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Macrobrachium rosenbergii thymosin β4 gene, protein, preparation method and application thereof

A technology of Macrobrachium rosenbergii and thymosin, which is applied in the preparation methods of peptides, thymosin, peptide/protein components, etc., to achieve the effect of obvious antibacterial activity

Active Publication Date: 2022-06-14
YANGZHOU UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the above-mentioned technical problems, the present invention provides a recombinant expression preparation method and application of Macrobrachium rosenbergii thymosin β4 protein. , the problem of low yield

Method used

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  • Macrobrachium rosenbergii thymosin β4 gene, protein, preparation method and application thereof
  • Macrobrachium rosenbergii thymosin β4 gene, protein, preparation method and application thereof

Examples

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Embodiment 1

[0036] 1. Transformation of MrThyβ4-pET30A recombinant plasmid:

[0037] Take competent cells E.coli BL21 and put them in ice until they melt. Add the recombinant plasmid to the competent cells, mix the contents gently, and let stand in an ice bath for 3 minutes. Place the centrifuge tube in a 42°C water bath for 90 seconds, quickly transfer to an ice bath, and cool for 2-3 minutes without shaking. Add 900 μL of sterile LB medium (without antibiotics) to each centrifuge tube, mix well, and place on a shaker at 37°C for 45 minutes (15 rpm / min) to revive the bacteria. Place the recovered bacterial solution in a centrifuge at 1000rpm, centrifuge for 3min, discard 850μL supernatant, mix well, draw competent cells and add them to the LB solid medium containing the corresponding antibiotics, use a sterile elbow glass rod Gently spread the cells evenly, place at room temperature until the liquid is absorbed, invert the plate, and incubate at 37°C for 12-16h.

[0038] 2. Induced ex...

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Abstract

The invention belongs to the field of genetic engineering of molecular biology, and relates to the nucleotide sequence and encoded amino acid sequence of macrobrachium rosenbergii thymosin β4 gene, as well as the codon-optimized nucleotide sequence, in particular to macrobrachium rosenbergii thymosin Preparation method for recombinant expression of β4 protein. The recombinant expression of MrThyβ4 protein can overcome the problem of low yield in the purification and separation process, further study the function of the protein, and lay the foundation for the wide application of the protein in the research fields of biology, medicine and agriculture.

Description

technical field [0001] The invention belongs to the field of genetic engineering of molecular biology, and relates to the nucleotide sequence and the coded amino acid sequence of the thymosin β4 gene of Macrobrachium rosenbergii (Macrobrachium rosenbergii) and the nucleotide sequence after codon optimization, in particular to Macrobrachium rosenbergii (Macrobrachium rosenbergii) rosenbergii) Preparation method for recombinant expression of thymosin β4 protein. Background technique [0002] Thymosin widely exists in vertebrates and invertebrates, and can be divided into three types according to the different isoelectric points: Tα, Tβ, and Tγ. The functional domain of thymosin is a thymosin (THY) domain, and the β-thymosin protein in invertebrates usually has multiple domains. At present, thymosin participates in various biological functions of the body, including regulating actin and remodeling the cytoskeleton. In addition, β-thymosin protein can also play an important ro...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K14/575C12N15/16C12N15/70C07K1/22A61K38/22A61P31/04
CPCC07K14/57581C12N15/70A61P31/04A61K38/00C12N2800/22
Inventor 杨辉熊浩然付立霞张莹莹
Owner YANGZHOU UNIV
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