Electrochemical method for detecting uric acid and urate oxidase and application thereof

A uric acid oxidase and electrochemical technology, which is applied in the field of functional biomaterials and biosensing, can solve the problems of analysis and detection that have not been reported yet, and achieve the effect of less reagent consumption, low cost and accurate results

Active Publication Date: 2020-11-03
NINGBO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been many reports on the interaction between GSH and metal ions, but the use of biometallic complexes formed by GSH and metal ions for the analysis and detection of uric acid and urate oxidase has not been reported, and it has great potential

Method used

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  • Electrochemical method for detecting uric acid and urate oxidase and application thereof
  • Electrochemical method for detecting uric acid and urate oxidase and application thereof
  • Electrochemical method for detecting uric acid and urate oxidase and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Preparation of Example 1 GSH-Ag(I) Complex

[0031] Take 10 μL of 10 mM silver nitrate aqueous solution and 10 μL of 10 mM glutathione aqueous solution in turn, mix well and add phosphate buffer solution (10 mM, pH 7.0, Na 2 HPO 4 / NaH 2 PO 4 ) into a 100 μL solution, and the solution was shaken slowly at 30° C. for 12 minutes to obtain the GSH-Ag(I) complex. Before use, the complex solution was mixed with 100 μL 0.02% wtNafion solution and allowed to stand for 80 minutes.

Embodiment 2

[0032] The preparation of embodiment 2 electrochemical biosensors

[0033] a. First, the glassy carbon electrode (GCE, 3mm in diameter) is polished on the suede with Al2O3 powder with a particle size of 0.3 μm, 0.1 μm, and 0.05 μm for 2 minutes. After polishing, the electrode is placed in an ultrasonic cleaner for use ultrasonic cleaning in ultrapure water for 2 min, and then 2 Blow dry to obtain a bare glassy carbon electrode, denoted as GCE;

[0034] b. Using cyclic voltammetry, set the potential range to -1.2 ~ 0.5V, scan rate 10mV / s, electrodeposit 1.2mg / mL graphene dispersion (GO) onto the bare glassy carbon electrode to obtain GO / GCE; then use Take 4 μL of the solution in Example 1 and apply it dropwise on GO / GCE, let it stand at room temperature for 30 minutes, and wash the electrode slowly with ultrapure water, which is recorded as GSH-Ag(I) / GO / GCE.

[0035] Detect the electrochemical response of the above electrodes to PBS (0.1M, pH 7.0) electrolyte solution, add 0....

Embodiment 3

[0036] Embodiment 3 feasibility experiment

[0037] According to the sensor preparation steps of the above-mentioned embodiment 1 and embodiment 2, the reaction solution with a total volume of 2mL includes UA (0.001, 0.01, 0.1mM), UOx (500U / L), phosphate buffer solution (Na 2 HPO 4 / NaH 2 PO 4 , 0.1M, pH 8.0), reacted at 37°C for 3min, and then used for the electrochemical response detection of GSH-Ag(I) / GO / GCE. The result is as figure 2 A, It can be seen that GSH-Ag(I) / GO / GCE has a good electrochemical response to different concentrations of UA, which can be applied to the detection of UA.

[0038] Subsequently, according to the sensor preparation steps of the above-mentioned embodiment 1 and embodiment 2, the reaction solution with a total volume of 2mL includes UA (1mM), UOx (500U / L), phosphate buffer solution (Na 2 HPO 4 / NaH 2 PO 4 , 0.1M, pH 8.0), reacted at 37°C for 3min, and then used for the electrochemical response detection of GSH-Ag(I) / GO / GCE. The result ...

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Abstract

The invention discloses a preparation method of an electrochemical sensor based on silver ions and glutathione and application thereof, and the preparation method comprises the following specific steps: firstly, plating a clean bare glassy carbon electrode with a graphene oxide dispersion liquid by an electrochemical method, and synthesizing a GSH-Ag (I) compound by utilizing the interaction between GSH and Ag (I); before use, uniformly mixing the compound solution with 100 [mu] L of a 0.02% wt Nafion solution, allowing the mixture to stand for 80 min, dripping the mixture on the surface of agraphene modified electrode to prepare GSH-Ag (I)/GO/GCE, wherein the electrode has strong electrocatalytic response to H2O2. catalyzing UA by UOx to generate H2O2, fixing the concentration of UOx orUA respectively, and achieving high-sensitivity detection of UA or UOx by taking the response of the compound to H2O2 as signal output. The electrochemical sensor has the advantages of good specificity, high sensitivity, high detection speed, accurate and reliable result and low cost.

Description

technical field [0001] The invention relates to an electrochemical method and its application, in particular to the preparation of an electrochemical sensor based on a biometal complex and its application in the analysis and sensing of uric acid and uric acid oxidase, belonging to the technical field of functional biomaterials and biosensing . Background technique [0002] Uric acid (UA, 2,4,6-trihydroxypurine) is mainly decomposed from nucleic acid and other purine compounds decomposed by cell metabolism and purine in food through the action of enzymes. It is the final product of purine derivatives in human metabolism , The normal level of UA in serum is 240-520μM, and the normal level of UA in urine excretion is 1.4-4.4mM. Studies have found that changes in uric acid levels in the human body are closely related to many clinical cardiovascular diseases and metabolic diseases, such as gout, obesity, hyperuricemia and Lesch-Nyhan syndrome, so uric acid can be used as a valua...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/416G01N27/327G01N27/30
CPCG01N27/416G01N27/3275G01N27/308
Inventor 张青青胡宇芳詹甜玉
Owner NINGBO UNIV
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