A kind of fibrocartilage glycosaminoglycan and its extraction method

A technology of fibrocartilage and glycosaminoglycan, applied in the field of glycosaminoglycan extraction, can solve the problems of adding chemical reagents and high cost, and achieve the effect of low cost, high cost and simple and easy-to-operate extraction method

Active Publication Date: 2021-08-31
SICHUAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Aiming at the above-mentioned problems existing in the prior art, the present invention provides a glycosaminoglycan of fibrocartilage and an extraction method thereof. The glycosaminoglycan is extracted from the fibrocartilage through a simple and easy method, which is low in cost and does not use chemical reagents, effectively It solves the problems of needing to add chemical reagents and high cost in the prior art

Method used

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  • A kind of fibrocartilage glycosaminoglycan and its extraction method

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Embodiment 1

[0020] A kind of fibrocartilage glycosaminoglycan, its extraction method comprises the following steps:

[0021] (1) Take a fresh oxtail vertebra fibrous ring to remove the surrounding fat and muscle tissue, wash it, and perform 5 freeze-thaw cycles in the temperature range of -20-37°C (each freeze-thaw cycle is 24h, respectively, -20°C lasts 12h, 37°C ℃ for 12h), then cut the fibrocartilage into 3-5mm tissue pieces;

[0022] (2) Soak the tissue pieces obtained in step (1) in ultrapure water, stir at 3°C ​​for 36 hours, centrifuge to collect the supernatant, and then freeze-dry (-70°C) to obtain fibrocartilage glycosaminoglycan.

Embodiment 2

[0024] A kind of fibrocartilage glycosaminoglycan, its extraction method comprises the following steps:

[0025] (1) Take the fresh oxtail nucleus pulposus to remove the surrounding fat and muscle tissue, wash it, and carry out 5 freeze-thaw cycles in the temperature range of -20-37°C (each freeze-thaw cycle is 24h, respectively, -20°C lasts 12h, 37°C ℃ for 12h), then cut the fibrocartilage into 3-5mm tissue pieces;

[0026] (2) Soak the tissue pieces obtained in step (1) in ultrapure water, stir at 4°C for 48 hours, centrifuge to collect the supernatant, and then freeze-dry (-70°C) to obtain fibrocartilage glycosaminoglycan.

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Abstract

The invention provides a fibrocartilage glycosaminoglycan and an extraction method thereof, comprising the following steps: taking fresh animal fibrocartilage to remove surrounding fat and muscle tissue, cleaning, and performing 4 to 6 freeze-thaw cycles at a temperature range of -20 to 37°C , and then cut the fibrocartilage into 3-5 mm tissue pieces; soak the tissue pieces in ultrapure water, stir at 3-5°C for 36-60 hours, centrifuge and collect the supernatant, and freeze-dry to obtain fibrocartilage Glycosaminoglycans. The present invention also includes the fibrocartilage glycosaminoglycan prepared by the above extraction method. The invention extracts glycosaminoglycan from fibrocartilage through a simple and easy method, has low cost, and does not use chemical reagents, effectively solving the problems of needing to add chemical reagents and high cost in the prior art.

Description

technical field [0001] The invention belongs to the technical field of glycosaminoglycan extraction, and in particular relates to a glycosaminoglycan of fibrocartilage and an extraction method thereof. Background technique [0002] Acidic mucopolysaccharide, also known as glycosaminoglycan (GAG, glycosaminoglycan), is a long-chain complex sugar molecule composed of disaccharide units composed of hexuronic acid, hexosamine sugar or neutral sugar connected to each other, which can be connected with proteins to form Proteoglycan (proteoglycan). Glycosaminoglycans play an important role in maintaining the elasticity of human skin and connective tissue, and maintaining the stability of the extracellular matrix of cartilage. In previous scientific studies, chemical reagents such as guanidine hydrochloride were used to extract glycosaminoglycans from fibrocartilage, and dialysis was used to remove residual chemical reagents. However, this method has a cumbersome process and compl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08B37/08
CPCC08B37/0003C08B37/0069
Inventor 姜楠应彬彬祝颂松毕瑞野王旻陈浩哲
Owner SICHUAN UNIV
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