Microfluidic device and system for multi-channel parallel detection of cell deformability
A cell deformation, multi-channel technology, applied in laboratory containers, specific-purpose bioreactors/fermenters, biochemical instruments, etc., can solve the problems of limiting detection throughput, time-consuming, etc., and improve detection throughput. , High biological activity, obvious effect of cell deformation
Active Publication Date: 2022-03-08
SOUTHEAST UNIV
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[0004] Purpose of the invention: The purpose of the invention is to provide a microfluidic device for multi-channel parallel detection of cell deformability, which solves the problem that the existing detection needs to capture and fix the cells, which takes a long time and limits the detection throughput
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[0022] The present invention will be further described below in conjunction with the accompanying drawings.
[0023] Such as figure 1 As shown, the present invention discloses a microfluidic device for multi-channel parallel detection of cell deformability, including a sample inlet 1, a filter screen 2, a spiral flow channel 3, a Y-shaped outlet channel 4, a first blood cell outlet 5, Deformability detection flow channel 6, circulating tumor cells and white blood cells of similar size to the second outlet 7.
[0024] The upper end of the integrated device is provided with a sample inlet 1, and the sample liquid is pushed by a syringe pump to inject the sample liquid into the device from the sample inlet 1, and a filter screen 2 is set at the sample inlet 1, and when the sample liquid flows through the filter screen 2, large particles of impurities are eliminated intercepted, thereby avoiding blockage of the flow channel of the device. The bottom of the sample inlet 1 is conn...
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The invention discloses a microfluidic device and system for multi-channel parallel detection of cell deformability, including a sample inlet, a spiral flow channel, a Y-shaped double outlet, and a deformability detection flow channel; the spiral flow is connected under the sample inlet One end of the channel, the other end of the spiral flow channel is connected to the Y-shaped outlet channel, the first branch of the Y-shaped outlet channel is connected to the first outlet, and the second branch is connected to the deformability detection flow channel, and the deformability detection flow channel A plurality of parallel passages are provided, and the deformability detection flow passage communicates with the second outlet. The invention integrates inertial sorting, effectively realizes high-throughput sorting of cells in whole blood; uses fluid pressure to realize cell deformation, and multi-channel parallel detection realizes high-throughput detection of cell deformability, and cells still have high throughput after detection. activity, thereby distinguishing remaining white blood cells from cancer cells.
Description
technical field [0001] The invention relates to a microfluidic device and system, in particular to a microfluidic device and system for multi-channel parallel detection of cell deformability. Background technique [0002] In recent years, the method of sorting and enriching circulating tumor cells based on microfluidic technology has received extensive attention and made breakthroughs. However, the identification and characterization of sorted circulating tumor cells usually still use traditional analytical methods, such as immunocytology, flow cytometry, and nucleic acid detection techniques. These methods all focus on biomolecular markers, which not only affect cell viability, but also fail to detect circulating tumor cells that do not express specific molecular markers. For example, some tumor cells may undergo epithelial-mesenchymal transition and be lost during metastasis. Epithelial cell markers. In addition, these methods also have common shortcomings such as comple...
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IPC IPC(8): B01L3/00C12M1/12C12M1/34C12M1/00
CPCB01L3/502761B01L3/502753C12M23/16C12M33/14C12M41/46C12M47/04
Inventor 项楠张孝哲倪中华
Owner SOUTHEAST UNIV