Antimicrobial peptide-containing feed prepared based on solid-state fermentation of bacillus, and preparation process and fermentation strain of feed

Abstract

The invention provides an antimicrobial peptide-containing feed additive based on solid-state fermentation of bacillus and a process. Bacillus (CGMCC19541) is selected as a main substrate in the invention, and antimicrobial peptides and proteases are produced through solid-state fermentation; and the process conditions for the production of antimicrobial peptides by solid-state fermentation are: 1-5% of sodium glutamate addition amount, 0.1-0.5% of potassium chloride addition amount, 0.1-0.6% of magnesium sulfate addition amount. In addition, it is also necessary to add 0.1-0.3% of dipotassiumhydrogen phosphate, and 1-4% of glucose; material-to-liquid ratio is 1:0.9, inoculation amount is 3-8%, fermentation temperature is 37-45 DEG C, and fermentation time is 24-72 hours; and FeS additionamount is 0.5-3 mg / kg, CuS addition amount is 1.0-3.0 mg / kg, MnS addition amount is 0.2-0.8 mg / kg, and ZnS addition amount is 0.2-0.8 mg / kg. The proteases and new antimicrobial lipopeptide protease which are produced by fermentation can effectively remove anti-nutritional factors such as antigen proteins in soybean meal, and prevent and treat the diarrhea caused by the indigestion of piglets dueto the anti-nutritional factors; and the new antimicrobial lipopeptide can exhibit a good inhibitory effect on intestinal pathogens, so as to improve the intestinal micro flora of piglets.

Description

technical field [0001] The invention relates to the technical field of feed, in particular to an antimicrobial peptide-containing feed prepared based on solid-state fermentation of bacillus, a preparation process and a fermentation strain thereof. Background technique [0002] One of the biggest problems faced by the breeding industry is that growing animals suffer from various diseases. The key to animal health is to enhance their disease resistance. The livestock and poultry farming industry often uses antibiotics as a growth-promoting factor, which has also made a great contribution to the farming industry. However, the long-term and large-scale use of antibiotics has destroyed the micro-ecological balance of the animal body, leading to the emergence of drug-resistant bacteria and bacterial mutations, and is likely to cause endogenous infection or secondary infection in animals. At the same time, when the drug residues in meat products occur, directly threatening human h...

AI Technical Summary

Problems solved by technology

However, the long-term and large-scale use of antibiotics has destroyed the micro-ecological balance of the animal body, leading to the emergence of drug-resistant bacteria and bacterial mutations, and is likely to cause endogenous infection or secondary infection in animals. At the same time, when the drug residues in meat products Occurs and directly threatens human health

Images