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Application of Gab1 inhibitor to treatment of bile duct cancer

An inhibitor, the technology of cholangiocarcinoma, applied in the field of medicine, can solve the problem of low survival rate

Inactive Publication Date: 2020-08-14
淮安市厚沐医疗技术咨询中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Surgery is an effective treatment for cholangiocarcinoma, but the 5-year survival rate after surgery is not high

Method used

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  • Application of Gab1 inhibitor to treatment of bile duct cancer
  • Application of Gab1 inhibitor to treatment of bile duct cancer
  • Application of Gab1 inhibitor to treatment of bile duct cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: pharmacological activity assay

[0020] 1. Materials and methods

[0021] 1. Test material:

[0022] Human cholangiocarcinoma QBC939 cells were purchased from ATCC Cell Bank. Use DMEM medium containing 10% fetal bovine serum, 100 U / ml penicillin, 100 μg / ml streptomycin at 37°C, 5% CO 2 Cultivate in an incubator and pass passage for 48-72 hours.

[0023] The chemical structural formulas of test compounds 1-3 are as follows: figure 1 As shown, the purity is not less than 98%.

[0024] 2. MTT method to determine the IC50 value of the compound on the inhibition of the proliferation of cholangiocarcinoma cells

[0025] Take human cholangiocarcinoma QBC939 cells in the logarithmic growth phase, and use 4×10 cells per well. 4 Cells were cultured in a 96-well plate at a density of 96 wells, and the 96-well plate was tapped lightly to make the cells evenly distributed. After 24 hours of adaptive culture, they were replaced with different concentrations (0.625...

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Abstract

The invention discloses application of a Gab1 inhibitor to treatment of bile duct cancer. Experiments show that the test compounds 1 and 3 have a remarkable proliferation inhibition effect on human cholangiocarcinoma QBC939 cells, and the possible action mechanism is to inhibit the expression of Gab1 protein in the human cholangiocarcinoma QBC939 cells. Test compounds 1, 3 have the potential of being developed into drugs for treating the human bile duct cancer.

Description

technical field [0001] The invention belongs to the field of medicine, and in particular relates to the use of a Gab1 inhibitor for treating cholangiocarcinoma. Background technique [0002] Cholangiocarcinoma (CCA) is a highly malignant tumor originating from the epithelial cells lining the bile duct, and is the second most common primary liver malignancy, second only to hepatocellular carcinoma, accounting for 10% of all primary liver malignancies. %~20%. Cholangiocarcinoma is highly malignant and has a very poor prognosis. Surgery is an effective treatment for cholangiocarcinoma, but the 5-year survival rate after surgery is not high. The incidence of cholangiocarcinoma is increasing year by year worldwide. The average age of onset of cholangiocarcinoma is about 50 years old, and the incidence rate of men is about 1.5 times that of women. The diagnosis and treatment of cholangiocarcinoma has always been a hot spot in clinical research and drug research and development...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/433A61P35/00A61P1/16
CPCA61K31/433A61P35/00A61P1/16
Inventor 洪其昌陈东林
Owner 淮安市厚沐医疗技术咨询中心