Composite bacterial powder, composite microbial agent thereof, and preparation method and application of composite microbial agent

A technology of composite microbial bacterial agent and composite bacterial powder, which is applied in the field of pesticides, can solve the problems of limited improvement effect, unstable effect, and insect drug resistance, and achieve the effect of excellent performance indicators

Inactive Publication Date: 2020-08-11
SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the inventors have found that the use of a single biocontrol strain to prevent and control pests has shortcomings such as unstable effects and narrow control spectrum, and in order to improve this shortcoming, the prior art mostly uses the combination of microorganisms and chemical agents or traditional Chinese medicines Prevention and control of pests and diseases, but the improvement effect is limited, and the use of chemical agents will still cause problems such as pesticide residues, environmental pollution and insect resistance

Method used

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  • Composite bacterial powder, composite microbial agent thereof, and preparation method and application of composite microbial agent
  • Composite bacterial powder, composite microbial agent thereof, and preparation method and application of composite microbial agent

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0046] Example 1 The preparation of spore powder is as follows:

[0047] The preparation method of described rose fume-colored Isosporium spore powder comprises the following steps:

[0048] Step 1: Cultivate slant strains, inoculate the original strains on the PDA slant, and put the inoculated slant culture medium into a 28°C incubator for 14 days to obtain the first batch of slant strains.

[0049] Step 2: Secondary liquid culture, each 1000ml liquid medium contains: 0.5g MgS0 4 ·7H 2 O, 0.5g KCl, 1.0g KH 2 P0 4 , 0.1g FeS0 4 ·7H 2 O, 40-45g sucrose, 5g peptone, and distilled water. Put the sterilized liquid culture medium into a 3000ml Erlenmeyer flask, fill each bottle with 700-800ml liquid culture medium, and inoculate one slant strain prepared in step 1 in each bottle under aseptic conditions. The liquid culture medium is placed on a shaker and shaken for 24-48 hours, the temperature is controlled at 25° C. to 28° C., and the rotation speed is 150-180 rad / min to ...

Embodiment 2

[0055] Example 2 Preparation of compound microbial agent

[0056] 1, the preparation of spore powder is with embodiment 1.

[0057] 2. Accurately weigh the spore powder of I. fumigata and Trichoderma harzianum spore powder prepared in Example 1, and mix them according to the weight ratio of 4:3 to obtain the composite spore powder.

[0058] 3. Add various additives to the low-temperature airflow mill, control the temperature of airflow milling below 10°C, and grind it for later use; it is preferred to grind it according to the fineness of 200 mesh.

[0059] 4. Add the mixture of various additives obtained by crushing in 3 into the carrier according to the proportion, stir evenly, then add the weighed composite spore powder in 2, and mix evenly to obtain the compounded agricultural compound microbial bacterial agent.

[0060] The agricultural composite microbial bacterial agent prepared by the present embodiment comprises the following components by weight percentage: 30% co...

Embodiment 3

[0063] Example 3 Preparation of I. fumigatus Bacteria Agent

[0064] 1. The preparation of the rose-colored Isosporium spore powder is the same as in Example 1.

[0065] 2. Accurately weigh the rose-colored Isospora spore powder prepared in Example 1.

[0066] 3. Add various additives to the low-temperature airflow mill, control the temperature of airflow milling below 10°C, and grind it for later use; it is preferred to grind it according to the fineness of 200 mesh.

[0067] 4. Add the mixture of various additives obtained by crushing in 3 into the carrier according to the proportion, stir evenly, then add the spore powder weighed in 2, and mix evenly to obtain the mixed agricultural fungus agent.

[0068]The agricultural I. fumigatus agent prepared by the present embodiment comprises the following components by weight percentage: 30% I. fumigatus spore powder, 3% NP-10, 2% sodium lauryl sulfate, 5 % sodium carboxymethylcellulose, 0.3% ascorbic acid, 59.7% diatomaceous e...

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Abstract

The invention provides a composite bacterial powder, a composite microbial agent thereof and a preparation method and application of the composite microbial agent. The composite bacterial powder is formed by compounding spore powder of trichoderma harzianum and spore powder of isaria fumosorosea. Isaria fumosorosea and trichoderma harzianum are combined for use, diseases and insect pests such as aphids, cabbage caterpillars, whiteflies, tomato leaf mold, pepper phytophthora blight and cucumber fusarium wilt can be effectively prevented and controlled, and the method has the advantages of beingstable in effect, safe, efficient and the like. The agricultural composite microbial agent can effectively reduce the use amount of pesticides, reduce pesticide residues and delay the pesticide resistance of pests, and has the potential of replacing part of chemical pesticides.

Description

technical field [0001] The invention relates to the technical field of pesticides, in particular to a composite bacterial powder, a composite microbial bacterial agent containing the bacterial powder, and a preparation method and application thereof. Background technique [0002] The information disclosed in this background section is only intended to increase the understanding of the general background of the present invention, and is not necessarily taken as an acknowledgment or any form of suggestion that the information constitutes the prior art already known to those skilled in the art. [0003] Due to human activities and changes in planting patterns, pests and diseases occur more and more frequently. Pests and diseases can reduce the yield and quality of crops, destroy trees, and bring huge economic losses. The inventors found that the current prevention and control of agricultural diseases and insect pests is still dominated by chemical agents, but the repeated and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14A01N63/38A01N63/30A01P7/04A01P3/00A01P1/00C12R1/885C12R1/645
CPCA01N63/30A01N63/38C12N1/14
Inventor 胡著然齐素敏韩广泉陈丹丹李圆圆冉新炎陶宁黄悦王丽荣张文娟申小冉徐曙光李敏
Owner SHANDONG BEE LAN BIOTECHNOLOGY CO LTD
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