Preparation method of allogeneic or xenogeneic tendon and bone tendon complex

An allogeneic and complex technology, applied in prosthesis, medical science, tissue regeneration, etc., can solve problems such as inability to apply clinically, reduce immunogenicity, damage tendon mechanical properties, etc., and achieve complete tendon matrix structure and obvious effect. , the effect of simple operation

CN111686303AInactive Publication Date: 2020-09-22上海亚朋生物技术有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2020-09-22
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to a preparation method of an allogeneic or xenogeneic tendon and bone tendon complex. The preparation method comprises the following steps that: S1: an allogeneic or xenogeneictendon and bone tendon complex material is obtained, and bloodstains on the surfaces of the tendons are cleaned with purified water; S2, supercritical CO2 extraction equipment is started, and the cleaned tendons are put into the supercritical CO2 extraction equipment for extraction; S3, the extracted tendons are subjected to decellularization treatment and grease washing; S4, the purified water isadded into the tendons subjected to oscillation washing, and oscillation washing is carried out in a shaking table at room temperature; S5, the tendons subjected to oscillation washing are trimmed, water on the surfaces of the trimmed tendons is drained off, and vacuum packaging is carried out under aseptic conditions; and S6, the packaged tendons with cobalt-60 under the dosage of 10-30 kGy areirradiated, and sterilization again is carried out so as to obtain the tendons subjected to vacuum packaging. The preparation method has the advantages of convenient processing, high efficiency, environmental protection and safety.
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Description

technical field

[0001] The invention relates to a preparation method of allogeneic or heterogeneous tendon and bone-tendon complex. Background technique

[0002] Cruciate ligament injuries are common in sports injuries and traffic accident injuries. With the development of modern competitive sports and the increase in high-speed vehicle traffic accidents, the injuries of the cruciate ligament of the knee joint are also increasing year by year. The cruciate ligament is an important structure for the stability of the knee joint. After its injury, it can lead to joint pain and joint instability. In the later stage, it often causes joint laxity, joint degeneration, secondary meniscus injury and articular cartilage destruction. In severe cases, it can cause arthropathy waste. Therefore, the treatment after cruciate ligament injury has always been one of the important issues that orthopedic surgeons pay attention to.

[0003] The accepted surgical method for knee cruciate ligame...

Examples

preparation example Construction

[0031] like Figure 1 to Figure 4 Shown: the preparation method of the allogeneic or heterogeneous tendon and bone-tendon complex according to the embodiment of the present invention, specifically comprising the following steps:

[0032] Step S1: Obtain the allogeneic or heterogeneous tendon and bone-tendon composite material, and clean the blood on the surface of the tendon with purified water;

[0033] Step S2: Turn on supercritical CO 2 extraction equipment, the cleaned tendons were placed in the supercritical CO 2 extraction in extraction equipment;

[0034]Step S3: adding 0.02% to 0.05% sodium lauryl sulfate to the tendons extracted in step S2 according to the weight ratio of (1-3):(10-30) for decellularization and oil washing , at the same time, carry out shaking washing at a temperature of 20°C to 50°C, and the time of shaking washing is 0.5 to 2.0h;

[0035] Step S4: add purified water to the tendon after shaking and washing in step S3 according to the ratio of (1-...

Embodiment 1

[0058] The preparation method of the allogeneic or heterogeneous tendon and bone-tendon complex provided in Example 1 of the present invention specifically includes the following steps:

[0059] Step S1: Obtain the allogeneic or heterogeneous tendon and bone-tendon composite material, and clean the blood on the surface of the tendon with purified water;

[0060] Step S2: Turn on supercritical CO 2 extraction equipment, the cleaned tendons were placed in the supercritical CO 2 Extraction in the extraction equipment: the extraction pressure is set to 30Mpa, the extraction temperature is set to 45°C; CO 2 The gas flow rate is set to 20L / h, and the extraction time is set to 1h; entrainer is added for extraction. The entraining agent is peracetic acid.

[0061] Step S3: Add 0.02% sodium lauryl sulfate to the tendon extracted in step S2 at a weight ratio of 1:10 for decellularization and oil washing, and at the same time, carry out at a temperature of 30°C Shock washing, and the...

Embodiment 2

[0066] The preparation method of the allogeneic or heterogeneous tendon and bone-tendon complex provided in Example 2 of the present invention specifically includes the following steps:

[0067] Step S1: Obtain the allogeneic or heterogeneous tendon and bone-tendon composite material, and clean the blood on the surface of the tendon with purified water;

[0068] Step S2: Turn on supercritical CO 2 extraction equipment, the cleaned tendons were placed in the supercritical CO 2 Extraction is carried out in the extraction equipment: the extraction pressure is set to 60Mpa, the extraction temperature is set to 32°C; CO 2 The gas flow rate is set to 20L / h, and the extraction time is set to 5h; entrainer is added for extraction. The entraining agent is hydrogen peroxide.

[0069] Step S3: add 0.04% sodium lauryl sulfate to the tendon extracted in step S2 at a weight ratio of 1:30 for decellularization and oil washing, and at the same time, carry out at a temperature of 37°C Shoc...