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Application of nucleoside hydrogel in preparation of medicine for preventing or delaying canceration of oral mucosa potential malignant diseases

A technology of oral mucosa and nucleoside water, applied in the field of medicine, can solve problems such as poor effect, and achieve the effect of inhibiting the proliferation of diseased cells and delaying the canceration of oral leukoplakia

Pending Publication Date: 2020-08-21
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The study found that 90% of early-stage patients can be cured with a single surgical treatment, but among the diagnosed patients, the early-stage cases are less than 10%; in addition, more than 70% of OSCC patients undergoing radical surgical treatment are often accompanied by appearance deformity and functional disorders and other complications
However, the currently available topical drugs are not effective in preventing and treating OLK carcinogenesis.

Method used

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  • Application of nucleoside hydrogel in preparation of medicine for preventing or delaying canceration of oral mucosa potential malignant diseases
  • Application of nucleoside hydrogel in preparation of medicine for preventing or delaying canceration of oral mucosa potential malignant diseases
  • Application of nucleoside hydrogel in preparation of medicine for preventing or delaying canceration of oral mucosa potential malignant diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1: Preparation of nucleoside hydrogel

[0027] The isoGBG hydrogel was prepared by the same method as in Example 1 of the Chinese patent application with the application number 201910340339.9 and the title of the invention "a bifunctional nucleoside hydrogel and its preparation method and application".

[0028] Specific steps are as follows:

[0029] Isoguanosine (isoG) and NaB(OH) at a molar ratio of 1:1 4 Dissolve in ultrapure water, heat until fully dissolved to obtain a clear and transparent solution, and the concentration of isoguanosine in the obtained solution is 1.4wt%. In the guanosine (G) added in the solution, the molar ratio of guanosine to isoguanosine is 1:1, that is, the concentration of guanosine is also 1.4wt%. After the lower solution is naturally cooled, a transparent and colorless hydrogel is obtained, namely the nucleoside hydrogel of the present invention, which is named as isoGBG hydrogel. The concentration of the isoGBG hydrogel was...

experiment example 1

[0032] Experimental example 1: The effect of isoGBG hydrogel on the proliferation of DOK cells

[0033] 1. Experimental method

[0034] (1) Test cell viability

[0035] Well-growing DOK cells (that is, precancerous lesion cells of human oral mucosa) were prepared into a cell suspension, and the density was adjusted and seeded in a 96-well plate. After the cells adhered to the wall, 10 μl of PBS and different concentrations of isoGBG hydrogel (with The isoGBG hydrogel prepared in Example 1 was diluted with PBS, and the final concentrations were 50 μg ml -1 , 75μg·ml -1 , 100μg·ml -1 , 200μg·ml -1 , 400μg·ml -1 , 800μg·ml -1 ), after culturing for 24h and 48h respectively, add 10% CCK8 reagent, and incubate in a 37°C incubator for about 1h. After the color change in the well plate is obvious, use a microplate reader to detect the absorbance value (OD ), three auxiliary wells were set for each group, and the average value was taken to calculate the cell survival rate, and ...

experiment example 2

[0045] Experimental Example 2: In vivo experiment of isoGBG hydrogel delaying canceration of OLK

[0046] 1. Experimental method

[0047] Dissolve 5mg of 4-NQO (4-nitroquinoline-oxide, 4-nitroquinoline-1-oxide) in 45ml of propylene glycol, prepare a 5mg / mL mother solution, and store it in a 4°C refrigerator after ultrasonic dissolution. Take 4ml of the above mother solution and add it to 200ml of physiological saline to obtain a concentration of 100μg·ml -1 4-NQO solution, the solution was fed to mice to construct oral leukoplakia (OLK) model.

[0048] The mice were observed for abnormalities in hair, body weight, and behavior, and the development of mucosal lesions on the tongue, cheeks, and floor of the mouth of the mice were checked after isoflurane inhalation anesthesia every week. After irregular white lesions appeared on the tongues of all the mice, one of the mice was randomly selected. After the tongue dissection showed that the modeling was successful, 24 mice were ...

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Abstract

The invention discloses an application of nucleoside hydrogel in preparation of a medicine for preventing or delaying canceration of oral mucosa potential malignant diseases. The nucleoside hydrogel is prepared by dissolving isoguanine nucleoside, guanine nucleoside and borate in water or an aqueous solution and then crosslinking. The experiments prove that the nucleoside hydrogel provided by theinvention can effectively inhibit proliferation of human oral mucosa precancerous lesion cells and delay canceration of oral leukoderma. Therefore, the nucleoside hydrogel provided by the invention has a good application prospect in preparation of drugs for preventing or delaying oral mucosa potential malignant diseases, especially oral leukoplakia canceration.

Description

technical field [0001] The invention belongs to the field of medicine, and specifically relates to the use of nucleoside hydrogel in the preparation of drugs for preventing or delaying potential malignant diseases of oral mucosa, especially oral leukoplakia. Background technique [0002] Oral cancer is the sixth most malignant tumor in the whole body, among which oral squamous cell carcinoma (Oral squamous cell carcinoma, OSCC) has the highest incidence rate, accounting for about 90% of the primary malignant tumors of oral mucosa. OSCC is usually discovered and diagnosed late, and has the characteristics of early metastasis, high postoperative recurrence rate, and poor prognosis, resulting in a high mortality rate of OSCC. As of 2018, 177,384 patients worldwide still died of OSCC. The study found that 90% of early-stage patients can be cured with a single surgical treatment, but among the diagnosed patients, the early-stage cases are less than 10%; in addition, more than 70%...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/7076A61K33/22A61K9/06A61P35/00A61P1/02
CPCA61K31/7076A61K33/22A61K9/06A61K9/006A61P35/00A61P1/02A61K2300/00
Inventor 赵行唐帆刘江但红霞陈谦明
Owner SICHUAN UNIV
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