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Mutagenesis method of tender shoots of dianthus flowers and application of mutagenesis method

A technology of Dianthus and young shoots, applied in the biological field, can solve the problems of certain risks to the health of breeding operators, environmental pollution, improper protection, etc., and achieve the effects of overcoming obstacles to variety improvement, not polluting the environment, and simple operation.

Active Publication Date: 2020-09-22
YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Physical mutagenesis often uses various rays, and improper protection will pose certain risks to the health of breeding operators; chemical mutagenesis uses various chemical reagents, which has certain pollution to the environment

Method used

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  • Mutagenesis method of tender shoots of dianthus flowers and application of mutagenesis method
  • Mutagenesis method of tender shoots of dianthus flowers and application of mutagenesis method
  • Mutagenesis method of tender shoots of dianthus flowers and application of mutagenesis method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Take 10 μL of GV3101 Agrobacterium in an ultra-low temperature refrigerator and put it into 20ml of YEB liquid medium, add rifampicin with a final concentration of 60mg / L, shake and culture at 28°C and 200rpm for 20h, and obtain activated Agrobacterium liquid, Agrobacterium liquid OD 600 The value is 0.3.

[0036] Get 50 shoots of 4 cm in carnation variety 'Lan Guiren', peel off the young leaves to expose the stem tip, prick the wound around the shoot tip with a dissecting needle, then soak the shoots in the bacterium solution of activated GV3101 Agrobacterium, in Shake culture at 26°C and 30rpm in the dark for 16 hours; take out the shoots and wash them with water for 3 times, then immerse them in 400mg / L cefotaxime sodium solution for 4 hours; take out the shoots and wash them with water for 3 times, put in the rooting powder In the substrate, the substrate is made of humus, perlite and coconut shell in a mass ratio of 1:1:1; after 20 days of rooting, the rooted seedl...

Embodiment 2

[0038] Take 20 μL of EHA105 Agrobacterium in an ultra-low temperature refrigerator and put it into 30ml of YEB liquid medium, add rifampicin with a final concentration of 70mg / L, shake and culture at 28°C and 200rpm for 22h, and obtain activated Agrobacterium liquid, Agrobacterium liquid OD 600 The value is 0.32.

[0039]Get 50 shoots of 3 cm in carnation variety 'Fen Lian', peel off the young leaves to expose the stem tip, prick the wound with a dissecting needle around the shoot tip, then soak the shoots in the bacterium solution of activated EHA105 Agrobacterium. Shake culture at 27°C and 40rpm in the dark for 24 hours; take out the shoots and wash them with water for 3 times, then immerse them in 450 mg / L cefotaxime sodium solution for 6 hours; take out the shoots and wash them with water for 3 times, put in the rooting powder In the matrix, the matrix is ​​made of humus, perlite and coconut shell in a mass ratio of 2:2:1. Put the rooted seedlings into the greenhouse for c...

Embodiment 3

[0041] Take 30 μL of LBA4404 Agrobacterium in an ultra-low temperature refrigerator and put it into 40ml of YEB liquid medium, add rifampicin with a final concentration of 65mg / L, shake and culture at 28°C and 200rpm for 24 hours, and obtain activated Agrobacterium liquid, Agrobacterium liquid OD 600 The value is 0.4.

[0042] Take 50 shoots of carnation variety 'Master' 3cm, peel off the young leaves to expose the stem tip, prick the wound with a dissecting needle around the shoot tip, then soak the shoots in the bacterium liquid of activated LBA4404 Agrobacterium, Shake culture at 27°C and 40rpm in the dark for 24 hours; take out the shoots and wash them with water for 3 times, then immerse them in 500 mg / L cefotaxime sodium solution for 8 hours; take out the shoots and wash them with water for 3 times, and add root-growing powder Insert it into the substrate, which is made of humus, perlite and coconut shell in a mass ratio of 1:3:1; 30 days after rooting, put the rooted se...

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Abstract

The invention belongs to the technical field of biology, and particularly relates to a mutagenesis method of tender shoots of dianthus flowers and application of the mutagenesis method. According to the method, agrobacterium with no target gene inserted is used to attach tender shoots of plants to cause change of hereditary characters of plants. The method is simple to operate and large in mutation range, can obtain tender shoot mutants in a very short time, effectively overcomes the obstacles of vegetative propagation plant variety improvement, and has important value for the genetic improvement of the asexual propagation of dianthus flowers with ornamental value.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a mutagenesis method and application of carnation buds. Background technique [0002] Asexual reproduction refers to asexual reproduction with a part of the plant's vegetative organs and materials such as flower buds, anthers, and female gametophytes. Asexual reproduction does not involve reproductive cells, does not need to go through the fertilization process, and directly forms a new individual from a part of the mother. The genes of the new individual and the mother are exactly the same, and the excellent characteristics of the original variety can be well maintained. However, for plant breeding, it is indeed a disadvantageous factor to keep the characteristics of the original variety well, and it is an obstacle to the improvement of asexually propagated varieties. Therefore, there is an urgent need for a method that can overcome obstacles to the improvement of asexu...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 周旭红刘小莉张洪磊杨晓密陈俊騵石少卿尧雪洲
Owner YUNNAN UNIV OF TRADITIONAL CHINESE MEDICINE