Unlock instant, AI-driven research and patent intelligence for your innovation.

Design method of long-chain RNA specific probe

An RNA probe and specific technology, applied in the field of design of long-chain RNA-specific probes, can solve the problem that there is no long-chain RNA expression abundance gene chip and method, and achieve the effect of high-throughput detection

Pending Publication Date: 2020-09-22
SHANGHAI BIOCHIP
View PDF8 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After more than ten years of development, gene chip technology has been continuously improved and matured, and has been widely used in various fields of life sciences. Gene chip and method for expression abundance of long-chain RNA

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Design method of long-chain RNA specific probe
  • Design method of long-chain RNA specific probe
  • Design method of long-chain RNA specific probe

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0065] In the present invention, the term "long-chain RNA" includes mRNA, long-chain non-coding RNA and circRNA, etc.

[0066] The term "probe" refers to a DNA or RNA nucleic acid sequence whose sequence is known and complementary to the target gene.

[0067] The term "specific probe" refers to a probe with strong specificity and no mutual interference between the probes of various long-chain RNAs when multiple long-chain RNAs are detected simultaneously.

[0068] The term "full-length target sequence" refers to the full-length sequence of the RNA where the fragment recognized by the probe is located, and the sequence of the RNA given in the sequence database is generally the sequence of the sense strand of DNA.

[0069] The term "similarity", the DNA sequence is composed of four bases, A, T, C, and G, and various existing scoring schemes (such as matching scoring matrix) are used to score the similarity of the bases of the two sequences. The resulting score is the similarity...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to the technical field of biology, in particular to a design method of a long-chain RNA specific probe. The long-chain RNA specific probe can be used for simultaneously detectingthe expression abundance or differential expression of at least two long-chain RNAs in mRNA, circular RNA or long-chain non-coding RNA. The long-chain RNA specific probe obtained through the design method is high in sensitivity and specificity, and mRNA expression abundance of regulatory RNA molecules such as circular RNA and long-chain non-coding RNA of a trace sample and expression protein canbe detected simultaneously, rapidly and in a high-throughput mode.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for designing a long-chain RNA specific probe. · Background technique [0002] Circular RNAs (circRNAs) are a class of novel RNA molecules that are characterized by covalently closed loops and widely exist in eukaryotes. circRNAs are derived from the exon or intron regions of genes and are abundant in mammalian cells. The formation of circRNAs is different from the standard splicing pattern of linear RNAs, which are spliced ​​by the 5' end of the donor exon and the 3' end of the acceptor exon end-to-end, forming a backsplicing site (backsplicing). The existing circRNA formation models mainly include the following types (see figure 1 shown): [0003] (1) "Lariat-driven circularization" or "exon skipping", such as figure 1 As shown in A; [0004] (2) "Intron-pairing-driven circularization" or "direct backsplicing", such as figure 1 As shown in B; [0005] (3) Formation...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): G16B25/20C12Q1/6837
CPCG16B25/20C12Q1/6837C12Q2525/307C12Q2565/519
Inventor 张晓娜曹群发庞盼盼韩峻松
Owner SHANGHAI BIOCHIP
Features
  • R&D
  • Intellectual Property
  • Life Sciences
  • Materials
  • Tech Scout
Why Patsnap Eureka
  • Unparalleled Data Quality
  • Higher Quality Content
  • 60% Fewer Hallucinations
Social media
Patsnap Eureka Blog
Learn More

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2025 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com