Method for applying bacillus bacterium fluid in sea cucumber juvenile culturing
A bacillus and culture method technology, which is applied in the field of sea cucumber breeding and bacillus liquid, can solve the problems of small number of bacteria in the liquid, few counterpart strains, low cost performance of aquatic probiotic products, etc.
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Embodiment 1
[0107] Step 1: The temperature in the lactic acid bacteria cultivation room is required to be controllable, and an ultraviolet lamp is installed according to the room space, with an average of 2w per cubic meter;
[0108] Step 2: Use an open plastic barrel as a lactic acid bacteria culture container, cut a transparent plastic cloth according to the size of the mouth of the barrel for sealing, cover the mouth of the barrel with the plastic cloth, and tie it tightly around the mouth. The center of the plastic cloth is perforated to facilitate the passage of the inflatable tube, the contact between the plastic cloth and the trachea is tied tightly with a rope, and all items are sterilized by ultraviolet light for 1 hour before inoculation;
[0109] Step 3: Put the beaker, measuring cylinder and other tools used in the expansion process into water and boil for 10 minutes for disinfection;
[0110] Step 4: The inflatable pipeline is filtered through a precision filtration system; ...
Embodiment 2
[0125] A simple method for cultivating probiotics suitable for aquaculture, the operation of the method is as follows:
[0126] Step 1: The temperature in the subtilis spore culture room is required to be controllable, and an ultraviolet lamp is installed according to the room space, with an average of 3w per cubic meter;
[0127] Step 2: Use an open plastic barrel as a subtilis spore culture container, cut a transparent plastic cloth according to the size of the mouth of the barrel for sealing, cover the mouth of the barrel with the plastic cloth, and tie the surroundings tightly with ropes. The center of the plastic cloth is perforated to facilitate the passage of the inflatable tube, the contact between the plastic cloth and the trachea is tied tightly with a rope, and all items are sterilized by ultraviolet light for 2 hours before inoculation;
[0128] Step 3: Put the beaker, measuring cylinder and other tools used in the expansion process into water and boil for 15 minut...
Embodiment 3
[0145] A simple method for cultivating probiotics suitable for aquaculture, the operation of the method is as follows:
[0146] Step 1: The temperature in the lactic acid bacteria cultivation room is required to be controllable, and an ultraviolet lamp is installed according to the room space, with an average of 5w per cubic meter;
[0147] Step 2: Use an open plastic barrel as a probiotic culture container, cut a transparent plastic cloth according to the size of the mouth of the barrel for sealing, cover the mouth of the barrel with the plastic cloth, and tie it tightly around the mouth. The center of the plastic cloth is perforated to facilitate the passage of the inflatable tube, and the contact between the plastic cloth and the trachea is tied tightly with a rope. Before inoculation, all items are sterilized by ultraviolet light for 3 hours;
[0148] Step 3: Put the beaker, measuring cylinder and other tools used in the expansion process into water and boil for 30 minutes...
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