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Application of milk-derived active peptide CCL-3S in preparation of sleep promoting products

A sleep-promoting and active peptide technology, applied in the application field of milk-derived active peptide CCL-3S in the preparation of sleep-promoting products, can solve the problems of less research

Active Publication Date: 2020-09-08
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There are many studies on the antibacterial, anti-oxidant, antihypertensive, and immune regulation effects of milk-derived peptides, but less research on the regulation of the nervous system by milk-derived peptides

Method used

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  • Application of milk-derived active peptide CCL-3S in preparation of sleep promoting products
  • Application of milk-derived active peptide CCL-3S in preparation of sleep promoting products
  • Application of milk-derived active peptide CCL-3S in preparation of sleep promoting products

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Screening and identification of milk-derived active peptides of embodiment 1

[0030] According to the patented method of application number 201510915215.0, titled "Method for Screening Crude Sleep-promoting Peptides in Milk Sources Using Patch Clamp Technology", casein hydrolyzate was separated from milk, and then a milk-derived active peptide was screened from it. Named CCL-3S, it was identified that the milk-derived active peptide has an amino acid sequence of HQGLPQEVLNENLLR and a molecular weight of 1759.998Da. Among them, the high performance liquid chromatogram of casein hydrolyzate is shown as figure 1 As shown, the high-performance liquid chromatogram and mass spectrogram of the milk-derived active peptide CCL-3S are respectively shown in figure 2 and image 3 shown.

Embodiment 2

[0031] Electrophysiological-sleep effect of embodiment 2 milk-derived active peptides

[0032] Acutely separate the brain slices of the ventral lateral optic nucleus of the rat hypothalamus, with a thickness of about 300-500um, using the electrophysiological patch clamp technique, according to the effect of the milk-derived active peptide of Example 1 on the neurons of the ventral lateral preoptic area of ​​the rat hypothalamus The discharge characteristics of the milk-derived active peptide were used to judge the sleep-promoting activity of the milk-derived active peptide. For the specific test method, refer to the patent with the application number 201510915215.0 and the title "Method for screening crude extracts of sleep-promoting peptides in milk sources using patch clamp technology". Its electrophysiological-sleep effects such as Figure 4 shown.

[0033] according to Figure 4 The results show that after the milk-derived active peptide acts on the cells, the change ra...

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Abstract

The invention belongs to the field of sleep-promoting health-care products, and particularly relates to application of milk-derived active peptide CCL-3S in preparation of sleep promoting products. Aiming at developing more sleep promoting peptides with a nervous system regulating effect and application thereof, the milk-derived active peptide CCL-3S capable of inhibiting nerve cell action potential release frequency and promoting sleep is separated and identified from milk by using an in-vitro patch clamp technology. The milk-derived active peptide has an amino acid sequence as shown in SEQ ID NO.1 and a molecular weight of 1759.998 Da, can be applied to preparation of sleep-promoting medicines, sleep-promoting functional foods and other products, can fully utilize milk product resources,and provides a new way for high-valued utilization of milk-derived polypeptides. Meanwhile, the sleep-promoting milk-derived active peptide provided by the invention is safe and non-toxic.

Description

technical field [0001] The invention belongs to the field of sleep-promoting health care products, and in particular relates to the application of milk-derived active peptide CCL-3S in the preparation of sleep-promoting products. Background technique [0002] Sleep accounts for about one-third of a person's life, and good sleep is the main sign of physical and mental health. However, with the development of modern society, people's pace of life is generally accelerated, and there are more and more insomnia caused by various pressures. Insufficient sleep directly leads to slow thinking, nervousness, inattention, slow action, boredom, anxiety, burnout, lack of enthusiasm for work, reduced work initiative and willingness to work, thereby reducing work efficiency. In the long run, people who lack sleep will feel particularly tired, emotionally unstable, irritable, irritable, and lack self-control, causing serious psychological problems. In addition, insomnia can also lead to d...

Claims

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Application Information

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IPC IPC(8): A61K38/10A61P25/20A23L33/18
CPCA61K38/10A61P25/20A23L33/18A23V2002/00A23V2200/322
Inventor 曹庸梁曹雯陈媛媛苗建银李俊刘果
Owner SOUTH CHINA AGRI UNIV
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