A method for determination of flavonoids in Xintahua by one-test-multiple-assessment method and its application

A technique for flavonoids and flavonoids of rhizoma rhododendron, which is applied to the determination of the content of flavonoids in rhododendron rhododendron and its application field by one-test-multiple-evaluation method, and achieves the effects of cost saving, simple operation and good reproducibility.

Active Publication Date: 2022-07-08
XINJIANG MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a method and its application for the determination of the flavonoid components of Xintahua by using the method of one test and multiple evaluations. The present invention provides a highly practical, Simple and cost-effective detection method for the four components of Xintahua rutin, apigenin, luteolin and mongoside

Method used

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  • A method for determination of flavonoids in Xintahua by one-test-multiple-assessment method and its application
  • A method for determination of flavonoids in Xintahua by one-test-multiple-assessment method and its application
  • A method for determination of flavonoids in Xintahua by one-test-multiple-assessment method and its application

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Experimental program
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Effect test

Embodiment 1

[0046] Embodiment 1: the method for measuring the content of flavonoids in Xintahua by one-measurement and multi-evaluation method

[0047] The one-test-multi-evaluation method was used to determine the composition of Xintahua, and the cheap and easily available rutin was used as an internal reference to conduct one-test-multiple-evaluation. The relative correction factor between the glycosides, the content of rutin, apigenin, luteolin and montiflorin in Xintahua was calculated by the correction factor, and the liquid chromatography was used to determine the specific method as follows:

[0048] (1) Material preparation: Grind the new tower flower of the test product into powder and pass it through No. 2 sieve for use;

[0049] (2) Preparation of reference substance solution: rutin, luteolin, apigenin, and montiflorin are all reference substances accurately weighed, dissolved in methanol, and made into a reference solution with a concentration of 1.0 mg / ml; respectively take th...

Embodiment 2

[0057] Embodiment 2: the method for measuring the content of flavonoids in Xintahua by one-measurement and multi-evaluation method

[0058] Based on Example 1, take the mixed reference solution in step (2), inject 1, 2, 4, 6, 8, and 10 μL of samples respectively for determination, and use rutin as the internal standard to calculate luteolin, montanulin, and apigenin respectively. The relative correction factor fR, using Agilent1220, Shimadzu LC-20AT type, Waterse2695-2489 type 3 high performance liquid chromatography systems, Hypersil ODS-2 (C18) (4.6mm × 150mm, 5μm, batch number: 12190) chromatographic column, Hypersil ODS-2(C18) (4.6mm×250mm, 5μm, Lot No.: 12190) column, Hypersil ODS-2(C18) (4.6mm×150mm, 5μm Lot No.: 10765) to investigate the effect of the column on fR, luteolin The relative standard deviation RSD of the four components, rutin, rutin, apigenin and apigenin, is less than 2%, and the relative correction factor fR of rutin, rutin and apigenin is determined as t...

Embodiment 3

[0059] Embodiment 3: Qualitative analysis of the chemical composition of the effective part of the total flavonoids of Aromatic Xintahua

[0060] (1) Establishment of HPLC analysis method

[0061] ①Inspection of flow velocity

[0062] Chromatography system 1: ELITE Hypersil ODS2 (4.6mm×150mm), mobile phase A is aqueous phase: 0.1% phosphoric acid-water, mobile phase B is organic phase: methanol, wavelength 360nm, gradient elution: 0.01→55min, organic phase : 25%→28%, 55.01→70min, 28%→35%, 70.01→85min, 38%, stop at 85.1min, column temperature 26°C. The flow rate was 0.9 mL / min. The results are attached figure 1 shown. The results show that the attached figure 1 It can be seen that, using the gradient elution system with a flow rate of 0.9 mL / min, some chromatographic peaks are not effectively separated, and the resolution is poor, and the chromatographic conditions need to be further adjusted.

[0063] Chromatography system 2: ELITE Hypersil ODS2 (4.6mm×150mm), mobile p...

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Abstract

The invention relates to a method for one measurement and multiple evaluations of the effective parts of total flavonoids of Xintahua. The relative correction factor between anthocyanins was calculated by the correction factor to calculate the contents of Brassin, luteolin, and montiflorin in Xintahua, and determined by liquid chromatography. The detection method of the invention has the advantages of high practicability, simple operation and cost saving, makes up for the deficiency of only measuring the content of a single component of rutin, and can effectively detect four components of rutin, apigenin, luteolin and montanulin in Xintahua. At the same time, the quality control of the effective parts of the total flavonoids of Xintahua was further improved.

Description

technical field [0001] The invention relates to a method for analyzing and detecting a drug, in particular to a method for determining the content of flavonoids in Xinta flower by adopting a one-measurement-multiple-evaluation method. Background technique [0002] Xinta flower is a commonly used medicinal material in Uyghur medicine. Its Latin name (Ziziphorabungeana. Juz.) According to the records of "Xinjiang Flora" and "Xinjiang Medicinal Flora", Xinta flower is widely grown in the Tianshan area, Xinjiang and Central Asia, Kazakhstan, Kyrgyzstan and other places have grown. Uyghur folks take Qileicaojian decoction orally to relieve symptoms such as chest tightness and dizziness. Among them, flavonoids have a wide range of pharmacological activities and nutritional and health care effects. Studies have shown that their flavonoids can treat myocardial ischemia, increase the activities of superoxide dismutase and glutathione, and reduce malondialdehyde. It can eliminate ox...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/86
CPCG01N30/02G01N30/06G01N30/34G01N30/36G01N30/74G01N30/8679
Inventor 刘宏炳燕雪花马昕张建新孟军董文杰安冬青
Owner XINJIANG MEDICAL UNIV
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