Novel sleep-promoting milk-derived active peptide CCL-2S as well as preparation method and application thereof

A CCL-2S, sleep-promoting technology, applied in the preparation methods, applications, chemical instruments and methods of peptides, etc., can solve the problems of inability to meet sub-healthy people, low content of active polypeptides, etc., and achieve excellent sleep-promoting activity and inhibiting release. The effect of frequency

Pending Publication Date: 2021-12-07
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Milk has a good sleep-promoting effect. Milk contains a variety of biologically active peptides, but the content of active peptides in natural milk is small, which cannot meet the needs of sub-healthy people

Method used

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  • Novel sleep-promoting milk-derived active peptide CCL-2S as well as preparation method and application thereof
  • Novel sleep-promoting milk-derived active peptide CCL-2S as well as preparation method and application thereof
  • Novel sleep-promoting milk-derived active peptide CCL-2S as well as preparation method and application thereof

Examples

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Embodiment Construction

[0035] In order to better understand the present invention, the technical solutions of the present invention will be described below through specific examples. The medicines and reagents used in the following examples are all commercially available, and the milk-derived casein hydrolyzate used is a milk polypeptide solution obtained by enzymatic hydrolysis of milk casein with protease. The specific preparation method is as follows: mix casein with distilled water at a ratio of 1:10 (w / v), then add 0.5% trypsin to the casein solution for hydrolysis for 5 hours, adjust the pH value to 4-5, centrifuge to get the supernatant, and then extinguish the enzymatic hydrolysis product (supernatant) at high temperature Afterwards, the supernatant was collected by centrifugation, freeze-dried, and stored at -20°C for later use.

[0036] The novel sleep-promoting milk-derived active peptide of the present invention is screened and prepared by the following method:

[0037] (1) Raw materia...

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Abstract

The invention relates to a novel sleep-promoting milk-derived active peptide CCL-2S. The amino acid sequence of the CCL-2S is FALPQYLK. According to the invention, milk-derived casein hydrolysate is taken as a raw material. After basic means such as desalination are adopted to remove impurities, an in-vitro patch clamp technology is utilized to separate and identify the novel sleep-promoting milk-derived active peptide CCL-2S which can inhibit the action potential release frequency of nerve cells and promote sleep from milk. In addition, the novel sleep-promoting milk-derived active peptide is safe and non-toxic, can be applied to preparation of products such as sleep-promoting functional foods and the like, can fully utilize milk product resources, and provides a new approach for high-value utilization of the milk-derived peptide.

Description

technical field [0001] The invention belongs to the technical field of extracting active ingredients from milk, and in particular relates to a novel sleep-promoting milk-derived active peptide CCL-2S and its preparation method and application. Background technique [0002] Sleep accounts for about one-third of a person's life, and good sleep is the prerequisite for physical and mental health. With the development of modern society, people's pace of life is generally accelerated, and there are more and more insomnia caused by various pressures. Insufficient sleep will directly lead to slow thinking, nervousness, inattention, slow action, boredom, worry, burnout, lack of enthusiasm for work, reduced work initiative and willingness to work, thereby reducing work efficiency. In the long run, people who lack sleep will feel particularly tired, emotionally unstable, irritable, irritable, and lack self-control, causing serious psychological problems. In addition, insomnia can als...

Claims

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Application Information

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IPC IPC(8): C07K14/47C07K1/20C07K1/34C07K1/36A61K38/17A61P25/20A23L33/18
CPCC07K14/4732A61P25/20A23L33/18A61K38/00A23V2002/00A23V2200/322A23V2250/55Y02A50/30
Inventor 曹庸陈媛媛梁曹雯刘星雨刘飞
Owner SOUTH CHINA AGRI UNIV
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