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Animal model of mouse atherosclerosis and establishment method thereof

An atherosclerosis and animal model technology, applied in the field of basic medicine, can solve the problems of time-consuming and high cost of modeling

Pending Publication Date: 2020-10-23
XIAN MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The first object of the present invention is to provide an animal model of mouse atherosclerosis, which solves the problems of long time-consuming and high cost of manpower and material resources in the prior art for mouse atherosclerosis modeling

Method used

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  • Animal model of mouse atherosclerosis and establishment method thereof
  • Animal model of mouse atherosclerosis and establishment method thereof
  • Animal model of mouse atherosclerosis and establishment method thereof

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Experimental program
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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with the accompanying drawings and specific embodiments.

[0026] A mouse atherosclerosis animal model of the present invention can induce the expression of mouse low-density lipoprotein receptor degradation protein (IDOL) through the systemic high expression of adenovirus, and IDOL can degrade the low-density lipoprotein receptor, so that free Increased low-density lipoproteins in mice can easily cause atherosclerosis in mice. The animal model prepared by the invention has the advantages of obvious phenotype and shortened modeling time.

[0027] A method for establishing a mouse atherosclerosis animal model of the present invention specifically comprises the following steps:

[0028] Step 1, construct an adenovirus expression vector, such as figure 1 Shown is the flow chart of the construction of the adenovirus overexpression vector of the present invention.

[0029] Step 1.1. Use Hind Ⅲ and Sal Ⅰ...

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Abstract

The invention discloses an animal model of mouse atherosclerosis. The animal model can be obtained by inducing a mouse low density lipoprotein receptor to degrade the expression of protein through thesystematic high expression of adenovirus. An establishment method of the animal model is also disclosed. Through the systematic high expression of IDOL by the adenovirus, the course of mouse atherosclerosis model establishment can be influenced; and the technology can shorten the time for establishing the model and save animal feeding costs and experimental time.

Description

technical field [0001] The invention belongs to the technical field of basic medicine, and in particular relates to a mouse atherosclerosis animal model, and also relates to a method for establishing the above-mentioned mouse atherosclerosis animal model. Background technique [0002] Atherosclerosis is the number one killer of human death in the world, and one of the major difficulties in current atherosclerosis research is the formation of animal models of atherosclerosis. The existing mouse model of atherosclerosis is based on gene knockout and can only be completed by feeding high-fat diet for about 12 weeks, which consumes a relatively high cost of time and material resources. This patented technology uses adenovirus to systematically Expressing IDOL can affect the process of atherosclerosis modeling in mice. This technology can speed up the modeling time, reduce the modeling time to 6 weeks, and save animal feeding costs and experimental time. Contents of the inven...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/861C12N15/66A01K67/027
CPCC12N15/86C12N15/66C07K14/47A01K67/0278A01K2207/15A01K2207/25A01K2217/072A01K2227/105A01K2267/0375C12N2710/10043
Inventor 余琦张云婷
Owner XIAN MEDICAL UNIV