Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Research method of MS-275 acting on acute myocardial ischemia reperfusion injury

A technology of acute myocardial ischemia and MS-275, which is applied in the medical field and can solve problems such as less research on molecular mechanisms

Pending Publication Date: 2020-08-25
THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the specific molecular mechanisms involved in this protective effect have been less studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0016] The research method of MS-275 acting on acute myocardial ischemia-reperfusion injury comprises the following steps:

[0017] Step 1: Establish a rat acute MI / RI model by ligation of the left anterior descending coronary artery in SD male rats: 72 SD male rats were randomly divided into 4 groups, 18 in each group: the first group was Sham operation group (Sham group), only threading without ligation; the second group is ischemia 30 minutes group (I 30min group); the third group is ischemia 30 minutes reperfusion 60 minutes group (I / R60min group); The group was ischemia for 30 minutes and reperfusion for 2 hours (I / R 120min group); after the experiment, the rats were executed, the heart was separated, and the tissue samples of the myocardial infarction area were taken, and the section was stained to observe the range of myocardial infarction and the cell tissue of the myocardial infarction area. Morphological changes, and histone acetylation level, HDAC1 and miR-210 expre...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a research method of MS-275 acting on acute myocardial ischemia reperfusion injury. The research method comprises the following steps: step I, performing ligation of an anterior descending branch of a left coronary artery of an SD male bandicoot to construct a bandicoot acute MI / RI model; step II, observing the action of the MS-275 in an MI / RI acute stage of the bandicoot;and step III, discussing a molecular mechanism of the MS-275 on bandicoot MI / RI protection action. According to the research, through constructing the bandicoot acute MI / RI model and combination within vivo and vitro experiments, the following research objectives are achieved: (1) observing the acetylation level of histone, and expression of HDAC1 and microRNA-210(miR-210) in cardiac muscle tissue of the bandicoot at the MI / RI acute stage; (2) confirming that the MS-275, a novel HDACI type selective depressant, has protection action on MI / RI of the bandicoot and influence on the acetylation level of the histone and expression of HDAC1 and miR-210 in tissue of a myocardial infarction region of the bandicoot subjected to myocardial ischemia reperfusion; and (3) disclosing a specific molecular mechanism that the MS-275 improves MI / RI of the bandicoot from the angle of miRNA epigenetic regulation, and providing an important theory and experiment basis for MI / RI treatment with HDACi.

Description

technical field [0001] The invention relates to the field of medical technology, in particular to a research method of MS-275 acting on acute myocardial ischemia-reperfusion injury. Background technique [0002] A large number of studies have shown that histone deacetylase, as an important regulator of epigenetic modification, is involved in the pathogenesis of myocardial ischemia / reperfusion injury. Histone deacetylase inhibitors can interfere with the function of HDACs, effectively reduce ischemia / reperfusion injury, and have broad therapeutic prospects in the field of myocardial protection. However, the specific molecular mechanisms involved in this protective effect are less studied. Contents of the invention [0003] In order to overcome the above problems, the present invention provides a research method for MS-275 acting on acute myocardial ischemia-reperfusion injury. [0004] The technical scheme adopted in the present invention is: [0005] The research method...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12Q1/6883C12Q1/686G01N33/68A61K49/00
CPCC12Q1/6883C12Q1/686G01N33/68A61K49/0008C12Q2600/178G01N2800/2871C12Q2561/113
Inventor 郭斌李朵徐军美
Owner THE SECOND XIANGYA HOSPITAL OF CENT SOUTH UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com