Microfluidic device and microfluidic system for multi-channel parallel detection of cell deformability

Abstract

The invention discloses a micro-fluidic device and system for multi-channel parallel detection of cell deformability. The micro-fluidic device comprises a sample inlet, a spiral flow channel, a Y-shaped two-way outlet and a deformability detection flow channel, the lower part of the sample inlet is communicated with one end of a spiral runner; the other end of the spiral flow channel is communicated with a Y-shaped outlet channel; a first branch of the Y-shaped two-way outlet is communicated with a first outlet and a second branch of the same is communicated with a deformability detection flowchannel; a plurality of parallel channels are arranged in the deformability detection flow channel, and the deformability detection flow channel is communicated with a second outlet. Inertia sortingis integrated, and high-throughput sorting of cells in whole blood is effectively achieved; cell deformation is achieved through fluid pressure, multi-channel parallel detection is achieved, high-throughput detection of cell deformability is achieved, detected cells still have high activity, and therefore, residual leukocytes and cancer cells are identified.

Description

technical field [0001] The invention relates to a microfluidic device and system, in particular to a microfluidic device and system for multi-channel parallel detection of cell deformability. Background technique [0002] In recent years, the method of sorting and enriching circulating tumor cells based on microfluidic technology has received extensive attention and made breakthroughs. However, the identification and characterization of sorted circulating tumor cells usually still use traditional analytical methods, such as immunocytology, flow cytometry, and nucleic acid detection techniques. These methods all focus on biomolecular markers, which not only affect cell viability, but also fail to detect circulating tumor cells that do not express specific molecular markers. For example, some tumor cells may undergo epithelial-mesenchymal transition and be lost during metastasis. Epithelial cell markers. In addition, these methods also have common shortcomings such as comple...

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Problems solved by technology

[0004] Purpose of the invention: The purpose of the invention is to provide a microfluidic device for multi-channel parallel detection of cell deformability, which solves the problem that the existing detection needs to capture and fix the cells, which takes a long time and limits the detection throughput

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