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Novel coronavirus 2019-nCoV antibody spectrum detection kit

A 2019-ncov, detection kit technology, applied in the field of novel coronavirus 2019-nCoV antibody spectrum detection kits, can solve problems such as high instrument and operation requirements, cross-reaction, false positives, etc. The effect of less production cost reduction

Pending Publication Date: 2020-09-15
无锡市孚维尔生物医疗科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The disadvantage of this method is that the lateral flow chromatography method uses one type of at most two types of proteins for detection, and there is no cleaning process. Since the blood of individual patients contains rheumatoid factors, heterophilic antibodies, autoantibodies, and drugs and tumor cells, it is easy to detect. Causes cross-reaction of tests, resulting in false positive results
There are also a small number of western blotting methods, but the preparation of test strips requires electrophoresis and membrane transfer operations, and the requirements for instruments and operations are high.

Method used

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  • Novel coronavirus 2019-nCoV antibody spectrum detection kit

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0081] Preparation of test strips:

[0082] 1. Reagent preparation:

[0083] 1.1 Protein dilution: Weigh 55.8g of disodium hydrogen phosphate, 5.5g of sodium dihydrogen phosphate, and 9g of sodium chloride; add 999mL of pure water to dissolve, draw 1mL of proClin300 into the solution, stir and mix, and dilute to 1000mL. Store at room temperature for later use.

[0084] 1.2 Preparation of blocking solution: Weigh 55.8 g of disodium hydrogen phosphate, 5.5 g of sodium dihydrogen phosphate, 9 g of sodium chloride, and 10 g of bovine serum albumin, add 900 mL of pure water to dissolve, add NaOH solution to adjust the pH to 7.4. Draw 1mL proClin300 into the solution, stir and mix, and dilute to 1000mL. Store at 2-8°C for later use.

[0085] 2. Preparation steps:

[0086] 2.1 The length and width of the nitrocellulose membrane were cut to 31cm×5cm, and four kinds of high-purity specific recombinant S1 protein, recombinant RBD protein, recombinant N protein, and recombinant E pro...

Embodiment 2

[0102] Preparation of test strips:

[0103] 1. Reagent preparation:

[0104] 1.1 Protein dilution: Weigh 55.8g of disodium hydrogen phosphate, 5.5g of sodium dihydrogen phosphate, and 9g of sodium chloride; add 999mL of pure water to dissolve, draw 1mL of proClin300 into the solution, stir and mix, and dilute to 1000mL. Store at room temperature for later use.

[0105] 1.2 Preparation of blocking solution: Weigh 55.8 g of disodium hydrogen phosphate, 5.5 g of sodium dihydrogen phosphate, and 9 g of sodium chloride; weigh 10 g of bovine serum albumin, add 900 mL of pure water to dissolve, add NaOH solution to adjust the pH to 7.4. Draw 1mL proClin300 into the solution, stir and mix, and dilute to 1000mL. Store at 2-8°C for later use.

[0106] 2. Preparation steps:

[0107] 2.1 Cut the nitrocellulose membrane to a size of 31cm×5cm in length and width, and draw three kinds of high-purity specific recombinant S1 protein, recombinant RBD protein, and recombinant N protein on th...

Embodiment 3

[0123] Preparation of test strips:

[0124] 1. Reagent preparation:

[0125] 1.1 Protein dilution: Weigh 55.8g of disodium hydrogen phosphate, 5.5g of sodium dihydrogen phosphate, and 9g of sodium chloride; add 999mL of pure water to dissolve, draw 1mL of proClin300 into the solution, stir and mix, and dilute to 1000mL. Store at room temperature for later use.

[0126] 1.2 Preparation of blocking solution: Weigh 55.8 g of disodium hydrogen phosphate, 5.5 g of sodium dihydrogen phosphate, and 9 g of sodium chloride; weigh 10 g of bovine serum albumin, add 900 mL of pure water to dissolve, add NaOH solution to adjust the pH to 7.4. Draw 1mL proClin300 into the solution, stir and mix, and dilute to 1000mL. Store at 2-8°C for later use.

[0127] 2. Preparation steps:

[0128] 2.1 The length and width of the nitrocellulose membrane are cut to 31cm×5cm, and three kinds of high-purity specific recombinant S1 protein, recombinant RBD protein, and recombinant E protein are respecti...

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Abstract

The invention relates to a novel coronavirus 2019-nCoV antibody spectrum detection kit, and belongs to the technical field of novel coronavirus 2019-nCoV detection. The detection kit provided by the invention comprises a barreled test strip, a bottled sample diluent, a bottled eluent, a bottled detection antibody, a bottled substrate A, a bottled substrate B, an incubation tank, tweezers, an instruction book and a self-sealing bag. According to the detection kit provided by the invention, the antibodies (IgM / IgG / IgA / IgE) in a serum or plasma sample are jointly detected by utilizing various novel coronavirus specific recombinant antigen test strips; the kit can be used for in-vitro qualitative detection of novel coronavirus antibodies in human serum or plasma samples, effectively increasessensitivity and specificity, timely finds early infected people, can be used for disease course monitoring of evaluation and diagnosis of antibodies in rehabilitation patients, and can be widely usedfor evaluation of vaccine human body effects in the future.

Description

technical field [0001] The invention relates to a novel coronavirus 2019-nCoV antibody spectrum detection kit, which belongs to the technical field of novel coronavirus 2019-nCoV detection. Background technique [0002] Western blotting is a hybridization technique that combines high-resolution gel electrophoresis and immunochemical analysis techniques. Western blotting has the advantages of large analysis capacity, high sensitivity, and strong specificity. It is the most commonly used method for detecting protein characteristics, expression, and distribution. It is used for qualitative and quantitative detection of tissue antigens, mass determination of polypeptide molecules, and virus detection. Antibody or antigen detection, etc. [0003] Traditional western blotting is performed in three stages. The first stage is SDS-polyacrylamide gel electrophoresis (SDS-PAGE): protein samples such as antigens are negatively charged after SDS treatment, and migrate from the cathode ...

Claims

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Application Information

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IPC IPC(8): G01N33/68G01N33/58G01N33/569G01N33/558
CPCG01N33/6854G01N33/581G01N33/56983G01N33/558G01N2333/165G01N2469/20
Inventor 吴玉章吴长龙
Owner 无锡市孚维尔生物医疗科技有限公司
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