Application of the combination of compound and vitamin E in the preparation of anti-aging medicine or cosmetics
A compound and vitamin technology, applied in the field of medicine, can solve the problems of prolongation, few opportunities to choose, and insufficient variety and types of medicines, etc., and achieve the effect of preventing cell aging, good commercial value, and good antioxidant effect
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Embodiment 1
[0028] The preparation of embodiment 1 formula (I) compound
[0029] Dissolve 0.73ml of 30% H at 0°C 2 o 2 The solution was added to a solution of 1 g of 4-bromoanisole in 5 ml of acetic acid. The mixture was stirred at RT for 12 h and worked up with 10% NaOH solution. The aqueous phase was extracted with ethyl acetate. The organic phase was washed with saturated NaCl solution, washed with Na 2 SO 4dry. Removal of solvent afforded 0.99 g of crude A. 223 mg of sodium azide was added dropwise to a solution of 900 mg of product A in 18 ml of chloroform at 0°C. The mixture was stirred at 0 °C for 12 h and ice water was added. Conventional workup afforded 592.6 mg of product B. 253 mg 3-hydroxy-2-oxo-pyrrolidine-3-carboxylic acid-3-chloro-5-fluoro-benzylamide, 359 mg potassium carbonate, 382 mg N',N'-dimethyl-ethane-1,2 -Diamine and 330 mg of copper iodide were added successively from a solution of 200 mg of product B prepared in the above step in 12 ml of degassed dioxan...
Embodiment 2
[0031] Effect of Example 2 Compounds on ROS Expression in Human Epidermal Keratinocytes
[0032] (1) Take human epidermal keratinocytes in the logarithmic growth phase (Product No. 2110, Sciencell), and adjust the density to 5×10 5 / mL, add to 6-well plate (100μL / well), 37℃, 5%CO 2 Adhesive culture.
[0033] (2) The compound prepared in Example 1 was diluted to a series of concentrations: 1, 10, 50 μg / mL, and quercetin was used as a positive control with a concentration of 50 μg / mL. Serum-free medium was used as a negative control group.
[0034] (3) After 24 hours of attachment, the compound was added. After incubation for 12 h, the original medium was discarded, and serum-free medium containing DCFH-DA (20 mM) was added to each well, except for the blank group.
[0035] (4) Using a multi-functional microplate reader, measure the fluorescence value immediately, the excitation wavelength (485nm), the emission wavelength (538nm), at 37°C, detect every 5min, and the detectio...
Embodiment 3
[0037] The inhibitory effect of embodiment 3 compound on MAPK pathway
[0038] Human epidermal keratinocytes were cultured, and the cells were collected at a density of 5×10 5 individual / mL. First, human epidermal keratinocytes were inoculated into Transwell cell chambers (2 mL / well). Add PBS (2mL / well) and select the irradiation dose. The irradiation dose is 100μj / cm 2 Irradiate for 2 min. After the irradiation, the expression level of p38MAPK was partially detected. Concentration of the compound was added partly, the chamber in the Transwell plate was placed in a 6-well plate with HFF cells, and after incubation for 24 hours, the cells were taken to measure the expression level of p38MAPK. Normal non-irradiated epidermal keratinocytes were used as a negative control, and the results were as follows figure 2 shown. from figure 2 It can be seen that 50 μg / mL compound can significantly reduce the MAPK-related factor p38MAPK in human epidermal keratinocytes. Compared w...
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