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KASP primer group related to wheat grain weight and application thereof

A technology of wheat kernels and primer sets, used in recombinant DNA technology, biochemical equipment and methods, and the determination/inspection of microorganisms

Active Publication Date: 2020-10-13
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no report on the molecular markers related to the grain weight of Yangmai 158 and Ningmai 9

Method used

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  • KASP primer group related to wheat grain weight and application thereof
  • KASP primer group related to wheat grain weight and application thereof
  • KASP primer group related to wheat grain weight and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1 Screening thousand-grain weight marker interval

[0034] A recombinant inbred line (RIL) population (F 2:8 ), including 282 families. The RIL population and its parents were planted in Liuhe Base of Jiangsu Academy of Agricultural Sciences in two consecutive growing seasons from 2016 to 2017 and 2017 to 2018.

[0035] Augmented design (see literature "Lan C, Zhang Y, Herrera-Foessel S A, Basnet B R, Huerta-Espino J, Lagudah E S, Singh R P. Identification and characterization of pleiotropic and co-located resistance loci to leaf rust and stripe rust inbread wheat cultivar Sujata. Theoretical and Applied Genetics, 2015, 128:549-561"), 2 parents and 30 randomly selected materials were planted for 2 repetitions, and the rest of the materials were planted for 1 repetition, planted in three rows of plots, 60 grains per row , row length 1.6m, row spacing 0.25m, conventional cultivation management. The grains were harvested after they matured, and the thousand-grai...

Embodiment 2

[0038] Example 2 Screening and validation of KASP molecular markers

[0039] In order to better utilize the Qtkw-7A locus screened in Example 1 for breeding, a KASP marker suitable for high-throughput genotyping was developed based on its interval marker sequence. PCR amplification primers were designed according to SNP sites and flanking sequences, and KASP molecular markers were developed.

[0040] KASP primers were designed using Polymarker (http: / / www.polymarker.info / ), and the primers were synthesized by Sangon Bioengineering (Shanghai) Co., Ltd. Two SNP-specific primers (F1 / F2) and one universal primer (R) were designed for each marker, and a specific sequence (SEQ ID NO.10:5'-GAAGGTGACCAAGTTCATGCT-3 '), the front of the F2 primer added a specific sequence capable of combining with HEX fluorescence (SEQ ID NO.11:5'-GAAGGTCGGAGTCAACGGATT-3'). Three SNP markers with lower homology were selected from multiple SNP sites in this marker interval for primer design as shown in...

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Abstract

The invention discloses a KASP primer group related to wheat grain weight and an application thereof. The primer group comprises an F1 primer with a nucleotide sequence as shown in SEQ ID NO.1, an F2primer with a nucleotide sequence as shown in SEQ ID NO.2 and a universal primer with a nucleotide sequence as shown in SEQ ID NO.3; and the KASP primer group can be used for preparing a PCR detectionsystem for wheat grain weight screening, and can be widely applied to the field of wheat breeding.

Description

technical field [0001] The application relates to the field of wheat breeding, especially a set of KASP marker primer sets related to wheat grain weight and its application. Background technique [0002] Grain weight is an important factor affecting wheat yield, which is mainly controlled by additive effects, and heritability in wheat yield factors can reach 59-80% (Xiao Shihe, He Zhonghu. "Improvement of wheat yield potential and quality", see : Zhuang Qiaosheng. "Chinese Wheat Variety Improvement and Pedigree Analysis". Beijing: China Agricultural Press, 2003.pp 497-542). Wheat grain weight is a quantitative trait with continuous variation and is regulated by microgenes (Zhang L Y, Liu D C, Guo X L, Yang W L, Sun J Z, Wang D W, Zhang A. Genomic Distribution of Quantitative Trait Loci for Yield and Yield-Related Traits in Common Wheat. Journal of Integrative Plant Biology, 2010, 52:996-1007). [0003] Mason et al. investigated wheat yield traits at different sowing dates,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/13C12Q2600/156C12Q2531/113C12Q2563/107
Inventor 姜朋马鸿翔张旭吴磊何漪
Owner JIANGSU ACAD OF AGRI SCI