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Respiratory tract transmission pathogen enrichment spinning film, sampling mask patch and sampling mask

A technology of spinning membranes and pathogens, which is applied in the field of biomedical materials, can solve problems such as high work risk of throat swab collectors, spread of respiratory infectious diseases, and susceptibility to human factors, so as to improve effectiveness and patient screening efficiency , wide applicability, avoid the effect of throat swab process

Active Publication Date: 2020-11-17
SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Sampling with throat swabs to extract total nucleic acid is a key step in nucleic acid detection. However, when collecting throat swabs from patients, it is necessary to open the mouth to expose the throat area, and the exhaled gas is likely to be virus-containing aerosols and droplets. , so the throat swab collectors work at a very high risk
At the same time, a sampling takes an average of 3-10 minutes, which is easily affected by human factors, resulting in missed diagnosis of positive cases, and even serious consequences for the spread of respiratory infectious diseases

Method used

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  • Respiratory tract transmission pathogen enrichment spinning film, sampling mask patch and sampling mask
  • Respiratory tract transmission pathogen enrichment spinning film, sampling mask patch and sampling mask
  • Respiratory tract transmission pathogen enrichment spinning film, sampling mask patch and sampling mask

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preparation example Construction

[0033] The preparation of the respiratory tract-transmitted pathogen enrichment spinning membrane includes the following steps: preparing protein spinning membrane mouse monoclonal antibody and pathogen-specific mouse monoclonal antibody, and bridging the two through anti-mouse secondary antibody to obtain a membrane with the ability to capture the respiratory tract The spun membrane with the function of transmitting pathogens: that is, the spun membrane that transmits pathogens in the respiratory tract and enriches them.

[0034] This embodiment also provides the respiratory tract transmission pathogen enrichment sampling mask patch and sampling mask prepared according to the respiratory tract transmission pathogen enrichment spinning membrane. In the solution of the present invention, by simultaneously capturing and enriching the pathogens in the exhaled breath of the wearer of the mask, the complicated throat swab process can be avoided, both doctors and patients can be prot...

Embodiment 1

[0037] The preparation of protein spinning membrane comprises the following steps:

[0038] 1. Protein spinning

[0039] Plant protein silk, animal protein silk and insect silk are frozen and ground into ultra-short silk or powder, adding a small amount of polyethylene terephthalate (PET), fully mixed, spinning and doffing and drafting to form a protein spun mesh with a pore size of 50 μm±10 μm.

[0040] 2. Machining

[0041] Cut the protein spinning net into squares of the same size, and lay them vertically to form a non-woven fabric substrate; spray water mist evenly, place the non-woven fabric substrate in the middle of a hot-pressing plate, and perform vertical heat-pressing to form a thickness of 0.5mm ±0.05mm, protein spinning membrane with a porosity of 2% to 5%.

[0042] 3. Assay of protein spinning membrane

[0043]The pore size, thickness and porosity of the protein spinning membrane were tested, and the specific monoclonal antibody was used for indirect ELISA te...

Embodiment 2

[0045] Preparation of mouse monoclonal antibody and anti-mouse secondary antibody:

[0046] 1. The preparation of mouse monoclonal antibody comprises the following steps:

[0047] 1. Mice Immunization

[0048] Protein spinning membrane antigens and pathogen antigens were prepared respectively, and after emulsification, BLAB / C mice were immunized at multiple points through the abdominal cavity and back subcutaneously, for a total of 4 times with an interval of 10 days. Immunization was boosted through the tail vein of rats 2 days before fusion.

[0049] 2. Cell Fusion

[0050] Take the spleen of the immunized mouse, prepare the spleen cell suspension, and mix the mouse spleen cells with the cultured NS1 myeloma cells. Using 50% PEG 4000 as a fusion agent, the mouse splenocytes and NS1 myeloma cells were fused. Fused cells were suspended in selective medium containing 20% ​​newborn bovine serum and cultured for several days.

[0051] 3. Clonal Screening

[0052] The protei...

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Abstract

The invention discloses a respiratory tract transmission pathogen enrichment spinning membrane, a sampling mask patch and a sampling mask, the respiratory tract transmission pathogen enrichment spinning membrane comprises a protein spinning membrane coupled with a capture antibody, and the capture antibody is a specific antibody capable of being combined with various respiratory tract transmissionpathogens in a targeted manner. The enrichment spinning film is composed of a protein spinning film, has the characteristics of multiple layers, multiple holes and air permeability, and is coupled with an antibody for capturing respiratory tract transmission pathogens, external air and pathogens exhaled from the interior of a human body can be efficiently blocked through specific binding of the pathogen antibody, and two-way protection is achieved; the sampling mask provided by the invention can capture pathogens in exhaled air of a mask wearer while blocking the pathogens, so that the pathogens can be enriched in the patch to the greatest extent, sample collection in a long journey process is facilitated, a complex throat swab process is avoided, doctors and patients are protected, the detection pressure is relieved, and the detection efficiency is improved. And the effectiveness of subsequent detection and the patient troubleshooting efficiency are improved.

Description

technical field [0001] The invention relates to the field of biomedical materials, in particular to a respiratory transmission pathogen enrichment spinning film, a sampling mask patch and a sampling mask. Background technique [0002] Respiratory tract infectious diseases refer to diseases caused by viruses, bacteria, mycoplasma, chlamydia and other pathogens invading from human nasal cavity, throat, trachea and bronchi and other respiratory infections. Common diseases include influenza, measles, chickenpox, rubella, meningitis, Mumps, tuberculosis, etc. The disease is often highly infectious, and has the characteristics of multiple transmission routes, fast transmission speed, wide transmission range and general susceptibility of the crowd, and has become one of the most harmful infectious diseases. [0003] Throughout the history of the development of infectious diseases, the epidemic of respiratory infectious diseases has never stopped. Because of its strong infectiv...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A41D13/11A41D27/00B32B9/00B32B9/02B32B9/04
CPCA41D13/11A41D27/00B32B5/02B32B5/26B32B2262/08B32B2307/7145B32B2307/724B32B2535/00B32B2571/00
Inventor 段生宝李勇王红梅丁少华陈晔洲魏双施谢劲松王玉珏刘杰
Owner SUZHOU INST OF BIOMEDICAL ENG & TECH CHINESE ACADEMY OF SCI
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