture, the culture temperature is 37°C, the culture time is 24h, and the fermented milk sample is obtained.
[0036] The above fermented milk samples were sampled and counted by the plate counting method after gradient dilution. Compared with only inoculating Lactobacillus plantarum ST-III under the same conditions, the results are shown in Table 1. It can be seen that the content of active Lactobacillus plantarum ST-III in the fermented milk is higher than 5×10 8 CFU / mL.
[0037] Table 1: The pH value and the number of viable bacteria of the fermented milk sample ...
Embodiment 2
[0040] Raw material preparation: raw milk, made up of 10% whole milk powder or skimmed milk powder, 5% sucrose and 100% water, after fully dissolving, mixing uniformly, homogeneous (70℃, 22MPa), 95℃ sterilization 10min, cool to 45℃ for later use.
[0041] Inoculate the raw milk with Lactobacillus plantarum ST-III (10 7 CFU / mL) and Leuconostoc mesenteroides BD01710 (10 6 CFU / mL), stir and mix evenly and then culture, the culture temperature is 40°C, the culture time is 26h, and the fermented milk sample is obtained.
[0042] The above fermented milk samples were sampled and counted by the plate counting method after gradient dilution. Compared with only inoculating Lactobacillus plantarum ST-III under the same conditions, the results obtained are shown in Table 2. It can be seen that the content of active Lactobacillus plantarum ST-III in the fermented milk is higher than 5×10 8 CFU / mL.
[0043] Table 2: The pH value and the number of viable bacteria of the fermented milk sample of E...
Embodiment 3
[0046] Raw material preparation: The culture medium is raw milk, which is made up of 10% whole milk powder or skim milk powder, 5% sucrose and 100% water, after being fully dissolved, mixed evenly, and homogeneous (60°C, 22MPa), Sterilize at 95°C for 5min, then cool to 40°C for later use.
[0047] Inoculate the raw milk with Lactobacillus plantarum ST-III (10 6 CFU / mL) and Leuconostoc mesenteroides BD01710 (10 7 CFU / mL), stir and mix evenly and then culture, the culture temperature is 35°C, the culture time is 18h, and the fermented milk sample is obtained.
[0048] The above fermented milk samples were sampled and counted by the plate counting method after gradient dilution. Compared with only inoculating Lactobacillus plantarum ST-III under the same conditions, the results are shown in Table 3. The content of active Lactobacillus plantarum in the fermented milk is higher than 5×10 8 CFU / mL.
[0049] Table 3: The pH value and the number of viable bacteria of the fermented milk samp...
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