Scutellaria barbata polysaccharide, extraction method and application in preparation of anti-sars-cov-2 medicine

A sars-cov-2, Scutellaria barbata technology, applied in the field of medicine

Active Publication Date: 2022-03-22
爱礼康生物医药(苏州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there is currently no report on the anti-SARS-CoV-2 of Scutellaria barbatae

Method used

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  • Scutellaria barbata polysaccharide, extraction method and application in preparation of anti-sars-cov-2 medicine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: the extracting method of Scutellaria barbata polysaccharide comprises the following steps:

[0025] Step 1, crushing the dried Scutellaria barbata plant to obtain Scutellaria barbata powder;

[0026] Step 2. Add 14 times the mass of deionized water to Scutellaria barbata powder, adjust its pH value to 4.8 to obtain a mixed solution, heat the mixed solution to 55°C, and then add cellulase, pectinase and papain enzymes respectively Decompose 40min, obtain enzymolysis solution; Wherein, the addition amount of cellulase, pectinase and papain is respectively 0.6%, 0.6%, 0.5% of Scutellaria barbata powder mass;

[0027] Step 3: Heating the enzymolysis solution to 105°C and maintaining it for 1 hour, then cooling and filtering to obtain the filtrate;

[0028] Step 4, heating and concentrating the filtrate to obtain a concentrate, and then dialyzing the concentrate with flowing water for 2 days to obtain a dialysate;

[0029] Step 5, heat and concentrate the dia...

Embodiment 2

[0031] Embodiment 2: the extracting method of Scutellaria barbata polysaccharide comprises the following steps:

[0032] Step 1, crushing the dried Scutellaria barbata plant to obtain Scutellaria barbata powder;

[0033] Step 2. Add 12 times the mass of deionized water to Scutellaria barbata powder, adjust its pH value to 4.6 to obtain a mixed solution, heat the mixed solution to 60°C, and then add cellulase, pectinase and papain enzymes respectively Decompose 45min, obtain enzymatic hydrolysis solution; Wherein, the addition amount of cellulase, pectinase and papain is respectively 0.5%, 1%, 0.7% of Scutellaria barbata powder mass;

[0034] Step 3, heating the enzymolysis solution to 110°C and keeping it for 1 hour, then cooling and filtering to obtain the filtrate;

[0035] Step 4, heating and concentrating the filtrate to obtain a concentrate, and then dialyzing the concentrate with running water for 3 days to obtain a dialysate;

[0036] Step 5, heat and concentrate the ...

Embodiment 3

[0038] Embodiment 3: the extracting method of Scutellaria barbata polysaccharide, comprises the following steps:

[0039] Step 1, crushing the dried Scutellaria barbata plant to obtain Scutellaria barbata powder;

[0040] Step 2. Add 15 times the mass of deionized water to Scutellaria barbata powder, adjust its pH value to 4.7 to obtain a mixed solution, heat the mixed solution to 50°C, and then add cellulase, pectinase and papain enzymes respectively Decompose 35min, obtain enzymolysis solution; Wherein, the addition amount of cellulase, pectinase and papain is respectively 0.8%, 0.5%, 0.4% of Scutellaria barbata powder mass;

[0041] Step 3, heating the enzymolysis solution to 100°C and keeping it for 1.2h, then cooling and filtering to obtain the filtrate;

[0042] Step 4, heating and concentrating the filtrate to obtain a concentrate, and then dialyzing the concentrate with running water for 3 days to obtain a dialysate;

[0043] Step 5, heat and concentrate the dialysat...

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Abstract

This scheme discloses barbata polysaccharides in the field of medical technology, its extraction method and its application in the preparation of anti-SARS‑CoV‑2 drugs. The preparation of barbata polysaccharides comprises the following steps: 1. crushing dried barbatae plants to obtain barbatae powder; 2. adding deionized water to barbatae powder and adjusting its pH value to 4.6-4.8 to obtain Mixed solution, heat the mixed solution to 50-60°C, then add cellulase, pectinase and papain to enzymatically hydrolyze for 35-45 minutes to obtain an enzymatic hydrolysis solution; 3. Heat the enzymatic hydrolysis solution to 100-110°C and keep 1 to 1.2 hours, then cool and filter to obtain the filtrate; 4. Heat and concentrate the filtrate to obtain a concentrate, and then dialyze the concentrate through flowing water for 2 to 3 days to obtain a dialysate; 5. Heat and concentrate the dialysate, centrifuge, remove After precipitation, add 95% ethanol of 5 to 6 times the volume of the supernatant, and let it stand overnight for alcohol precipitation to obtain an alcohol precipitation solution; 6. Centrifuge the alcohol precipitation solution to obtain a precipitate, and alternately wash the precipitation with absolute ethanol and acetone for 3- Scutellaria barbata was obtained 5 times.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to barbata polysaccharides, an extraction method and an application in preparing anti-SARS-CoV-2 medicines. Background technique [0002] 2019 Novel Coronavirus, also known as SARS-CoV-2. Common signs of human infection with SARS-CoV-2 include respiratory symptoms, fever, cough, shortness of breath, and dyspnea. In more severe cases, infection can lead to pneumonia, severe acute respiratory syndrome, kidney failure, and even death. Currently, there is no specific treatment for the disease caused by the novel coronavirus. [0003] The new coronavirus pneumonia virus SARS-Cov-2 is a typical coronavirus 2-5, and the diameter of the virus particle is about 60-220nm. There are three kinds of glycoproteins on the membrane surface: spike glycoprotein (S, Spike Protein), small envelope glycoprotein (E, Envelope Protein) and membrane glycoprotein (M, Membrane Protein). Among...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/00A61K31/715A61P31/14A61P11/00
CPCC08B37/0003C08B37/006A61K31/715A61P31/14A61P11/00
Inventor 丁侃陈霞孙成新姚晓东王秋雨张艳丽
Owner 爱礼康生物医药(苏州)有限公司
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