Clove extract and application of compound of clove extract to preparation of composition for regulating gene expression quantity

A technology for extracts and pharmaceutical compositions is applied in the field of clove extract and its compounds for preparing compositions for regulating gene expression, which can solve the problems of complex chronic inflammation mechanism and the like

Inactive Publication Date: 2020-09-15
TCI CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, since the mechanism of chronic inflammation is much more complicated than that of acute inflammation, it cannot be easily improved by the above-mentioned single drug. Therefore, at present, patients can only be advised to improve the symptoms of chronic inflammation by improving eating habits, daily life, etc.

Method used

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  • Clove extract and application of compound of clove extract to preparation of composition for regulating gene expression quantity
  • Clove extract and application of compound of clove extract to preparation of composition for regulating gene expression quantity
  • Clove extract and application of compound of clove extract to preparation of composition for regulating gene expression quantity

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1 The preparation method of clove extract of the present invention

[0059] In one embodiment of the present invention, the extraction solvent of lilac flower buds and water, alcohol, or alcohol-water mixture is mixed with a liquid-solid ratio of 1-5:5-25, and the extraction solvent is preferably 30-70% ethanol. After quality, extract in a solvent at 0-50°C for 0.5-3 hours, and then filter through a 400 mesh filter to obtain a filtrate. Finally, the filtrate is concentrated under reduced pressure at 30-60° C. to obtain the clove extract of the present invention.

Embodiment 2

[0060] Example 2 Separation of effective substances with anti-inflammatory activity in the clove extract of the present invention

[0061] In the embodiment of the present invention, in order to separate the active components in the clove extract of the present invention, the clove extract was first subjected to column chromatography with gradient concentrations of methanol, and the efficacy of each layer of extracts in regulating the expression of cell media-related genes was tested. First, take 2L of the filtrate described in Example 1 (i.e. the clove extract stock solution), and concentrate it under reduced pressure at 30-60°C until the total volume is 500mL. The concentrated solution is the clove extract of the present invention. Resin (Diaion HP-20, 100cm x 8cm) was subjected to column chromatography, the gradient was from water to methanol, and a total of 5 layers were obtained, namely layer 1 was 0% methanol, layer 2 was 20% methanol, layer 3 40% methanol, 60% methanol ...

Embodiment 3

[0076] Example 3 The active ingredients TCI-SA-01, TCI-SA-02, and TCI-SA-03 of the clove extract of the present invention regulate IL-1β gene, IL-18 gene, IFNG gene, TGF-β gene, Effects of IL-8 gene, IL-16 gene, and IL-23 gene expression

[0077] The embodiment of the present invention is to test that the compounds TCI-SA-01, TCI-SA-02, and TCI-SA-03 isolated from the clove extract of the present invention have the ability to reduce the expression of pro-inflammatory-related cell mediator genes and enhance The effect of anti-inflammation-related cell mediator gene expression, the THP-1 cells were used to test the regulation of cell mediator-related gene expression by the three compounds, in which the endotoxin of Gram-positive bacteria was used as an antigen to induce THP-1 The cells mount an immune response. First, place the 1.5x10 5 THP-1 cells were cultured in each well of a 6-well culture plate, cultured at 37°C for 16-18 hours, and then the cells were divided into the f...

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Abstract

The invention relates to the field of plant extracts, in particular to a clove extract and application of a compound of the clove extract to preparation of a composition for regulating gene expressionquantity. The invention provides a clove extract and application of a compound of the clove extract to regulation and control of expression quantity of IL-1 beta genes, IL-18 genes, IFNG genes, TGF-beta genes, IL-8 genes, IL-16 genes and / or IL-23 genes. The expression quantity of related genes capable of promoting inflammatory response can be effectively reduced, and meanwhile, the expression quantity of related genes capable of inhibiting inflammatory response can be effectively improved. The clove extract is obtained by extracting clove with water, alcohol or a solvent of an alcohol-water mixture, and the clove extract contains effective components of isorhamnetin-3-methyl gluconate, kaempferol-3-methyl gluconate and / or 3, 3', 4-trimethyl ellagic acid.

Description

technical field [0001] The invention relates to the field of plant extracts, in particular to the use of clove extracts and their compounds for preparing compositions for regulating gene expression. Background technique [0002] Inflammatory response is the defensive response of tissues in the vascular system to inflammatory factors and local damage. It is mainly a physiological response stimulated by trauma, bleeding, or pathogenic infection. It includes symptoms such as redness, fever, and pain. Inflammation is a protective measure of the innate immune system to remove harmful stimuli or pathogens and promote tissue repair. Usually, inflammation is beneficial. However, sometimes inflammation may cause an allergic reaction of the body's own immune system, which can lead to Allergic conditions of the immune system such as rheumatoid arthritis and lupus. Among them, steroids, non-steroidal anti-inflammatory drugs, and antihistamines are commonly used in medicine to suppress ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/61A61K31/7048A61P29/00A61K8/9789A61K8/60A61K8/49A61Q19/00A23L33/105A61K31/366
CPCA61K36/61A61K31/7048A61K31/366A61P29/00A61K8/9789A61K8/602A61K8/498A61Q19/00A23L33/105A23V2002/00
Inventor 林咏翔
Owner TCI CO LTD
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