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Bacillus velezensis and application thereof

A technology of Bacillus Velez and Bacillus, applied in the field of microbiology, can solve the problems that the pathogenicity of peach fruit has not been reported.

Active Publication Date: 2020-10-16
ZHONGKAI UNIV OF AGRI & ENG
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It is generally believed in the industry that the rot caused by these fungi is an important factor limiting the freshness of peaches after harvest, but there is no report on the pathogenicity of bacteria to peach fruits

Method used

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  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof
  • Bacillus velezensis and application thereof

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Embodiment Construction

[0025] The technical solutions of the present invention will be further described in detail below with reference to the drawings and specific embodiments of the specification. These embodiments are only for explanatory purposes and do not limit the present invention in any form.

[0026] Isolation and identification of a strain

[0027] The screening method of the present invention for postharvest spoilage bacteria of olecranon peach is as follows:

[0028] 1. Collecting olecranon

[0029] During the middle period of the olecranon peach fruit period, randomly collected olecranon peach spoilage fruits from different orchards in Lianping area of ​​Heyuan City, Guangdong Province (one of the main producing areas of olecranon peach), with no less than 30 per park. Seal with a sterile bag and transport to the laboratory at room temperature within 24h.

[0030] 2. Cultivation and isolation of spoilage bacteria

[0031] Using the tissue culture method, a sterile knife was used to cut out small...

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Abstract

The invention provides Bacillus velezensis ZK1. The preservation number is CGMCCC NO. 17209. The strain isolated in a laboratory is taken as an example, putrefaction of Bacillus velezensis on peach fruit is firstly raised, and metabonomics detects that the strain produces toxic substances after infecting Yingzui peach. Bacillus velezensis ZK1 as a standard bacterium can have an important effect onquality control of peach fruit after harvest.

Description

Technical field [0001] The invention belongs to the technical field of microorganisms, and specifically relates to a strain of Bacillus Velez. Background technique [0002] Peach (Amygdaluspersica Linn) belongs to the Rosaceae family and has been cultivated in China for four thousand years. In 2017, the total cultivated area and total output of peaches in China accounted for 51.16% and 57.95% of the world's total peaches (FAOSTAST, 2017). There are many peach varieties selected and cultivated in China, covering many provinces such as Shandong, Shaanxi, Yunnan, Jiangsu and Guangdong. They are an important part of the local fruit tree planting industry. Fresh food is one of the main sales forms of peaches in the Chinese market. [0003] The peaches used for fresh food need to undergo a period of storage and transportation after harvest, but in this process, the peaches often rot in batches. Preservation and preservation of peaches after harvest has always been a problem for the fo...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12Q1/04C12Q1/06C12R1/07
CPCC12N1/20C12Q1/04C12Q1/06C12N1/205C12R2001/07
Inventor 余倩刘悦刘功良曾瑶英陈少先
Owner ZHONGKAI UNIV OF AGRI & ENG
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