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A kind of mutagenesis method of dianthus flower sprouts and its application

A technology of Dianthus and young shoots, applied in the biological field, can solve the problems of certain risks to the health of breeding operators, environmental pollution, improper protection, etc., and achieve the effects of overcoming obstacles to variety improvement, not polluting the environment, and simple operation.

Active Publication Date: 2021-08-06
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical mutagenesis often uses various rays, and improper protection will pose certain risks to the health of breeding operators; chemical mutagenesis uses various chemical reagents, which has certain pollution to the environment

Method used

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  • A kind of mutagenesis method of dianthus flower sprouts and its application
  • A kind of mutagenesis method of dianthus flower sprouts and its application
  • A kind of mutagenesis method of dianthus flower sprouts and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 10 μL of GV3101 Agrobacterium in an ultra-low temperature refrigerator and put it into 20ml YEB liquid medium, add rifampicin with a final concentration of 60mg / L, shake and culture at 28°C and 200 rpm for 20h, and obtain activated Agrobacterium liquid, Agrobacterium liquid OD 600 The value is 0.3.

[0036] Take 50 shoots of 4 cm in carnation variety 'Languiren', peel off the young leaves to expose the stem tip, prick a wound around the shoot tip with a dissecting needle, then soak the shoots in the activated GV3101 Agrobacterium solution, Shake culture at a temperature of 26°C and a speed of 30rpm in the dark for 16 hours; take out the shoots and wash them with water for 3 times, then immerse them in 400 mg / L cefotaxime sodium solution for 4 hours; take out the shoots and wash them with water for 3 times, and add rooting powder Insert into the substrate, the substrate is made of humus, perlite and coconut shell in a mass ratio of 1:1:1; take root after 20 days, p...

Embodiment 2

[0038] Take 20 μL of EHA105 Agrobacterium in an ultra-low temperature refrigerator and put it into 30ml of YEB liquid medium, add rifampicin with a final concentration of 70mg / L, shake and culture at 28°C and 200rpm for 22h, and obtain activated Agrobacterium liquid, Agrobacterium liquid OD 600 The value is 0.32.

[0039]Take 50 shoots of 3 cm in carnation variety 'Fenlian', peel off the young leaves to expose the stem tip, prick the wound around the shoot tip with a dissecting needle, then dip the shoots into the activated EHA105 Agrobacterium bacterium liquid, Shake culture at 27°C and 40 rpm in the dark for 24 hours; take out the shoots and rinse them with water for 3 times, then immerse them in 450 mg / L cefotaxime sodium solution for 6 hours; The rooting powder is inserted into the substrate, which is made of humus, perlite and coconut shell in a mass ratio of 2:2:1. Put the rooting seedlings into the greenhouse for cultivation, water and fertilize, and enter the flowering...

Embodiment 3

[0041] Take 30 μL of LBA4404 Agrobacterium in an ultra-low temperature refrigerator and put it into 40ml YEB liquid medium, add rifampicin with a final concentration of 65mg / L, shake and culture at 28°C and 200rpm for 24 hours, and obtain activated Agrobacterium liquid, Agrobacterium liquid OD 600 The value is 0.4.

[0042] Take 50 shoots of 3 cm in carnation variety 'Master', peel off the young leaves to expose the stem tip, prick the wound around the shoot tip with a dissecting needle, and then soak the shoots in the activated LBA4404 Agrobacterium solution , at a temperature of 27°C and a speed of 40 rpm in the dark for 24 hours; take out the shoots and rinse them with water for 3 times, and then immerse them in 500 mg / L cefotaxime sodium solution for 8 hours; The rooting powder is inserted into the substrate, which is made of humus, perlite and coconut shell in a mass ratio of 1:3:1; after 30 days of rooting, the rooting seedlings are placed in a greenhouse for cultivatio...

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Abstract

The invention belongs to the field of biotechnology, and in particular relates to a method for inducing bud change of flowers of the genus Caryophylla by using Agrobacterium and an application thereof. This method utilizes the Agrobacterium that does not insert the target gene to infect plant shoots to cause changes in plant genetic traits. An obstacle to the improvement of breeding plant varieties, it is of great value to the genetic improvement of vegetative propagation of carnation flowers with ornamental value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mutagenesis method and application of carnation buds. Background technique [0002] Asexual reproduction refers to asexual reproduction with a part of the plant's vegetative organs and materials such as flower buds, anthers, and female gametophytes. Asexual reproduction does not involve reproductive cells, does not need to go through the fertilization process, and directly forms a new individual from a part of the mother. The genes of the new individual and the mother are exactly the same, and the excellent characteristics of the original variety can be well maintained. However, for plant breeding, it is indeed a disadvantageous factor to keep the characteristics of the original variety well, and it is an obstacle to the improvement of asexually propagated varieties. Therefore, there is an urgent need for a method that can overcome obstacles to the improvement of asexu...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 周旭红刘小莉张洪磊杨晓密陈俊騵石少卿尧雪洲
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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