A kind of anti-cancer pharmaceutical composition
A composition and drug technology, applied in the field of cancer drugs to achieve the effect of less toxic and side effects
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Embodiment 1
[0027] Example 1 Inhibiting the Survival Effect of Liver Cancer Cells
[0028] In order to detect the effect of phyllidine, lactoferrin, and phyllidine combined with lactoferrin on the survival of HepG2 cells, HepG2 cells were seeded in 96-well culture dishes (10 5 cells / well, 100 mL medium per well), after 24 hours of culture, 10 mg / L, 50 mg / L, 100 mg / L, 200 mg / L, 500 mg / L and 1 g / L Nantianine, 10 mg / L, 50mg / L, 100 mg / L, 200 mg / L, 500 mg / L and 1 g / L lactoferrin, and the combined group of Nantianine and lactoferrin ( 200 mg / L dose of nantiana combined with 100 mg / L dose of lactoferrin (mass ratio 2:1), 100 mg / L dose of nantiana combined with 100 mg / L dose of lactoferrin (mass ratio 1:1), 100 mg / L dose of nantiana combined with 200 mg / L dose of lactoferrin (mass ratio 1:2) (a total of 20 microliters for each group, 10 microliters for each drug in the combined administration group), for 24 hours , then discard the medium, add MTT (final concentration 5 g / L) to act for 4 hours,...
Embodiment 2
[0031] Example 2 Effect of Bambooline and Lactoferrin in Inhibiting HepG2 Tumor Growth in Nude Mice
[0032]The following groups were set up: no treatment control group, 25 mg / kg dose of nantiana, 50 mg / kg dose of lactoferrin, 25 mg / kg dose of nantiana combined with 50 mg / kg lactoferrin, 12.5 mg / kg dose Nantianning bamboo base combined with 25 mg / kg lactoferrin was used to detect the growth of HepG2 tumor in nude mice.
[0033] The experimental method is as follows: HepG2 cells were cultured on a large scale in 20 10cm culture dishes, and when the cell density reached about 80%, the cells were collected and injected subcutaneously into the right armpit of 20 BALb / C nude mice. After about three weeks, the tumor volume reached 90-110mm 3 . Mice (body weight 20 ± 1 g) were divided into 5 groups of 4 mice each. With 25mg / kg dose of nantiana, 50 mg / kg dose of lactoferrin, 25 mg / kg dose of nantiana combined with 50 mg / kg lactoferrin, 12.5 mg / kg dose of nantiana combined with 25mg...
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